Abstract:The present paper deals with the karyotype analysis of Tsuga longibracteata Cheng endemic to China, which is a represent species of subgen. Paleotsuga, for the first time. The somatic chromosomes in root -tip cells of the plant are found to be 2n=24, all with median and submedian constrictions. The 12th chromosome of the karyotype has a long centromere region.