---

作 者 ：周邗扬; 佘建明; 陆维忠; 吴鹤鸣

期 刊 ：作物学报 1991年 17卷 05期 页码：340-345

关键词：油菜；下胚轴；原生质体培养；植株再生；

Keywords:Brassica napus, Hypocotyl, Protoplast culture, Plantlet regeneration,

摘 要 ：以甘蓝型油菜下胚轴游离获得原生质体。经液体-固体双层培养,原生质体分裂、增殖。约四周左右形成细胞团和肉眼可见的小愈伤组织。然后转入固体培养基中增殖。待愈伤组织长到直径约5mm 大小转入分化培养基中。两周后形成绿芽,并诱导生根,获得再生植株。

Abstract:Protoplasts were enzymatically isolated from the hypocotyl of Brassica napus and werecultured in liquid-solid DPD medium.In 4 weeks of culture,mass of regenerated cellsand small calli were formed.The calli were placed on MS medium for proliferation.Whengrew up to 5 mm in diameter,they were transfere

---