全 文 ： ACTA AGRONOMICA SINICA 2008, 34(1): 84−88 http://www.chinacrops.org/zwxb/
ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@chinajournal.net.cn

:

  (2006 kz 05-G3)
 :

(1983–),    !#$%&()*+,-./0E-mail:liusulan1202@163.com
* 1234(Corresponding author):

567(1963–), 8 9 :; 9<= !#$%&()0E-mail: jiwan-
quan2003@126.com
Received(>?@A): 2007-07-19; Accepted(BC@A): 2007-08-08.
DOI: 10.3724/SP.J.1006.2008.00084
 N9628-2  SSR
   *
(  ,  712100)
 :     Am9,   160 , !
 160 # $%, &()*+, - N9628-2 ./01*2345 1* 4 6789:;
<=> N9628-2 ?@ABCDEFGHIBCDJKLMNO, .P-CDQ5LRS SSRNOLR; 160TU 225 N9628-2 , F1).V78, F2)L
WXYVZ[ 3\1, 7= N9628-2B] 1.^BCD_`;a 208. SSRbc. 160 × N9628-2 F2
)LWd 142efghi, j8kl 6Am SSRkn Xwmc553S Xwmc684opSTqrst
uB, !BCDJK, EFvWLwx 10.99S 7.43 cM, 7=CDyzkl 6A{|m; *}~L
€ 6‚dƒ„…S†…Q5‡ˆ, Q$‰ŠBCD‹ko 6AS;JK SSRNOSŒ1pLR
7=, PCDyz‚l   Y39, ŽlsCD, yzx$e‘CD;
:  ;  ; ; BCD; SSRLR
SSR Analysis of Powdery Mildew Resistance Gene in a New Germ-
plasm N9628-2 of Triticum aestivum L.
LIU Su-Lan, WANG Chang-You, WANG Qiu-Ying, and JI Wan-Quan*
(College of Agronomy, Northwest Sci-Tech University of A & F, Yangling 712100, Shaanxi, China)
Abstract: Powdery mildew, caused by Blumeria graminis D C f. sp. tritici (former Erysiphe graminis f. sp. tritici), is one of the
devastating diseases of wheat (Triticum aestivum L.). So far, a total of 34 resistance genes have been formally named. However, very
few of them are used in wheat production due to resistance lose and close linkage to ill agronomic traits. The new resistant germplasm
N9628-2, which was derived from the backcross of Am9 (F1 progeny of the cross between tetraploid wheat-Aegilops amphidiploid
and a sensitive wheat cultivar “Shaan 160”) and Shaan 160, showed highly resistant to Guanzhong 4, the prevailing Blumeria
graminis f. sp. tritici race in Shaanxi province. In the present study, we aimed to identify the resistance gene in N9628-2, and locate it
on wheat chromosome. The F1 (308 plants) and F2 (275 plants) populations derived from crosses of N9628-2 and highly susceptible
cultivars Shaan 160 and Shaanyou 225 were inoculated with powdery mildew race Guanzhong 4 at the seedling stage for resistance
identification. The parents and F2 individuals were used for gene location with 208 pairs of SSR markers including 38 pairs polymor-
phic marker between two parents, and the result was verified by analyzing Chinese Spring nullisomic-tetrasomic and ditelosomic
lines. According to inoculation test, the resistance to powdery mildew in N9628-2 was controlled by a single dominant gene (χ2 =
0.038 and 0.068 in Shaan 160 × N9628-2 and Shaanyou 225 × N9628-2, respectively; χ20.05 = 3.84). Two markers Xwmc553 and
Xwmc684 on chromosome 6A generated polymorphic DNA fragments between the resistant and susceptible pools, indicating the
resistance gene might be located on chromosome 6A and linked to the two markers. The resistance gene was further located on chro-
mosome 6AS by the absence of the above polymorphic DNA fragments only in Chinese Spring 6A nullisomic-tetrasomic and 6AL
ditelosomic lines. The genetic distances between the resistance gene and the two markers, calculated by Kosambi’s formula, were
10.99 (Xwmc553) and 7.43 cM (Xwmc684) respectively. Our research found that the resistance gene in N9628-2 was probably a new
gene differing from the reported resistance genes PmY39, PmPS5B (Pm33), and PmPS5A.
 1 :   N9628-2  SSR 85


