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作 者 ：王玲;宋运淳;宁顺斌;李立家;刘立华

期 刊 ：作物学报 1999年 25卷 05期 页码：639-642

关键词：玉米；rip基因；pal l基因；原位杂交；

Keywords:Maize, Gene rib, Gene pal1, In situ hybridization,

摘 要 ：本文以玉米自交系黄早四为供试材料，采用cDNA为探针，对低拷贝小片段的rip和pall基因进行了定位。用生物素标记和相应的酶联级联放大检测系统，在第8和第3染色体长臂上检测到rip基因的杂交信号，信号与着丝粒百分距离分别为23.66±1.32和72.47±3.16。在第5和第4染色体长臂以及第2染色体短臂上检测到pal l基因位点，信号与着

Abstract:In this paper，the low copy genes rip and pall were physically mapped by in situ bybridization. With biotinylated cDNA probes of the two genes and DAB detection，the signals of the rip gene were detected in the long arms of the eighth and third chromosomes. The percent distances from centromere to d

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