Abstract:Abstract：RING(Really Interesting New Gene) ubiquitin ligases play significant roles in rice, such as growth, development，resistance to biotic and abiotic stresses. In this study，we cloned the RING finger gene (OsRING6) from the Japonica cultivar Nipponbare (Oryza sativa) based on the sequence analysis of the rice genome and the RT-PCR method. The OsRING6 full cDNA consists of 1355 bp nucleotides and contains a CDS of 918 bp nucleotides，and encoding a protein of 315 amino acids with the deduced molecular weight of 32.96 kD and a pI value of 3.49. The similarity comparison revealed that the CDS sequence of OsRING6 shared 97% and 83% homology with Oryza sativa Indica (CT832014) and Oryza brachyantha (XM_006659599)，respectively. The relative molecular weight of the MBP-OsRING6 recombinant protein induced by IPTG was about 100 kD，which was much bigger than deduced value of 76 kD. Promoter analysis indicated that it contains several regulatory elements related to drought stress, light response，ABA and GA signal transduction. Real-time PCR experiments showed that OsRING6 was expressed mainly in root，stem，leaves，leaf sheath，flowers and seed，and its expression level was the highest in germinated seeds and the lowest in flowers among these detected tissues. We also found that OsRING6 could be induced when treated with salt and ABA，respectively，and inhibited by PEG. These data suggest that OsRING6 might be involved in abiotic stresses such as slat and drought, and could be used for further functional study and breeding in rice.