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期 刊 ：植物遗传资源学报 2014年 15卷 6期

关键词：芒；表型性状；核心种质；遗传多样性指数；

Keywords:Miscanthus sinensis, phenotypic traits, core collection, diversity index,

摘 要 ：根据555份芒(Miscanthus sinensis)种质资源为研究材料，根据的27个表型性状数据，利用地理来源、植物区系和单一性状进行分组，分别采用分别采用完全随机和系统法进行分组，以简单比例法、平方根法和多样性指数法确定取样数比例，以聚类和随机2种取样方法进行个体选择，构建出了19个具有代表性芒的初选核心种质样本库。通过平均相似系数、性状符合度和数量性状变异系数和遗传多样性指数等4项检测指标对上述19种构建方案进行比较，最终确定了按植物区划分组+多样性指数确定取样数+聚类选择个体为芒初级核心种质构建的最佳方案。通过此法建立起芒的初级种质资源83份，占总资源比例的14.95%，新构建的初级种质资源与总资源性状符合度达到100%。

Abstract:In this study, 555 Miscanthus sinensis germplasm resources were selected as material. Based on 26 phenotypic traits, 19 sampling strategies were used, which consisted of 3 stratification methods such as resource of geographical origin, floristic region and single character , 3 sampling number determination methods such as proportioned, square root and diversity index and 2 individual selection methods such as random and cluster. The criteria against whole collection included mean similarity coefficient, coincidence of traits, mean coefficient of variation of quantitaive traits and mean shannon index. The results indicated that an optimal strategy was set by stratification according to floristic region. determination of the sampling number with genetic diversity-dependent strategy and selecting sample by clusters. By using this strategy, a pre-core collection of Chinese Miscanthus sinensis germplam with a total of 83 accessions was established, carrying 100% phenotypic genetic diversity of the whole collection.

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