Abstract:The genetic diversity and population differentiation of an endemic Dipterocarp, Vatica guangxiensis, were examined using allozyme analysis of horizontal sliceable starch gel electrophoresis. It is dintributed in Mengla of Southern Yunnan and Napo of Southwest Guangxi only. Aallozyme data for 23 loci of 14 enzymes indicated low level of genetic diversity with the proportion of polymorphic loci(P) ranging from 13.0% to 21.7%, average mumber of alleles per locus (A) from 1.1 to 1.2, average expected (He) and observed (Ho)heterozgosity per locus from 0.056 to 0.130 and 0.055 to 0.130,respectively.