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期 刊 ：植物遗传资源学报 2014年 15卷 5期

关键词：茶树；SSR；品种鉴别；无性系；育种；

Keywords:Tea, SSR, cultivar identification, clonal, breeding,

摘 要 ：摘要：为促进浙江省茶树育种的发展，本文使用SSR引物研究了浙江省茶树育成品种的遗传多样性，筛选出可用于鉴别浙江省茶树品种的核心鉴定引物和标准品种，并进一步应用于未知茶苗身份鉴定。首先，使用35对SSR引物研究了36个茶树育成品种，并做了聚类分析；然后，根据电泳谱带和基因型筛选出核心鉴定引物和标准品种；最后，对4株未知茶苗进行了身份鉴定。结果表明：共有34对引物表现出多态性，各品种基本按遗传背景聚类，重复样本间遗传距离介于0~0.094；有10对引物确定为核心鉴定引物，8个品种为标准品种；4株未知身份茶苗中，NH-01属于乌牛早品种，另外3株并非浙江现有品种。本研究认为，核心鉴定引物在两个浙江育成品种间差异引物对≥2时，应判定为不同品种；差异引物对≤1时，应判定为相同品种或极相似品种，必要时应引入其余24对引物计算遗传距离进一步验证，遗传距离>0.140判定为不同品种，遗传距离≤0.140判定为同一品种。

Abstract:Abstract: Thirty six tea cultivars origin from Zhejiang province were analyzed using SSR markers. The genetic diversity and cluster analysis shown that: totally 121 alleles and 187 genotypes were amplified by 34 polymorphism markers, with an average of 3.5 and 5.5, respectively; the polymorphism information content (PIC) were ranged from 0.02 to 0.74, average 0.45; most similar cultivars were classified according to their genomic background. Ten core SSR markers fitting for cultivar identification were confirmed, and were used to evaluate 4 unknown varieties. It is believed that if more than 2 core primer pairs were different between 2 tea plants, they should be defined as different varieties; otherwise they are same variety or highly similarity varieties. If necessary, the value of genetic distance should be used to distinguish highly similarity varieties, if the value >0.140 they should be defined as different varieties, otherwise they are same variety. We believe that, this study will promote the breeding research of tea plant in Zhejiang province.

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