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期 刊 ：植物遗传资源学报 2014年 15卷 2期

关键词：水稻；SSIIa基因；糊化温度(GT)；成糊温度(PT)；校准成糊温度(PTm)；直链淀粉含量(AC)；

Keywords:Rice, SSIIa gene, Gelatinization temperature(GT), Pasting temperature(PT), calibrated pasting temperature(PTm), Amylose content(AC),

摘 要 ：RVA常被用来鉴定水稻种质资源和所选优良株系的食味品质，RVA成糊温度（PT）却不能准确反映糊化温度（GT），校准成糊温度PTm能够比较准确地反映GT，但PT的校准范围、PTm衡量稻米GT的准确程度及SSIIa基因型对其的影响等并未见报道。本研究利用遗传背景差异小、双亲糊化温度差异大的B1F8代株系中58个单株为材料，通过检测其SSIIa基因型，将材料分为3类，分别在3种SSIIa基因型的遗传背景下，比较分析PT、PTm、GT，结果表明，SSIIa基因型不同，PT的校准范围明显不同，PT-PTm的均值差异有统计学意义；PT衡量稻米GT的准确率仅为39.66%，而PTm准确率达到89.66%。进一步利用PT和PTm分别表示GT，分析稻米AC与GT关系，得出与一些学者不同的结论：稻米AC与GT呈极显著负相关性。

Abstract:RVA was often used to identify germplasm resources and eating quality of the selected strains, but the RVA pasting temperature(PT) couldn’t accurately reflect gelatinization temperature(GT). Calibrated pasting temperature(PTm) could accurately reflect GT, however, the calibration range of PT, the accuracy rate of using PTm to reflect GT and the influence of SSIIa gene on them weren’t reported in papers. In this study, we use fifty-eight individual plants came from B1F8 breeding line as our materials which had small differences in genetic background, but large differences in gelatinization temperature of their parents. By detecting the genotype(haplotype) of SSIIa gene of the materials, we divided materials into three categories and compared PT、PTm and GT under the genetic background of three different genotype of SSIIa respectively. The main results were as follows: Calibration ranges of PT were apparently different due to the differences existing in genotype of SSIIa, and the average of PT-PTm had very significant difference. The accuracy rate reached 39.66% when we used PT to evaluate GT, while reached 89.66% when we used PTm to evaluate GT. Then using PT and PTm to represent GT respectively, we analyze the relationship between AC and GT of rice, the result showed that AC had a significant negative correlation with GT, which was different with some scholars’ conclusion.

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