---

作 者 ：唐晰;谢东丽;刘潇;蒋际谋;梁文裕;陈伟;

期 刊 ：植物科学学报 2011年 29卷 3期 页码：347-351

关键词：龙眼；种子败育；cDNA-AFLP；TDF；

Keywords:Longan(Dimocarpus longan Lour.), Seed abortion, cDNA-AFLP, TDF,

摘 要 ：采用cDNA-AFLP技术对龙眼(Dimocarpus longan Lour.)正常和败育的种子进行了差异表达分析,并对差异明显的片段进行回收、克隆、测序,以及利用NCBI中的BLAST数据库已知序列进行同源性比对。结果表明:在11条重复性较好的差异片段中有3个Transcript-derived fragments(TDFs)分别与Ca²⁺-ATP酶、纤维素酶、质体ATP/ADP转运蛋白具有较高的相似性,其中TDF2和TDF7下调表达,TDF5上调表达,这可能影响了龙眼种子发育过程中的许多重要代谢途径,从而引起龙眼种子败育;有4个TDFs与一些hypothetical蛋白的序列相似;另有4个TDFs在蛋白数据库中未找到匹配的序列,核酸数据库中比对的结果也显示较高的E-值,说明为非同源基因。

Abstract:cDNA-AFLP technology was applied to identify differentially expressed genes between normal and abortive longan(Dimocarpus longan Lour.) seeds.Transcript-derived fragments(TDFs) identified as differentially expressed genes were eluted,cloned,sequenced and compared with the database using BLAST at NCBI.The results showed that among 11 sequenced fragments,three TDFs presented similarity with Ca²⁺-ATPase,cellulase,and plastidic ATP/ADP transporter,respectively,whose up-regulation or down-regulation expression might influence several important metabolic pathways in developing seeds and led to seed abortion.Four TDFs were similar with hypothetical protein,while the other four TDFs did not match with known proteins in the protein database,while sequence alignment from the DNA database also showed higher E-values,indicating they were non-homologous genes.

---