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作 者 ：莫结胜;刘杰;刘公社

期 刊 ：作物学报 2001年 27卷 01期 页码：85-90

关键词：杂交油葵；种子纯度；RAPD；

Keywords:Oilseed sunflower hybrids, Seed purity, RAPD,

摘 要 ：从杂交油葵A15及其亲本中提取基因组DNA，用180个RAPD随机引物进行扩增，从中筛选出3个可将亲本和子代区分开的引物OPD09、OPD12和OPK12。OPD09产生亲本互补的特征带OPD09-1470bp、OPD09-870bp;OPD12产生母本特征带OPD12-1230bp,OPK12产生父本特征带OPK12-1540bp、OPK12-940bp，上述谱带均在子代中出现。以单引物(OPD09)和双

Abstract:The genomic DNAs extracted from young leaves of oilseed sunflower hybrid A15 and its parents were analyzed using polymerase chain reaction (PCR) with arbitrary 10-mer primers to generate random amplified polymorphic DNA (RAPD) markers. Among 180 RAPD primers, three primers, OPD09, OPD12, OPK12, whic

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