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作 者 ：朱红芳;;侯瑞贤;李晓锋;朱玉英;侯喜林

期 刊 ：植物科学学报 2011年 29卷 2期 页码：183-187

关键词：不结球白菜；雄性不育；花药发育；石蜡切片；

Keywords:Non-heading Chinese cabbage, Male sterile lines, Anther development, Paraffin,

摘 要 ：以不结球白菜(Brassica campestris ssp.chinensis Makino)雄性不育系及其保持系为试验材料,选择不同发育阶段的花蕾,取其花药,制成石蜡切片和超薄切片,经染色后在电子显微镜下观察。结果表明,不结球白菜雄性不育系与保持系的花药发育有明显的不同:不育系花药发育受阻于花粉母细胞分化期,形成13个药室,并形成正常的四分体小孢子,此时细胞组织逐步解体,形成空腔花药;最后向内皱缩;保持系花粉母细胞能形成正常的四分体,进而形成小孢子,最终形成充满正常花粉粒的花药。

Abstract:Buds selected at different developmental stages of a new germplasm sterile male of non-heading Chinese cabbage and its maintainer lines were studied.Anthers were collected,made into paraffin sections,stained,and observed by electron microscopy.Results show that non-heading Chinese cabbage sterile male lines and maintainer lines exhibited quite different anther development.Male sterile lines in relation to anther development were inhibited at the stage of archesporial cell,produced only 1-3 anther sacs,and formed normal sporogenous cells.The cells gradually disintegrated,formated cavity,and finally shrunk inward.The maintainer archesporial cells formed normal anther sacs,formed sporogenous cell,and ultimately formed anthers with normal pollen.

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