摘 要 :通过对马俊榔甜味蛋白Ⅰ和Ⅱ热变性试验,发现MaⅠ在80℃水浴中很快失甜味;圆二色谱证明其主链构象中的a螺旋几乎全部消失了。但 Ma II却能经受长时间保温而不失去甜味;电泳行为亦不发生改变。
Abstract:After a series of heating denatlltation experiments, we found the sweetness of Ma l was irreversibly lost in incubating at 80 0C for 30 minutes, the α-helix of Ma l also disappeared totally and the PAGE beha-vior changed.But the sweetness of Ma Ⅱwasm′t lost at 80oC for 48 h incubation, and its PAGE behavior didn′t change.