Abstract:In this paper, the Ag-NORs and Giemsa C banding patterns of 5 cultivars in Paeonia suffruticosa and P.suffrugioosa var. papaveracea were reported for the first time. All of their karyotypes are 2n=2x=10=6m+2sm+2st. They have 6Ag-NORs which are different in location on chromosomes. The C banding patterns are also varied in 1, 2, 3 pair chromosomes.It was found that the numbers and locations of terminal bands (T) and AgNORs of chromosomes are same Therefore, T bands are actually N bands, and the satellites shown by normal stained are actually terminal NORs.