用8个微卫星位点，63个等位基因，研究了8个栽培型紫花苜蓿品种（Medicago sativa L.），320个单株群体内和群体间的遗传变异。首次提出用四倍体方法统计紫花苜蓿微卫星位点的等位基因频率，以等位基因频率为基础，得到遗传杂合度、有效等位基因数、标准遗传距离和NJ（Neighbor Jointing）聚类图。结果表明这8个材料在群体内和群体间均具较高变异。通过0/1法和四倍体方法得到的遗传杂合度之间的显著性检验（P<0.01），表明四倍体方法更适于紫花苜蓿微卫星多态性研究。
With 8 SSR (simple sequence repeat) loci and 63 alleles, the genetic variation of within and between 8 alfalfa (Medicago sativa L.) populations, including 320 individuals, was analyzed. The autotetraploid method was first developed and used to analyze the allele frequencies in autotretraploid alfalfa with SSR markers. Based on the allele frequencies, the genetic heterozygosity, effective allele number, standard genetic distance, and NJ(Neighbor Jointing) dendrogram were obtained, including the high genetic diversity of within and between the 8 alfalfa populations. The genetic heterozygosities from the 0/1 method and the autotetraploid method were significantly (P<0.01), showing that the autotetraploid method is more scientific to analyze SSR polymorphism in alfalfa.
全 文 ：