免费文献传递   相关文献

Regulations of berberine on gene expression of BMP4 transcriptional pathways to improve visceral white adipose tissues insulin resistance in type 2 diabetic hamsters

黄连素调节BMP4转录通路基因表达改善2型糖尿病地鼠内脏白色脂肪组织胰岛素抵抗的研究



全 文 :)$#
y
)
z ä
&
åä
4
}
Z-Mc&$ ;-+4 HISNYUN6$ )$#
œš0›œ
C9A&
ž@N¾¿Ÿ WX
)
ß
¡¢ÕFY8£c¤ó]À¥¦§0¨©˜‹Œ
5Ô<

!
!
(&
)
!
!
5ڇ
)
!
T(¸
)
!
I)

!
(|*

c
küQakS êëR4Qm S“a
!
T÷ kü
#$
)c
küQakS êëkü}%‘Qm UÄVa
!
T÷ kü
#$44#

hi
#!
‹Œœš0g
)
ß¡¢ÕFY8£c¤ó]À¥
!Za2F
$
C9A&
ž@Nèc
=
ÄóÀ¥¾@N¾¿
.>;2
Ÿ ˜}7öƒ<ӛf
+
™š”ó)O»¼êš¦§0¨©FYçß
$
:;:Þ%-›×0—˜
)
ß¡¢ÕFYçß
+
@ç#LCËfŠ‰ŠXgÚÀ

ꚦ§0¨©À
)
ß¡¢ÕÀè
)
ß¡¢Õœš0ÂÃÀ
+
ÂÃ
(
uC
$
™š;O´%
A<>
’9ȓxÀFY
Za2F
$
C9A&
@N

c
=
ÄóÀ¥¾@N¥¾¿˜
.>;2
Ÿ W´
+
#Ê&Ò
$
çßFY
Za2F
$
C9A&$C9A>

$C9A>M2$8.UP$8.UP5$8.UP$J4=92AK$2FH)$A>?9#$<=RCA

$AQ<
!
$AA2>
%¥ÄóÀ¥$
þ¿˜
.>;2
Ÿ «ˆ
$
.
8.UP#$8.YNècóÀ¥$þ¿˜
.>;2
Ÿ ·B
+
œš0Âۜ
Za2F
$
C9A&
ž
@NèÄóÀ¥ž@N
$
»¼ÄóÀ¥$þ¿
.>;2
˜Ÿ 
$
»¼c¤ó]Ĥ_¾¿Ÿß
$
WX󻨦§0¨©
+
#ʟ‘
$C9A&
ž@Nüƒó»¨8£c¤ó]À¥¦§0¨©
!H:Za2F:>
˜±‰¥œš0»¼c¤ó]Ĥ_˜Š
¯f
+

jkl
#!
œš0
,)
ß¡¢Õ
,
c¤ó]À¥Ä¤_
,
¦§0¨©
,C9A&,8.UP,J4=92AK

mn)(
#!)$53$53#

rsAt
#!
!
5Ô<
!
ga
!
Ž†KQi
!
†‡ˆ‰WXSSY‹OZ[“Écjt·
!FIM$ $(%%%! R3.ULM$ VY-OWI0VML((1
#4c,-.
!
(&
!
ga
!
Ž†KQi
!
†‡ˆ‰WXS‹\Æ]^_‹OZÉcjt·
!FIM$ 4555(#4)$#!R3.ULM$7YWU0MLY))%%1W-T3
.ULMc,-.
97N>/-1.24:28<73<73.4724N7477E;37::.2428MYA& 13-4:H3.;1.24-/
;-16P-@:12 .,;32J7J.:H73-/P6.17-0.;2:71.::>7:.4:>/.4
37:.:1-4H7.41@;7) 0.-<71.H6-,:173:
B:QY-3OWI0V