Keywords: Triticum aestivum L.; Aegilops umbelluata; Powdery mildew; Resistance gene; SSR analysis
 Blumeria graminis D C f. sp.
tritici ( Erysiphe graminis D C f. sp. tritici Marchal)
 ,  1200 hm2[1],
 !#$% & 34 
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=J (R>, STDUVWX
,YZ[\]^ ()*, _`(
)*aQ=J(R> bcd`eA_`
)* fghijk., l[Amnopq3Y
Zrstu_`)*vYZ (Triticum
aestivum L.)wx yDUfgz{[5]|}~€
-‚~‚ƒA„…†‡ˆ‰Š‹n
opq Am93ŒR>Ž 160rs, F13
Ž 160 s, ‘’A“>”3”•–m
—˜^Ž|™[Vš›œ>™[ 4 ž
u„… J>Ÿ N9628-2[6]
 ¡¢£¤¥l SSR¢£¤uaQ
=(R>¦u(J)*4§2\ bc¨©
h¤z{ , !ª«¬V@A,(­
®¯­®eA SSR ¢£¤, –m( ° ±
²(BSA)^ N9628-2 ³œ()*´µq¶·,
¸¹º[»¼\q½qhn¾q¿À^l³œÁ
¶· , aÃÄheAÅ(` , PÆÅ()*³
{Ç ÈÉ­®h@AÊË)O
1 
1.1 
1.1.1       eA(¿À N9628-2
hŒR>Ž 160¦ŽÌ 225  ÍÎXrsÏmŽ
160×N9628-2hŽÌ 225×N9628-2, ÐÑ F1 308Òh
F2 275Ò Í^rsÓ¯Æl F1h F2³œ(
Ô¶h()* SSR  ¡¢£, Pˆ‰Š
‹ Y39¦†‡ PS5¦†‡ ˆ‰Š‹no
pq Am9a(^Õ’A[»¼ÆlÖ 6× Ø
`Ù? 3\q½qÚ(CSN6AT6B¦CSN6BT6A
hCSN6DT6A)h 6n¾qÚ(CSDT6BL¦CSDT6BS¦
CSDT6AL¦CSDT6AS¦CSDT6DL h CSDT6DS ³
œ()*´µq¶·P?ÛÁ¿ÀÜ|}~
€-‚~‚ƒÝÁÞÊË
1.1.2   ’A™[Všߛœ
>™[ 4, |}~€-‚ÛÁàႃÊË
1.2 
’AÞâãäåæç>gèéê¿Àë,ì
íî[ , ïãðñòó{ôõ , åæö÷ç>
ßø¡, <åæùúÞâQí 15 d ûü, ý 
, þÕ 0~4
 
¢³œ([7], 0~2

a(, 3~4
a, 3 d‘{
1.3 SSR 
1.3.1  DNA  ’A CTAB g[8]Ê
Ó¯h F2 °ÙqÒ )*Ï DNA
1.3.2 SSR  A °Ùq Ï ±g
(bulked segregation analysis, BSA)[9] Ô¶3()
*9: ¢£þ Roder[10]¦Guyomarc[11]h
Somers[12]  m 208 ^ SSR Á r> F2
ïãç>Ô¶(,  F2( °Ùqè 10
Ҍ(Ò DNAm(², 10Ҍ
Ò DNAm², P(Ó¯¦Ó
¯¦(²¦² DNAa“ SSRÁ
1.3.3 PCR  蓘 D.
 SSR  ¡¢£< F2( °Ùq Ò DNA
[³œ PCR@< Perkin2Elmer 480
 !?³œ, @mϦ#$Æ%
&fgÜ( Wang[13] fg
1.3.4    )*+»~,×
GrainGenes-./0 (http://wheat.pw.usda.gov/cmap/),
è“Ñ1 SSR  ¡¢£¶·<´µq?, P[
»¼ÆlÖ 6× Ø`Ù? \q½qÚhn¾q
Ú SSR ¡¢£23–4³œê5
1.4 
χ
26(│A−3a│−2)2/3n, Aa7t893:, aa
;t893:, n=A+a[14]
)*y-<g=>()*3?@Áz
A BÏC[15], þ Kosambi D$[16]èBÏCwEa
MNF°(cM)
A MapDraw V2.1GH[17]IX)*9:J
2 
2.1 N9628-2
ç>Ô¶–47K, N9628-2^™[ 4>ž
u„…, Ž 160¦ŽÌ 225žuŒ, òrs F1
ÜžuŒ( , F2 Ùq (¦ÛÒLM Ía
86      34

