Abstract:This paper reports six alkaloids,colchicine(Ⅰ),2-demethylcochicine(Ⅱ),2-demethyldemecolcine(Ⅲ),2-demethylcoclchifoline(Ⅳ),2-demethyl-β-lumicolchicine(Ⅴ),and β-lumicolchicine(Ⅵ) isolated from the flowers of Colchicum autumnale which was introduced from Europe to Zhaotong ,Yunnan,China.