!
$ B:D Y`3WU0
)
!
$ B:` L036Y
)
$ Q2G[WI0V30U0
)
$ @D2;QBU0

$ B:D U^3ML

!cN)G#*,;)$,./E#,4.2.%-$ ,4)P*+,:/2#,)1 C.+G,#2./N#2#$ 5)16#2$()*+,-$ N#2#$ #$$ 34$#,
)cN)G#*,;)$,./?$1.6*$.2.%-$ N#2#$ 59$6G#23)$,*#2C.+G,#2:/2#,)1 ,.N#2#$ 5)16#2$()*+,-$ N#2#$ #$44$ 34$#
-B<:13-H1.!F-OTYP6TWII\I,TO-\SINSINL0I-0 TWIVI0I.>;2I7JNIOOL-0O-\C9A& TNU0O,NLJTL-0UMJUTW*U6OU0P SN-*0=*WLTIUPL3
J-OITLOOYI,-0dINOL-0 TNU0O,NLJTL-0UMJUTW*U6OL0 dLO,INUM*WLTIUPLJ-OITLOOYIO!Za2F L0 T6JI) PLUSITL,WU.OTINOU0P I7JM-NITWI
NIMIdU0T.I,WU0LO.OcFWI-SIOIL0OYML03NIOLOTU0TWU.OTIN.-PIM*INIL0PY,IP S6YOL0VWLVW3\UTPLIT$ U0P TWI0 TWIT6JI) PLUSITL,WU.3
OTIN.-PIM*UO,NIUTIP TWN-YVW L0/I,TL-0 *LTW M-*3P-OIOTNIJT-]-T-,L0 L0 TWI-SIOIL0OYML03NIOLOTU0TWU.OTIN.-PIMc2\TINTWI.-PIML0V$
TWIWU.OTINO*ININU0P-.M6PLdLPIP L0T-0-N.UM,-0TN-M$ -SIOIL0OYML03NIOLOTU0T$ T6JI) PLUSITL,U0P SINSINL0I3TNIUTIP PLUSITL,VN-YJOc
2\TINTWI0L0I3*II_ TNIUT.I0T$NIUM3TL.IbYU0TLTUTLdIA<>*UOYOIP T-.IUOYNITWI,WU0VIOL0 VI0I.>;2I7JNIOOL-0O-\Za2FC9A&
TNU0O,NLJTL-0UMJUTW*U6O$ SN-*0=*WLTIUPLJ-OITLOOYI,-0dINOL-0 TNU0O,NLJTL-0UMJUTW*U6OU0P TWILNTUNVITVI0IOL0 PL\INI0TVN-YJOcFWI
NIOYMTOOW-*IP TWUTTWIVI0I.>;2I7JNIOOL-0O-\C9A&$ C9A>

$ C9A>M2$ 8.UP$ 8.UP5$ 8.UP$ J4 =92AK$ 2FH)$
A>?9#$ <=RCA

$ AQ<
!
$ AA2>
%
U0P SN-*0 UPLJ-OITLOOYI3OJI,L\L,VI0IO*UOPI,NIUOIP U0P TWUT-\8.UP#$ 8.YN\ U0P
*WLTIUPLJ-OITLOOYI3OJI,L\L,VI0IO*UOL0,NIUOIP L0 Za2F-\.-PIMWU.OTINOcFNIUT.I0T*LTW SINSINL0INIVYMUTIP C9A& TNU03
/&5/
•%Øó
#
œš0›œ
C9A&
ž@N¾¿Ÿ WX
)
ß¡¢ÕFY8£c¤ó]À¥¦§0¨©˜‹Œ
O,NLJTL-0UMJUTW*U6OU0P SN-*0 UPLJ-OITLOOYITNU0O,NLJTL-0UMJUTW*U6OU0P L0PY,IP TWIVI0I.>;2I7JNIOOL-0 -\SN-*0 UPLJ-OI
TLOOYI3OJI,L\L,VI0IOL0 Za2FT-PIdIM-J SN-*0L0VVI0IJWI0-T6JI-\*WLTIUPLJ-OITLOOYIO$ U0P TWI0 L.JN-dIP \UT3L0PY,IP L0OY3
ML0 NIOLOTU0,IcFWIOI\L0PL0VOL0PL,UTIP TWUTC9A& TNU0O,NLJTL-0UMJUTW*U6OL0d-MdIP L0 TWI\-N.UTL-0 -\\UT3L0PY,IP dLO,INUM*WLTI
UPLJ-OITLOOYIOL0OYML0 NIOLOTU0,I!H:Za2F:> U0P TWISN-*0L0V.-MI,YMUN.I,WU0LO.-\*WLTIUPLJ-OITLOOYIOL0PY,IP S6SIN3
SINL0Ic
-[7@ P230:.!SINSINL0I, T6JI) PLUSITIO, SN-*0L0V-\*WLTIUPLJ-OITLOOYIO, L0OYML0 NIOLOTU0,I, C9A&, 8.UP, J4=92AK
02.#!( %^)_FHSH,,(!)(&)
!!
ó]À¥Ãróþˆèó]À¥c¤_@‰˜
󻨦§0¨©þ”ó)O¨êšmØ
)
ß¡¢
՘¡Zf
-34.
+
ëyF
$
»¼c¤ó]Ĥ_þ
zÂ)O¨êš¥Ó›
)
ß¡¢Õ˜„â/
-&.
+
œš0þ!%W$È,œš˜ÏZ؏­¨
‰Š
$
g¡¢Õ¥ömSB&˜ÃÇöŸ™R
z˜7ø¨
-5.
+
%닌&Ҝš0#BĤ
ó]Ÿß˜»¼Ç™
-#.
$
Egœš0»¼
Za2F
Ĥ_¾¿Ÿß.ÂÃ
H:Za2F:>
˜Š¯f
AøIl
+
¿1
$
[‹Œ—˜êšè¡¢ÕFY
çßö‹Œœš0g¡¢ÕFY
Za2F
$
C9A&
ž@Nӛ¾¿
.>;2
Ÿ ˜}7
$
ƒ<½@
̛œ
C9A&
ž@N»¼
Za2F
Ĥ_˜Š
¯f
+
!O
¦Ë
;O3î´%
A<>
]
!
½%
CL-3NUP
DE
,
Ù
œ
G0I3F-Y,W
t¡]
!
½%×ØDE
,

