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作 者 ：郭丽红 陈善娜 龚明

期 刊 ：云南植物研究 2004年 26卷 01期 页码：1-3

关键词：Ca2+；EGTA；抗逆性；谷胱甘肽还原酶；玉米幼苗；

Keywords:Tricholoma matsutake,

摘 要 ：两个抗逆性不同的玉米品种经过CaCl2浸种处理后，能显著提高其幼苗在低温、高温、干旱和盐胁迫下的存活率；相反，Ca2+螯合剂EGTA浸种处理会降低玉米幼苗在上述逆境胁迫下的存活率，表明外源Ca2+处理能增强玉米幼苗对低温、高温、干旱和盐胁迫的多重抗逆性。此外，Ca2+浸种处理能使玉米幼苗在多种逆境胁迫下保持相对较高的谷胱甘肽还原酶(GR)活性，而EGTA处理正好相反，表明GR可能参与了Ca2+提高的玉米幼苗多重抗逆性的调控。

Abstract:A pair of general primer (ITS4ITS5) and a pair of specific primer (TMFTMR) were used to test in the amplification of the internal transcribed spacer (ITS) region of the chromosomal DNA of fruit bodies of Tricholoma matsutake and its 6 isolates,Tricholoma bakamatsutake and its 3 isolates,fruit bodies of Flammulina velutipes,Pleurotus ostreatus and Agaricus bisporus.The primers of ITS4 and ITS5 amplified about 600?bp fragments for all samples.However,with TMF and TMR primers we can amplify about 500?bp fragments only from the fruit bodies of Tricholoma matsutake and their corresponding isolates.The ITS fragments of Tricholoma matsutake fruit body (TF25) and its corresponding isolate (TM251122) were sequenced and aligned.Their sequences showed a 100% homogeneity.Based on these facts,6 isolates designated as TM06112,TM151101,TM251122,TM281121,TM291121 and TM30122 were confirmed to be pure cultures of Tricholoma matsutake.

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