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作 者 ：田向荣 欧阳学智 宋松泉

期 刊 ：云南植物研究 2003年 25卷 05期 页码：1-3

关键词：种子；发育；萌发；劣变；程序性细胞死亡；

Keywords:Tomato；Cotyledon explant；Transformation system,

摘 要 ：禾谷类种子胚乳发育过程中的程序性细胞死亡(PCD)主要发生在种子成熟期的后期，并伴随着生物合成的停止和自然脱水；乙烯和活性氧促进胚乳发育中的PCD，而ABA起负调节作用。种子萌发过程中糊粉层降解的PCD被GA、Ca2+和活性氧促进，被ABA和抗氧化剂抑制。种子人工老化和劣变种子萌发过程中可能存在PCD事件，其研究对延长种子的贮藏寿命和提高播种品质具有重要的意义。

Abstract:Aim:To optimize the factors that affect transformation system of tomato cotyledon explant.Method:By orthogonal design of statistics,factors that affect transformation such as the Agrobacterium concentration,infiltration time and transform style were assembled.Result:Best transformation system could be set up by the contidition of 2 days preculture,30-fold-dilution,10-minute-infiltation of Agrobacterium solution,and 2 days co-cultivation.The frenquency of success transformation reached to 25%,higher than average level at present.Conclusion:Factors that affect transformation system of tomato cotyledon explant were successfully optimized.

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