Abstract:A new cholestane glycoside, (22S)-cholest-5-en-1β,3β,16β,22,25-pentaol 1-0-β-D-glucopyranosyl-16-O-β-D-apiofuranoside, together with two known cholestane glycosides were isolated from the tubers of Polianthes tuberosa. Their structures were elucidated on the basis of spectroscopic data.