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期 刊 ：植物遗传资源学报 2014年 15卷 3期

关键词：浙江；柿树；遗传资源；多样性；RAPD；

Keywords:Persimmons, Germplasm Resources, Genetic Diversity, RAPD,

摘 要 ：浙江柿树资源丰富，但品种或类型混杂，为探究浙江省柿树种质资源多样性，本文利用筛选出的16条随机引物，对7个柿树类型40个单株进行了RAPD分析，共得到条带156条普带，其中多态性条带138条，平均多态位点百分率为87.76%。类型间遗传多样性(Dst)为0.7604，远高于类型内个体间遗传多样性(Hs)为0.0603，各类型分化系数(Gst)为0.7604，基因流(Nm)为0.1576。并根据各类型间的遗传距离，将各类型进行聚类分析。结果表明研究的各类型遗传结构比较一致，而不同类型间分化较为严重，存在较为丰富的遗传多样性，且有一定的的基因交流。今后应收集更全面的柿树种植资源，为柿树种质资源的保存及今后良种杂交及选育打下基础。

Abstract:Persimmon resources are rich in Zhejiang province, but they are complicated between varieties or types. To investigate genetic diversity of Persimmon in Zhejiang, 40 Persimmon trees of 7 types were RAPD using 16 selected random primers. 156 fragments were showed in total, and in which 138 were Polymorphisms fragments. Average percentage of polymorphic loci was 87.76%, genetic diversity index (Dst) between types was 0.7604, outclass that within type (Hs) of 0.0603. The coefficient of differentiation (Gst) was 0.7604, and the gene flow was 0.1576. At last, 7 types of Persimmons were clustered according to the genetic distance between then. the result showed that genetic structure were basically identical within each type, but there were lot of different between 7 types, and some gene communication. Widely collection of Persimmons resources should be researched in future, so as to lay a foundation for the conservation and fine variety breeding of Persimmons

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