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期 刊 ：植物遗传资源学报 2015年 16卷 4期

关键词：六倍体小黑麦；SSR；遗传多样性分析；

Keywords:Hexaploid Triticale, SSR, genetic diversity,

摘 要 ：摘要：本研究采用已筛选出的多态性较好的分布于六倍体小黑麦21对染色体上的100对SSR引物对从国际玉米小麦改良中心（CIMMYT）引进的339份六倍体小黑麦进行了遗传多样性分析。结果显示，100对引物共检测出323个等位变异，变幅是1～6个，平均等位变异丰富度为3.23；多态信息含量（PIC）的变幅为0～0.748，平均多态信息含量为0.465；平均遗传多样性指数（H’）为0.1439，表明六倍体小黑麦具有较丰富的遗传多样性。同时基于Nei’s遗传距离对339份材料进行聚类分析，可以将供试材料分为6个类群。其中，第Ⅵ类群与其它类群的遗传距离较大、遗传差异较明显。

Abstract:Abstract: 339 hexaploid Triticale from International Maize an d Wheat Improvement Center （CIMMYT）were analyzed using 100 microsatellite markers distributed across the whole Triticale genome. 100 pairs of primers detected 323 alleles, ranged from 1 to 6, with an average abundance of allelic variation was 3.23; polymorphic information content (PIC) ranged from 0 to 0.748, an average of PIC was 0.465, belong to moderate polymorphic loci; average genetic diversity index (H‘) was 0.1439, indicating that the genetic diversity of hexaploid Triticale was relatively rich. Clustering results based on Nei‘s genetic distance showed that the tested materials can be divided into six groups. Among them, genetic distance between groups Ⅵ and others was very obvious.

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