10834 ((ÒN 1Ò 2
_Oa 0h 0;, Ò
N 2Ò 3
_Oa 4
)h 10132 ((ÒN 2Ò 1

, 1Ò 2
_Oa 0h 0;, ÒN 3Ò 3
_O
a 4
) P χ22ê, χ2: Ía 0.038 (Ž 160 ×
N9628-2)h 0.068 (ŽÌ 225 × N9628-2), Ü, χ20.05
(df =1, χ20.05 = 3.84), Qm 31  °LM, žR
N9628-2^™[ 4 ( 1^7)*WX
2.2 N9628-2 SSR 
< 208 ^ SSR Á[D 38 ^§˜.Á, l[ 6A ? ò^Á Xwmc553 h
Xwmc684<Ó¯h(¦²zA˜3(•™
?ST, UÇVWXò^Á^@ SSR ·Y3
()*D9:™ÚA Xwmc553(J 1)h
Xwmc684(J 2)^Ž 160×N9628-2 F2 142Ò³
œh%&, –4(ž 1)PKosambiD$=>, SSR
·Y Xwmc553h Xwmc6843()* MNF°
 Ía 10.99 h 7.43 cMè()*Z% a
PmY39-2, l3 SSR ¡¢£ MN9:J[J 3
AŽÌ 225×N9628-2 F2 133 (Òê5,
3( °–4)¯•Q(ž 2)
2.3  
%&u, NCSN6AT6BhCSDT6AL_, ¿À]D!^ ?@_T(J`), aR3()*
9: SSR ·Y·, 6AS, bcè N9628-2 (
)*¶·, 6AS
2.4 N9628-2
nopq Am9 Ó¯†‡ PS5 hˆ‰Š
‹ Y39^ß]D(a&d¶wx1Y

 1 SSR Xwmc553 160 × N9628-2 F2 
Fig. 1 PCR profile of Xwmc553 in F2 population of Shaan 160 × N9628-2
M: DL2000; 1:  160; 2: N9628-2; 3: ; 4: ; 5~11:   ;
12~16:   ; 17~20:  
M: DL2000; 1: Shaan 160; 2: N9628-2; 3: resistant bulk; 4: susceptible bulk; 5–11: individuals with the specific band from resistant
parent; 12–16: individuals with the specific band from susceptible parent; 17–20: individuals with both resistant and susceptible bands.

 2 SSR Xwmc684 160×N9628-2 F2 
Fig. 2 PCR profile of Xwmc684 in F2 population of Shaan 160×N9628-2
M: DL2000; 1:  160; 2: N9628-2; 3: ; 4: ; 5~10:   ;
11~15:   ; 16~19:  
M: DL2000; 1: Shaan 160; 2: N9628-2; 3: resistant bulk; 4: susceptible bulks; 5–10: individuals with the specific band from resistant
parent; 11–15: individuals with the specific band from susceptible parent; 16–19: individuals with both resistant and susceptible bands.
 1 SSR Xwmc553 Xwmc684 160×N9628-2 F2
Table 1 SSR markers related to resistance in F2 population from Shaan 160 × N9628-2
 Resistance plant

 Susceptible plant

SSR
SSR marker A A type AB AB type B B type

A A type AB AB type B B type
Xwmc553 43 62 4 7 2 24
Xwmc684 32 71 5 1 4 29
A:   ; B:   ; AB:  
A type: specific band from resistant parent; B type: specific band from susceptible parent; AB: both resistant and susceptible bands.
 1 :   N9628-2  SSR 87


 2 SSR Xwmc553 Xwmc684 225×N9628-2 F2  
Table 2 SSR markers related to resistance in F2 population of Shaanyou 225×N9628-2
 Resisitant plant

 Susceptible plant

SSR
SSRmarker A A type AB AB type B B type

A A type AB AB type B B type
Xwmc553 43 54 3 2 5 26
Xwmc684 40 59 2 3 4 25
A:   ; B:   ; AB:  
A type: specific band from resistant parent; B type: specific band from susceptible parent; AB type: both resistant and susceptible bands.


