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作 者 ：张泽民;朱海涛;王江;陈兆贵;刘芳;宛新杉;张景六;张桂权

期 刊 ：作物学报 2006年 32卷 11期 页码：1737-1741

关键词：水稻；多分蘖突变体；T-DNA 插入；遗传分析；

Keywords:Rice, Multi-tiller mutant, T-DNA insertion, Genetic analysis,

摘 要 ：在筛选和鉴定水稻T-DNA(含Basta抗性基因Bar)插入纯合体的过程中，观察到一个多分蘖的突变体。其分离群体中多分蘖和正常分蘖两种植株类型的分离比率为3∶1，符合1对基因的显性遗传。Basta 抗性检测、PCR分子检测及Southern杂交证实，该突变体是由单一T-DNA插入引起的，多分蘖性状与T-DNA共分离。该突变材料可用于插入座位的

Abstract:T-DNA tagging has been used to isolate genes in higher plant. In this study, a multi-tiller mutant caused by T-DNA insertion in rice was identified. Genetic analysis of the mutant showed that the two types of phenotype, multi-tiller and normal tiller in the segregating populations derived from the T

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