Abstract:Five new iridoidal glycosides, gentioumosides A-E were isolated from the whole herb of Gentiana urnula. Their structures were elucidated mainly by spectral methods. Among which gentioumosides A-C are bis-iridoidal glycosides while gentioumosides D and E are loganin type ones. It is noted that all the glycosides possess a 2,3-dihydroxy benxoyl unit or its derivative in the molecule.