利用雄性不育是实现谷子杂种优势利用最经济、有效的途径之一。为了寻找与不育基因Msch紧密连锁的分子标记，提高不育系的选育效率，本研究构建了Msch不育/可育近等基因系（NILs），通过对400对AFLP引物组合进行筛选，找到了与不育基因紧密连锁的两个AFLP标记（P17/M37224和P35/M52208），与不育基因的遗传距离分别是2.1 cM和1.4 cM，而且位于不育基因的同一侧,标记间相距0.7 cM。这两个AFLP标记可有效用于分子标记辅助选择育种。
Foxtail millet (Setaria italica (L.) Beauv.) is an old and important food crop in China, India and other countries of East Asia. It is still grown as birdseed in Europe and as fodder plant in South America. As a drought resistant crop, foxtail millet has been playing an important role on dry farming. Utilizing heterosis is one of the most economic and effective approached to improving yield of crops, and the use of male sterility is an economic and effective way to utilize heterosis in foxtail millet. After seventies of last century, obvious progress has been made on the study of male sterility, and several sterile germplasms of foxtail millet were produced. “Chifeng sterility”, a nuclear genic interaction type of dominant male sterility in foxtail millet, was discovered from the hybrids of Australia millet and Tulufan millet. To identify AFLP markers linked to the gene “Msch” conferring the dominant male sterility, sterile bulk (SB) and fertile bulk (FB) prepared from the NILs were subjected to polymorphism screening in AFLP analysis. A total of 400 random AFLP primer combinations were used to screen polymorphic markers. Two markers (P17/M37224 and P35/M52208, Fig.2 and 3 ) tightly linked to the dominant male-sterility gene were identified, with a map distance to the gene of 2.1 cM and 1.4 cM, respectively. These two markers may play an important role in the identification of Msch lines through marker-assisted selection in foxtail millet to improve the breeding efficiency.
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