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作 者 ：褚晓玲;杨波;高 丽;李洪林;胡磊;杨娜;

期 刊 ：武汉植物学研究 2010年 28卷 2期 页码：199-205

关键词：蕙兰；细菌多样性；16S rDNA；

Keywords:Cymbidium faberi Rolfe, Bacteria diversity, 16S rDNA,

摘 要 ：

以MS基本培养基添加蕙兰菌根浸出液制成的培养基进行分离培养的方法,从野生蕙兰(Cymbidium faberi)根部首次分离到内生细菌.经过分离纯化培养获得纯菌株27株.经过16S rDNA 基因序列测序,并与GenBank数据比对,其相似性均在98%以上,分析鉴定结果表明,存活的22株菌可分为8属14种.分别隶属于伯克氏菌属(Burkholderia)、假单胞菌属(Pseudomonas)、芽胞杆菌属(Bacillus)、Leifsonia属、贪食菌属(Variovorax)、欧文氏菌属(Erwinia)、Duganella属和不动杆菌属(Acinetobacter).对这些菌株进行分离培养及鉴定有助于理解兰花与微生物之间的相互作用关系,为开发利用这些微生物开辟新的思路.

Abstract:Certain entophytic bacteria strains were investigated following isolation from the roots of Cymbidium faberi Rolfe in Hubei province,China.An MS medium+ Cymbidium faberi root extract was used in this study.Twentyseven strains were selected based on the morphology of their colonies.According to 16S rRNA gene sequences analysis and comparison with data from the GenBank,they belonged to 14 species of 8 genera,namely Burkholderia phytofirmans,Pseudomonas jessenii,P.cedrina,P.mohnii,P.fragi,P.koreensi,Bacillus megaterium,B.atrophaeus,B.subtilis,Leifsonia shinshuensis,Variovora paradoxus,Erwinia rhapontici,Duganella zoogloeoides, and Acinetobacter lwoffi. Understanding the relationship between these bacteria and Cymbidium faberi is valuable,particularly in relation to the benefits of these bacteria in the cultivation of Cymbidium faberi Rolfe.

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