摘 要 ：研究甘蔗属与滇蔗茅染色体在属间远缘杂交F1子代中的遗传行为具有重大科学意义，可为发掘利用滇蔗茅野生优异基因资源提供细胞学依据。本研究采用常规染色体压片技术对远缘杂交父本及杂交F1子代花粉母细胞减数分裂过程进行观察，结果父本云南95-19分裂正常，而杂交F1子代存在异常分裂行为；进一步利用基因组荧光原位杂交技术（genome in situ hybridization, GISH）对杂交F1子代花粉母细胞减数分裂过程进行研究，结果表明：滇蔗茅与甘蔗属热带种的亲缘关系较远，这两个属的染色体在减数分裂细胞中不能进行同源配对，15条滇蔗茅染色体以单价体形式存在，减数分裂细胞中存在落后染色体、染色体丢失和不均衡分离现象，因而杂交F1子代花粉完全败育。
Abstract:It is significant for understanding the behavior of chromosome in Saccharum officinarum ? Erianthus rockii F1 hybrids, because it can provide cytology evidence for utilization the wild Erianthus rockii resource. In this paper, the Saccharum officinarum ? Erianthus rockii F1 hybrids and their male parent were used for materials, the meiosis of pollen mother cells were prepared by use squash technique and then were observed under a microscope, the result showed that division of the male parent yunnan95-19 was normal, but in F1 hybrids some abnormal behavior were observed; the further research was proceed by used the GISH technology, and the result indicated that Erianthus rockii has a distance relationship from Saccharum officinarum genus, because in the pollen mother cells of F1 hybrids the chromosome from these two genus could not pairing in meiosis, we detected 15 chromosomes which from Erianthus rockii were formed 15 univalent respectively, further more, the meiosis were observed with lagging chromosomes, elimination chromosomes and disequilibrium separated chromosomes in pollen mother cells of the F1 hybrids, the abnormal division we observed in this paper may illustrate why the pollen were abortive completely in F1 hybrids.