Abstract:The present paper deals with the karyotype analyses of Chamaecyporis lawsoniana, Ch.obtusa and Ch.pisdera. The karyotype formulas of the species are K(2n)= 22= 22m, 20m+2sm and l4m+8sm respectively. After making comparison among the karyotypes of 6 representative species in Chamaecyporis with related data of geography and fossil, the authors think that the spe- cies lived in North America might be more primitive than that in East Asian. In the Cupressoideae Chamaecyporis may be the most advanced one, Cupressus the most primitive and Fokienia and x Cupressocyparis may be the intermediate taxa. The evolutionary positions of Cupressus funebris and Fokienia hodginsii are also discussed in the paper.