$#$
w
6>Ø_ŠÄ]
!
{[{˜DE
+
ÉÆLá
!FQ
_`ó]a
!HH2O
ˆ‰Šó
zc
!B?B
”‰Šózc
!@?B
uÕ2?
!F<
ȓ.Þ±¨6
>U0P-7
DE
,
Y¦§0

0èó
,0
RB:82
.Þ±¨6½%
BL0,-
DE
,>;2
ö÷
.Þ±
!>;IUO69L0LKLT
èXž.Þ±
!G.0LO3
,NLJT>IdINOIFNU0O,NLJTL-0 KLT
¨6Ù%
ELUVI0
D
E
,@-T8TUNFUbF9?;2
ŒKá¨6
FUKU>U
DE
,
>;UOIL0WLSLT-N
è
GMLV-3PF
¨6½%
AN-.IU
DE
,
;O3î´%
A<>
ڙ.ޱ
!LE8 C^>QNII0
A<>KLT
¨6½%
CL-3NUP
DE
,
-›×0
!8F[
è
œš0¨6
8LV.U
DE
,
½½Xš.Þ¨623à
%؏()DE
+
#$
oy©ØÈU$%FY
-
¸oÂA&î
;Ê>‹Œe
$8<` K!
o
)$$&)#.$)#
uA
$
|
éxå
$
™žäË
$
ä˜Ý@äË
$

!&c u
$c&) ..-M/B
j
$
º¡
!)4c$ u(c% V+
O
’9
!`O
>ç߁4

ŠÀ¥ÂÃ
!
ÙÚ2«˜@ç
’9—˜FYçß
-%.
+
ÈUFY
#$
o
$
|éxå
$
ËfŠûÝOŠXgÚÀè”ó)OÀ
$
í¾
$
o
$
ä˜Ý@äË
,

5$
o
$
䘔ó)O
+
äË
&
uC
$
”ó)Oå˘FY6m±‰êš¨¦§
0¨©çß
$
Rmeꚨ¦§0¨©FYËfŠ
‰
)
À
$

!&$
oFY

‡O
) W
C
$
Ù
&$ .V/
_V
j
!
R
8F[
š
$c ..-M/B
j
$ J@&c)
˜qן
 aì!ÿµ‰
k 8F[
5ÿ
$$c) ..
{ñÌN


˜Þ%ìÅ
) P )
3ÃPýþ
8F[$
ýþCK
á‡O
) W
—˜
)
ß¡¢Õçß
+
k[À
!$
o

¥ÝOŠXgÚÀ
!$
o

ýþó%˜
$c ..-M/
B
j
$ J@&c)
˜qן aì!ÿ
!
ýþ’èèÞ
%Ó·
+
i—C
$
xÀFYKá/àÖ)O
)
u
+
¡¢Õ覧0¨©FYO²ëÃt¡

ëæ§
0èÐ|ëì¡7%.Ê2´
+
·OoØ\N
4
ÿ
‘S
#
/¦§0¼-ä=«ˆ
$
›¦§0˜¼-¨
«ˆ
,
0Ð|ëì¡7%;Êëì¡ÇÛ\8
ˆ%
)#,
1
%) W
CëÃt¡%
( ..-M/B
j
û
)
ß¡¢ÕçßFY
+
@ç#LOÁ¹9
)
ß¡
¢ÕFY
)(
o
+
@çɉC
$
FYŠû
&
À
#
g
ÚÀ

