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期 刊 ：植物遗传资源学报 2016年 17卷 1期

关键词：榉树；指纹图谱；SRAP标记；毛细管电泳技术；

Keywords:Zelkova schneideriana, fingerprint, SRAP marker, Capillary electrophoresis,

摘 要 ：本研究建立了榉树SRAP分子标记技术体系，并在此基础上结合毛细电泳技术(Capillary Electrophoresis , CE)对来自贵州、江西、浙江和日本京都的70个榉树优良单株进行指纹图谱构建和遗传聚类分析。筛选出13对SRAP引物，共扩增出2276个位点，均为多态性条带，多态百分率达到100%，筛选出的任意一条引物在采用毛细电泳技术的情况下均可将所有样本区分开。70个优良单株间的遗传距离变化范围为0.0078~0.3772，平均值为0.0629；遗传相似系数在0.9218~0.9565之间，平均值为 0.9339，说明种源间遗传距离近，遗传相似性较高。SRAP遗传聚类分析结果显示，榉树的分类依次与其所生长的时间、地理位置以及秋季叶色和叶面光滑程度等性状相关。本研究可以为榉树优良单株无性系的分子鉴定、遗传资源管理及品种保护提供参考依据。

Abstract:In this study, the SRAP reaction system of Zelkova schneideriana was established. Combining capillary electrophoresis technology, fingerprinting and genetic cluster analysis were studied for seventy superior individuals of Zelkova schneideriana collected from Guizhou, Jiangxi, Zhejiang province and Kyoto of Japan. Totally 2276 bands were generated with 13 pairs of SRAP primers, of which all were polymorphic and each pair could separate all samples. Genetic distance among 70 superior individuals was 0.0078～0.3772 with an average value of 0.0629, while genetic similarity coefficient was 0.9218～0.9565 with an average value of 0.9339. This suggests that the genetic distance of zelkovas was close and the genetic similarity was high. The cluster analysis based on SRAP markers revealed that the classification of zelcovas related with their origin time, geographic location and the phenotypes such as autumn color and glossy degree of leaves. This study could be useful in identification, genetic resource management and protection of plant breeders` rights in Z. schneideriana.

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