 3 6A  PmY39-2
Fig. 3 Location of SSR markers Xwmc553 and Xwmc684 on
wheat chromosome 6A
 N9628-2  ,  Y39
PS5Am9 160  N9628-2 ,
  2  SSR  Xwmc553 
Xwmc684 !#($ 5), %& Xwmc553(
N9628-2  )*+, PS5  Y39 -
./0, 123456 7%
&Xwmc684(N9628-2 )*
+,89 PS5 :;/0, 2<,=>?@ Y39 
/0, ABC D Y39, 6EFG
D Y397

4 Xwmc553(A) Xwmc684(B) !#
Fig. 4 Performance of Xwmc553(A) and Xwmc684(B) in Chinese Spring nullisomic-tetrasomic and ditelosomic lines
M: DL2000; 1: Shaan 160; 2: N9628-2; 3: CS; 4: CSN6BT6A; 5: CSN6DT6A; 6: CSN6AT6B;
7: CSDT6BL; 8: CSDT6BS; 9: CSDT6AL; 10: CSDT6AS; 11: CSDT6DL; 12: CSDT6DS.


5 Xwmc684(A) Xwmc553(B) N9628-2$%&
( !#
Fig. 5 Performance of Xwmc684(A) and Xwmc553(B) in
N9628-2 and its parents
 !# $
The arrows show the specific bands in resistant plants. M: DL2000;
1: PS5; 2: Am9; 3: Y39; 4: N9628-2; 5: Shaan 160.
3 
HI, J; 34 KLJ 50 MN
(OPQRK)STUVW, XYZ,
[ 3A2D4D6D\J 17]^_`a[18]7bc0
  Ddefg
hie, ^_`jkl, Fmno
pfghie qrsd
, 12tu 6vwl,
xy,zhc{|7}~€‚|&ƒ
h„…†‡ˆ‰ŠR[19-20]‹Œ(T. arthli-
cum)Ž(T. durum)d(T. aestivum)R
>?@i(Aegilops)Yh‘, z’“ 20 ”•
–—`, XY•–—`”˜™ š›œ;
žŸ6,  Y•–—`6bq¡7
¢£¤R[5,21-22], Y39c0“  PmY39,
¥¦§  3 ¨©ª Xgwm257
Xgwm296Xgwm319, X3«KDY392US
a7¬\ , ­®¯, PS5 c0“ 
PmPS5BPmPS5A[23], °±K,2BL2ALa, b
TUVW Pm33  PmPS5B ¨©ª
Xwmc317Xgwm111Xgwm382Xgwm526, v
88     % 34&

w²³° 1.12.24.0  18.1 cM; FG Pm4
RKPmPS5A¨©ªXgwm356, ²
³ 10.2 cM7´µ™ N9628-2 3 ¶·89
6#,  160Ÿ¸ , Y39 PS5
, 6¹FG D Y39 œ PS579…†
PmY39PmPS5B(Pm33) PmPS5A¨©ª
™¸º !#, »¼«:;(/)*]+, AB
N9628-26b; «½-, FG
¾¿ , 2À Xwmc684 < 
D Y39, AB6EFG D Y39, Áp
N9628-2‡Â+ ÃVW PmY39-27
ÄD‡¦§6vw²³ÅÆ,
¯3ÇDÈÉÊzh, ËÌ ­
ÈÍÎÏÐC6ÑÒœJ³
,xC6Ó±ÔF!7
4 
ÕSSR#p¿hÖN9628-2 
±K, 6AS a, ¥4±C6FG D Y39,
Á½-Db×Ø , FG¾¿,
ÃVW PmY39-27SSR  Xwmc553  Xwmc684
 PmY39-2vw²³°¾ 10.99 7.43 cM7
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