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期 刊 ：植物遗传资源学报 2015年 16卷 5期

关键词：水稻；数量性状座位；种子活力；

Keywords:Rice (oryza stativa L), Quantitative trait locus (QTL), Seed longevity,

摘 要 ：摘要：本文以在2004年构建并保存在种质库10年的186个单株组成的湘743/Katy F2:3群体为材料，在发芽的第5 d和第9 d统计亲本和各株系的发芽率和成苗率，应用由129个标记组成的连锁图谱检测与种子活力相关的数量性状座位（QTL）。一共检测到12个QTLs，共分布在6条染色体的6个区间，单个QTLs对群体性状表型变异的贡献率为5.73% ～47.53%，联合贡献率都是50%。其中，在第8染色体RM152-RM310区间检测到一个主效的QTL，对5 d发芽率和9 d发芽率和成苗率的贡献率分别为12.02%、47.53%、38.64%，来自于湘743的基因增加发芽率和成苗率；第9染色体RM444-RM219区间检测到一个稳定表达的QTL，对5 d发芽率和9 d发芽率和成苗率的贡献率分别为8.85%、7.49%、10.36%，来自于Katy的基因增加发芽率和成苗率；此外，没有检测到显著性的上位性互作位点。

Abstract:Abstract：In this paper, a F2:3 population of 186 linesstored in gene bank for ten years at Hunan Rice Research Institute (HNRRI) which were derived from Xiang 743 / Katy and was tested for germination percentage and normal seedling percentage in the fifth and ninth day at seed germination stage. A linkage map consisting of 129 SSR markers was employed to detect quantitative trait loci (QTL) associated with seed longevity. A total of 12 QTLs were detected, distributing on 6 regions in the genome, with phenotypic variance explanation anging as 5.73% ～47.53% and general contribution as high as 50%. Among them, a major QTL was identified in the interval of RM152-RM310 on chromosome 8, with germination percentage contribution of 12.02% in the fifth day and germination percentage contribution of 47.53% and normal seedling percentage contribution of 38.64% in the ninth day, the allele for Xiang 743 played positive effect. Meanwhile, a stable QTL was detected in the interval of RM444-RM219 on chromosome 9, with germination percentage contribution of 8.85% in the fifth day and germination percentage contribution of 7.49% and normal seedling percentage contribution of 10.36% in the ninth day, the allele from Katy played positive effect. In addition, no epistatic interaction was detected among putative QTLs.

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