Abstract:Wide relatives of maize have rich genetic variations, which are valuable materials for maize germplasm resources and genetic basis. By a large number of hybridization between ordinary maize and tetraploid perennial maize, a partially fertile offspring MT-71was obtained, and it created new ordinary maize germplasm. The setting percentage of MT-71 was 7.84%(3X/2X)，0.56%（2X/3X），and 0.52%(3X /4X) respectively. The open pollination rate was 1.18% The chromosome configuration of the pollen mother cells (PMC) was 2.30I+3.72Ⅱ+6.49Ⅲ+0.23Ⅳ. The chromosomes of 3X/2X progenies had almost all 2n=20-30 chromosome number, while the chromosome number was only 2n=20, 21 in 2X/3X and open pollination progenies. These results showed that the MT-71 could produce a function male and female gametes, and n=10, 11 gametes were heritable. Meanwhile, the extra chromosome transmission ability of the female gametophyte was significantly higher than the male gametophyte, and 3X/2X was the most effective mode of allotriploid.