¦§0¨©À
)
ß¡¢ÕÀ蜚0ÂÃ
˜
)
ß¡¢ÕÀ
$

$
o
+
œš0ÂÃÀÙ
|o|5Ä|
5$ .V/ _V
j
œš0
!
ôŠ%
(5k$
Ѻޜš0
$
5ï-Á¾./0
=AC8
5
ÿ

˜Þ%ÂÃ
(
u
+
gÚÀ

¦§0¨©Àè
)
ß¡¢ÕÀ|oÄ|әÞ%˜-Á¾./0
=
AC8
5ÿ
+
ÂÃ}ìgÚÀå˜Ý@äË
$
½½
xÀKá嘔óäË
+
;Ê}ìbmØ>
km™v
+
;Ê#LO
$
xÀFY”‡O
(c5 W
OÈ¡·O:,
$
”:,
)c5 W
O
$
¶÷tÿ‘[
öik
$
Š`
Za2F!
íîÓ,Øó]À¥

IR
€u:ó]¡èU¢££¯¬£ó]À¥
$
È
/55/
)$#
y
)
z ä
&
åä
4
}
Z-Mc&$ ;-+4 HISNYUN6$ )$#
¡
$
ð1Šó]ÿFäÀöRîï
j$ n

$
l1Šó]2´ï
$k
$¨Áû5ÿ
+
`O
Ð|ëì¡7%.Ê
!GQFF!
‡O
) W
C
$
:;ëì¡
) V/_V
j
Ē
$
”ù´˜OìÑ
!$$4$$
#$$)$$$ .L0
“t¡
+
¡7%ÇÛ\8ˆ
p=)
ëÓ´d
f#$ .L0
“´d
f)$ .L0
“´d
f=)
$ .L0
“´d
+
&`O
tØ_ȓ
!
™š{˜
$#$
w6>Ø_Š
Ä]ȓt«
HH2O$F<$B?B$@?B$FQ
˜³Š
+
™šÙœt¡]ȓt¡
+
™š
BL0,-
DE
RB:82
.ޱȓt«¦§0

0èó,0
³Š
+
%`O
±Û¯M
!Za2F
2´ï
$k
$¨Áû5
ÿ
$

$
í¤
$
*
$@R
¤¤
$
îC¯M
+
#`O
;O´%
A<>
ȓ
!
÷Àîó]À¥
4$ .V$
Ù
>;IUO69L0LKLT
m‘rö÷u
>;2+
ȓ
>;2
³Š

ôŠö³´ÉQ¨
+
;O3î´%
A<>
Ù
LE
8 C^>QNII0 9L7KLT
m‘rW‚
+
—˜
5
.
B
˜Ú
™º,
+
ڙ°[þ
#(5 n 4 .L0$ (5 n $ O$5
n &5 O$&5
HÞM
+
¾·(C
$
f5øÇÛȓž
˜¦[¨
+
|Hڙ¦¡q
4
3
+
ù˜÷¨g
Ú
+
Vȓ
$
|gQ
!
Ÿ

˜¾·Çc¦-ëï
+
š
)
j
66
3
,
’9gÌn[쑾¿Óg¯%
+

3
U,TL0
û8ü¾¿
+
)`O
gŠÄ
!
;Ê=²˜ $
&u+
ŸÒ
+
g
ÂϚ
8A88 %c$
n
,
ÈÊ虿0’ŠÄ
ÈÊ
+ E
#
$c$5
ûÂÃBgd]
+
&O

& !`O
¦§0¨©À
)
ß¡¢ÕÀ蜚0ÂÃÀ
FY˜Pb$¼
!
xÀFY˜ŸßèPb$¼Ÿ
)+
;Ê#LO
$
ƒgÚÀÓ&
$
çßFYº¡&
·B
!Eq$c$5$
Ÿ™êš
+
çßFY˜
Za2F
¡
%‘&·B
!Eq$c$5$Za2F
¡
=
º¡&”ïg
ÚÀ
!Eq$c$5+
ÓA
@?B
ÙÚ«ˆ˜í
$
çßF
Y˜tó
!
íî
HH2O$F<$B?B
è
FQ
ÙÚ&”
!Eq$c$5+ )
ß¡¢ÕFY˜t¡&\”
!Eq$c$5+
¦§0¨©FYŸ™‘&˜”¦§0
tS
$
.
)
ß¡¢ÕFY˜¦§0ÙڃgÚÀÓ
Â
+
çßFYt«ó,0«ˆ.0·B
!Eq
$c$5+
çßFY
GQFF
xä´Ñ˜t¡d·”
!Eq$c$5$
t«¦§0˜ÙÚÃX
$GQFF
˜¡Ç
Û\8ˆ·ð
!Eq$c$5$
¦§0¨©FY˜¦
!!!!
Ÿ
!
;O3î´%
A<>

