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作 者 ：刘东;朱利泉;王小佳

期 刊 ：作物学报 2004年 30卷 05期 页码：427-431

关键词：甘蓝；自交不亲和；S受体激酶SRK；臂重复蛋白ARC1；

Keywords:Brassica oleracea L., Self-incompatibility, S-locus Receptor Kinase, Arm Repeat Containing 1,

摘 要 ：以甘蓝自交不亲和系20010197的植株为材料，提取幼苗中的gDNA及花蕾期的柱头、子房、花瓣、叶片、根的mRNA，并对mRNA进行反转录合成cDNA，然后以gDNA与cDNA为模板扩增ARC1蛋白编码序列。结果表明只在gDNA与花期柱头mRNA的反转录cDNA中扩增出产物，而在其他组织中无任何产物。这可能说明甘蓝ARC1蛋白编码序列是在柱头特异性表

Abstract:Cloning by PCR and sequencing of ARC1Boler gene from genomic DNA and stigma cDNA in Brassica oleracea L. of self-incompatibility are immensely important. It helps to understand of ARC1 gene mediating in self-incompatibility signaling process. In this paper, ARC1Boler gene was amplified and sequenced

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