本研究首次报道了发根农杆菌介导的向日葵遗传转化和毛状根形成的优化条件。由发根农杆菌诱导向日葵子叶和下胚轴形成的根在含卡那霉素（200 mg/L，kanamycin）的选择培养基(1/2 MS)上选择，12 d后存活的根转到无激素培养基上能快速生长，经分子检测证实其为毛状根。同时着重研究了不同诱导条件对向日葵毛状根形成的影响。结果发现适用于毛状根形成的最优条件为菌株R1205，菌液浓度（OD600）1.0，15 min感染，共培养3 d，培养基pH 6.5，30 μmol/L乙酰丁香酮（acetosyingone）。在此条件下，子叶和下胚轴的毛状根形成率分别达到23.7％和81.6％。
The transformation of Helianthus annuus L. mediated by Agrobacterium rhizogenes was reported. Almost all roots induced by different Agrobacterium rhizogenes bacterial strains which could survive in the selection medium (1/2 MS) containing 200 mg/L kanamycin were putative hairy roots. The effects of bacterial strains, bacterial concentrations, acetosyingone, silver nitrate and co-cultivation pH on sunflower transformation were investigated. For sunflower transformation, the optimal conditions were strain R1205, 1.0 bacterial concentration (OD600), 15 minutes- infection, co-cultivation for 3 days as well as 30 μmol/L acetosyingone (without silver nitrate) and pH 6.5. Under this condition, the hairy root production frequency was 23.7% (cotyledon) or 81.6% (hypocotyl).
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