---

作 者 ：李学宝;杨学荣

期 刊 ：武汉植物学研究 1989年 7卷 4期 页码：345-350

关键词：水稻；化学诱变剂；生长发育初期；

Keywords:Rice, Chemical mutagen, Seed germinating and growing at first days,

摘 要 ：本文以水稻为材料,研究化学诱变剂dES、NaN₃对种子萌发及幼苗生长发育的影响。用不同浓度的dES、NaN₃处理预浸泡的水稻种子,测定种子的发芽率、发芽势、幼苗高度和根系活力,同时测定其呼吸强度和细胞色素氧化酶活性。结果表明,dES、NaN₃对水稻初期生长发育具有抑制作用。种子经化学诱变剂dES或NaN₃处理后,细胞呼吸强度和细胞色素氧化酶活性明显下降,种子发芽率、发芽势、幼苗生长高度和根系活力也不同程度地降低。诱变剂的剂量愈高,抑制愈强烈。由试验结果可知,化学诱变剂首先影响细胞的能量代谢活动,从而导致种子发芽率降低,幼苗生长迟缓,根系活力下降。

Abstract:Rice seeds were treated with a range of concentration of diethyl sulphate (dES) or sodium azide (NaN₃) after having been presoaked for 16—18 hours with water. The respiratory intensity was determined on second day (seed germinating for 48 hours) and activity of cytochrome oxidase was determined on fourth day (seedling growing for 4 days). The respiratory intensity of germinating seeds and activity of cytochrome oxidase of seedlings decline with the increase of the concentration of dES or NaN₃, and germination rate and germination power of seed, seedling height, root vigor decrease consequentially. The experimental results show that the decreases of germination rate of seed, seedling height, and root vigor are closely linked to the respiratory intensity and activity of cytochrome oxidase in cells in early development of rice. Both dES and NaN₃ at first inhibit the respiration in cells, therefore the changes in seed viability, seedling height, root vigor are caused.

---