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作 者 ：梁琼, 张燕君, 徐艳琴, 黄宏文, 王瑛

期 刊 ：植物科学学报 2013年 31卷 4期 页码：422-427

关键词：箭叶淫羊藿；地理居群；形态变异；遗传多样性；

Keywords:Epimedium sagittatum, Geographic population, Morphological variation, Genetic diversity,

摘 要 ：箭叶淫羊藿(Epimedium sagittatum)是淫羊藿属中分布最广、形态变异最大、分类学最难处理的一个物种,不同箭叶淫羊藿居群其形态、活性成分等差异较大,质量极其不稳定。本实验选择湖北罗田等12个不同的箭叶淫羊藿地理居群,在武汉植物园进行同园栽培,分析其主要形态数量性状及遗传多样性。结果发现箭叶淫羊藿不同居群在形态上表现出各自明显的差异。基于AFLP数据进行的遗传多样性分析显示,各居群聚类关系与地理分布密切相关,柳州居群(LZ)、西南居群(CL、HH、ZY)、华东华中居群(YT、WN、NF、HS、QZ、SG、LY、LT)依次分出。而形态分层聚类分析显示各居群形态变异复杂,只有部分与遗传多样性有一定的相关性。本研究结果对箭叶淫羊藿分类研究及资源筛选具有重要指导作用。

Abstract:Epimedium sagittatum has wide distribution and the large morphological variation,which makes it difficult to classify.In addition,great differences related to morphological traits and bioactive components exist between different populations with unstable quality.In this study, quantitative morphological characters and genetic diversity of 12 geographic populations of E.sagittatum were determined by a common garden experiment in the Wuhan Botanical Garden,CAS.The results showed there were significant differences in morphological traits.Based on AFLP data,the genetic diversity analysis demonstrated that population relationships were closely related with geographical distribution:Liuzhou population(LZ),Northwest populations(CL,HH,ZY)and Eastern and Central China populations(YT,WN,NF,HS,QZ,SG,LY,LT)were successively separated.But complicated morphological variations were shown by hierarchical cluster analysis of the main quantitative morphological characters and only part of the populations showed a certain relationship with genetic diversity.This study provides important information on the taxonomy and exploration of quality resources of E.sagittatum.

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