Abstract:Abstract Lateral buds of Vanilla planifolia were used as explants and cultured on MS (or VW) medium supplemented with IAA (0.1-0.5 mg/1) and 6-BA (1.0-2.0 mg/1). Subcultures were made every 6-8 weeks. Multiplication ratio is 3. Test-tube plantlets, longer than 10 cm, were obtained by transfering the well grown shoots in the medium without 6-BA. In Jinghong, after planting the plantlets in the field, in 31 months, some of the plantlets bloomed.