全 文 ： ACTA AGRONOMICA SINICA 2008, 34(1): 106−110 http://www.chinacrops.org/zwxb/
ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@chinajournal.net.cn

:

(1963−), ,  ,  , , 
Received( !): 2006-12-06; Accepted(#$!): 2007-06-14.
DOI: 10.3724/SP.J.1006.2008.00106
 SQ-1  

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Male Sterility Induced by Chemical Hybridizing Agent SQ-1 in Common
Millet
CUI Xiu-Hui
(Nenjiang Institute of Heilongjiang Academy of Agricultural Sciences, Qiqihaer 161041, Heilongjiang, China)
Abstract: Effect of chemical hybridizing agent SQ-1 on common millet was studied with the split plot design. The different
treatment stages and dosages of SQ-1 were designed as main plot and sub-plot respectively. Male sterility induced by SQ-1 was
evaluated according to the three indexes: male sterile rate, crossing rate, and seed-setting rate of natural pollination. The results
showed that treatment stages, dosages of SQ-1 and the interaction between them affected male sterile rate and crossing rate sig-
nificantly or highly significantly. The induced male sterile rate and crossing rate were both over 95% with treatments of different
dosages (4.0 and 5.0 kg ha−1) in the differentiation stage of the anlage of gynoecium and stamen, and also under the dosage of 5.0
kg ha−1 at the primary stage of forming pollen. The seed-setting rate of natural pollination was not affected significantly by the
treatment stages, but affected significantly by the dosages and interaction between the treatment stages and dosages, and decreased
with the dosage. Considering all the three indexes and the cost of production of hybrid seed, the optimum treatment stage is the
differentiation stage of the anlage of gynoecium and stamen and the optimal dosage is 4.0 kg ha−1.
Keywords: Chemical hybridizing agent; Common millet; Male sterility; Heterosis
(chemical hybridizing agent, CHA)
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Table 1 Variance analysis of sterile rate, hybridization rate and natural
pollinate fertility under the different doses of SQ-1 at different stages

Sterile rate


Hybridization rate
 
Seed-setting rate for natural
pollination


Source of variance
df
SS MS F value


SS MS F value




SS MS F value
 Main plot

 Block 2 0.65 0.33 0.62 2.81 1.40 1.73 0.89 0.45 0.71
 Stage (A) 4 1893.86 473.47 893.34** 4229.34 1057.34 1305.36** 40.56 10.14 2.47
 Ea 8 4.20 0.53 6.48 0.81 32.88 4.11
 Sub-plot






 Dosage (D) 4 2900.48 725.12 496.66** 4010.06 1002.52 276.94** 1607.98 402.00 204.06**
 A×D 16 527.96 33.00 22.60** 1356.66 84.79 23.42** 213.93 13.37 6.79**
 Eb 40 58.24 1.46 144.90 3.62 78.86 1.97
  Total variation 74 5385.39 9750.24 1975.10
*:
0.05 ; **: 0.01 !*: significant at 0.05 probability level; **: significant at 0.01 probability level.

108     34#

2  SQ-1  
Table 2 Significant difference of sterile and hybridizing rates with SQ-1 dosages at different treatment stages

Stage

Sterile rate ($)

Hybridization rate ($)

Dosage (kg hm−2)

Sterile rate ($)

Hybridization rate ($)
A5 84.2±0.42 dD 65.9±0.52 eE 1.0 98.7±0.70 aA 95.4±1.10 aA
A4 94.4±0.42 bB 85.6±0.52 cC 2.0 95.7±0.70 bB 87.8±1.10 bB
A3 97.0±0.42 aA 93.1±0.52 aA 3.0 93.5±0.70 cC 83.1±1.10 cB
A2 96.2±0.42 aA 90.8±0.52 bB 4.0 88.4±0.70 dD 75.4±1.10 dC
A1 89.4±0.42 cC 75.1±0.52 dD 5.0 83.0±0.70 eE 69.9±1.10 eC
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7823459; A3: 6782345:; A4: 1 ;45<=9; A5: 1 ;45<=:!
Values within a column followed by a different capital letter are significantly different at 0.01 probability level. Values within a column
followed by a different lowercase letter are significantly different at 0.05 probability level. A1: differentiation stage of spikelet anlage; A2:
primary differentiation stage of the anlage of gynoecium and stamen; A3: late differentiation stage of the anlage of gynoecium and stamen; A4:
primary differentiation stage of pollen formation A5: late differentiation stage of pollen formation.
3  ! 
Table 3 Sterile and hybridizing rates under different dosages of SQ-1 at different stages($)
3.0 kg hm−2

4.0 kg hm−2

5.0 kg hm−2


Stage 
Sterile rate

Hybridization rate

Sterile rate

Hybridization rate

Sterile rate

Hybridization rate
A1 88.1±0.65 cC 70.7±1.01 cC 90.7±0.65 cC 73.5±1.01 dD 93.5±0.65 dC 85.7±1.01 cC
A2 96.1±0.65 bB 89.8±1.01 bB 98.7±0.65 aA 94.9±1.01 bA 100±0.65 aA 100±1.01 aA
A3 99.4±0.65 aA 98.0±1.01 aA 98.6±0.65 aA 97.1±1.01 aA 100±0.65 aA 100±1.01 aA
A4 96.2±0.65 bB 87.0±1.01 bB 96.8±0.65 bB 90.0±1.01 cB 99.2±0.65 bB 95.2±1.01 bB
A5 79.2±0.65 dD 57.3±1.01 dD 90.4±0.65 cC 75.1±1.01 dD 95.6±0.65 cC 79.8±1.01 dD
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Values within a column followed by a different capital letter are significantly different at 0.01 probability level. Values within a column
followed by a different lowercase letter are significantly different at 0.05 probability level. Abbreviations as in Table 2.


1  #
Fig. 1 Sterility and hybridization rate in different stages ×
different doses

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Seed-setting rate for natural pollination arcsin−1 P
 
Seed-setting rate for natural pollination
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