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作 者 ：高海波;曾幼玲;张富春

期 刊 ：武汉植物学研究 2008年 26卷 6期 页码：634-638

关键词：费尔干猪毛菜；愈伤组织诱导；分化；植株再生；

Keywords:Salsola ferganica Drob., Callus induction, Differentiation, Plantlet regeneration,

摘 要 ：探讨了植物生长调节剂、外植体等因子对费尔干猪毛菜(Salsola ferganica Drob.)愈伤组织诱导、分化与植株再生的影响。结果表明:2,4-D与6-BA组合使用时,在一定浓度范围内均有愈伤组织产生,最佳的诱导培养基为MS+2,4-D 2.0 mg/L+6-BA0.2 mg/L,下胚轴为诱导愈伤组织的最佳材料;愈伤组织继代培养较好的培养基为MS+NAA0.1 mg/L+6-BA1.0 mg/L;愈伤组织不定芽分化培养基为MS+6-BA 0.5 mg/L+NAA 0.05 mg/L;根分化的培养基为1/2 MS+IBA0.2 mg/L,且生根率可达72%。以带柄子叶为外植体,诱导丛生芽的最佳分化培养基为MS+6-BA1.0 mg/L,再生频率可达90.74%。

Abstract:The effects of explants and plant growth regulators on callus induction,differentiation and plantlet regeneration of Salsola ferganica Drob.were studied.The results showed that the best medium of callus induction was MS medium with 2,4-D 2.0 mg/L+6-BA 0.2 mg/L.Hypocotyl was the best explant.The best medium for the subculture of callus was MS+NAA 0.1 mg/L+6-BA 1.0 mg/L.The MS me-dium with 6-BA 0.5 mg/L+NAA 0.05 mg/L was preferable for inducing adventitious shoot.The 1/2 MS medium with IBA 0.2 mg/L was suitable for the root differentiation and the rate of rooting was 72%.The best medium for inducing adventitious shoot of cotyledon with petiole was MS+6-BA 1.0 mg/L and the shoot regeneration frequency was 90.74%.

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