Abstract:The present paper deals with the karyotype analyses of Thup Plicata, T. occidentalis and Calocedrus decurrens. The karyotype formulas of the species are K(2n)= 22= 16m +6sm(2SAT), 20m(2SAT)+2sm and 20m(2SAT)+2sm respectively. The karyotype of T.plicata is reported for the first time. The comparison among the karyotypes of 10 genera in Thujoideae (Ta- ble 2, Fig.2) shows that the genera in the Southern Hemisphere (including Callitris, Aclinostrobus, Libocedrus, Microbiota and Widdringtonia) and Tetraclinis and Platycladus in the Northern Hemi- sphere may be the most primitive, Thuppsis and Thup the most advanced, and Calocedrus inter- mediate. The paper also discusses the classificatory positions of Calocedrus, Platycladus and the southern genera.