Abstract:Multi-allele Genic male sterility is one of the most important methods for hybridization breeding in Chinese cabbage (B. rapa L. ssp. pekinensis). A segregating population F1S4 from a cross between a male sterile line 939A and a fertile line YQD56A which contained restoring gene was developed. Bulked segregate analysis (BSA) was used to screen markers linked to the MS gene. At the same time, an F2 population from a cross of 939A and a fertile line yellow sason which contained maintainer gene was received for the confirmation of the location and fine mapping of MS. Bulked segregate analysis (BSA) was used to screen markers linked to the MS gene. Finally 2 markers, A08_1900 and BrID111035, were found in F1S4 population, linked to MS. The distance was 8.8 cM and 2.5 cM, respectively. In F2 population, the MS were mapped in the same region in chromosome A08 with its in F1S4 population. Further, Br19470306 and Br19675586 were found tightly linked to MS. The distance was 1.6cM and 2.4cM, respectively.In this region, 58 genes were found. These results can be used for finding the candidate gene of MS and then accelerate breeding programs.