Abstract:The present paper deals with the karyotype analysis of three species in Cupressaceae in-cluding Calocedrus macrolepis var.formosana, Juniperus deppeana and Chamaecyperis formosensis.Their morphology of somatic chromosomes in root tip cells, chromosome parameters and dia-grams are shown is Plate 1, Table 1 and Figure 1, respectively. The karyotype fomulas of the spe-cies are K(2n)= 22= 22m(2SAT), 22m(2SAT) and 20m(2SAT)= 2sm, respectively. Their chromosome complements are 2n = 22 = 6M2+16Ml, 2L+6M2+14Ml and 4L+6M2+10Ml+2S ac-cording to the standard defined by Kuo et al. (1972) based on relative length. They belong to 1A and 2A type of Stebbins‘ karyotypic asymmetry. The karyotypes of the two former species are re-ported for the first time. Some related cytotaxonomic problems are also discussed in the paper.