FUSMI!ANL.INOYOIP \-NNIUM3TL.I>F3A<>
QÀÈ ÝN< QÝN
!5i34i
C9A& ;9g$$%55& 2Q<<2F<2Q<2FFC9A>

;9g$$%5# 222Q<2QQFQ2QFQC9A>2 ;9g$$(%5 8.UP ;9g$$54( 2Q2<8.UP5 ;9g$$5& Q2Q8.UP 2H%5&$ <2Q<J4=92AK ;9g$(5 <2QFF<22<<2FH) ;9g$$$)5$(4 <2FQQ<2QFQQ2FFQQFF2Q
QQ2QFFQFQFQ2Q8.UP# ;9g$$5&) Q<2QFQ2FQ2QQQ2QFFQQFQ
8.YN\ ;9g$$$4#)% 2F2222QQQH28 ;9$$%( <2<2Q2FQ2FQ2<2QQ2Q2FQQ
F8Q2<< 2H45#$( 2<2<2<2<22A>?9# ;9g$)%5$& QF<<=RCA

;9g$$(4 QFFF2<2QFF2<2AQ<
!
C<$### FQ<22Q2QQQAA2>
%
;9g$&# <<<Q<22F<22F2Q22QQ22<2D2QRM-dM4 ;9g$$%%$4 F2QF<<2QQ!
;9g$$)%5)4 2F<<22Q2F<2FQ222FQ;N\ ;9g$$#&))# 22<<2QQFQ2F\U. ;9g$$(4#$ <<2FQFQQ<QQ<
3U,TL0 2^ #5#( 2Q2QQQ222F<22F2QFQ2FQ2</#5/
•%Øó
#
œš0›œ
C9A&
ž@N¾¿Ÿ WX
)
ß¡¢ÕFY8£c¤ó]À¥¦§0¨©˜‹Œ
§0ÇÛ\8ˆ·ð
!Eq$c$5$
.
)
ß¡¢ÕF
Y˜¦§0ÇÛ\8ˆƒgÚÀFYÓëÂ
!
q
+
çßFY˜¦§0¼-ä=«ˆ
!Eq$c$5$
.¦§0¨©ä=\”
!Eq$c$5!
Ÿ
)+
V̜
š0ÂÃC
$
ƒ¡¢ÕFYÓ&
$
œš0ÂÃÀFY
˜
Za2F
!%‘&«ˆ
!Eq$c$5+
ÓA
@?B
Ù
Ú·”˜í
$
ÂÃÀFY˜tó
!HH2O$F<$B?B
è
FQ
ÙÚèt¡³Š&«ˆ
!Eq$c$5+
ÂÃÀ
FYt«ó,0·B.0«ˆ
+
ÂÃÀFY˜
GQFF
xä´Ñ˜t¡³Š·ˆ
!Eq$c$5$
xä´
Ñt«¦§0˜ŠíèŠíÙژëï™qŠX
+
ÂÃÀFY¡ÇÛ\8ˆ·l
!Eq$c$5 !
q
+
ÂÃÀFY¦§0¼-ä=·ð.¦§0¨©ä=
·:
!Eq$c$5+
Ÿ
)!
gÚÀ

¦§0¨©À
)
ß¡¢ÕÀ蜚0ÂÃÀFY˜PbŸß$¼
!
$
&u+$$p$
FUSMI)!9ITUS-ML,,WUNU,TINL]UTL-0 -\WU.OTINOL0 ,-0TN-M$ L0OYML03NIOLOTU0T$ T6JI) PLUSITL,U0P SINSINL0I3TNIUTIP T6JI) PLUSITL,
VN-YJO!
$
&u+$$p$
Àê
º¡
=V
8£ó]!%
=V
8£ó]!%º¡
=k

=..-M/B
j
t¦§0
=
.
V/B
j
ŠXgÚ
)&c uc 4c& u$c% )c%$ u$c& &c)# u$c5 $c( u$c$
¦§0¨© &c u(c5

#c5 u$c(

&c4( u$c

#c&) u$c(4

c# u$c)

)
ß¡¢Õ &)c% u%c%

#c$ u$c

&c$ u$c#

)c$ uc4

c$( u$c)
œš0Âà 4c5 u#c)

&c u$c%
$)
4c&$ u$c&
$)
5c( uc$&
$)
c$% u$c)$
Àê
ÉÆLá
=..-M/B
j
_`ó]a
=..-M/B
j
ˆ‰Šózc
=..-M/B
j
”‰Šózc
=..-M/B
j
uÕ2?
=..-M/B
j
ŠXgÚ
)c5 u$c5$ c( u$c&% )c5$ u$c5 c#) u$c( %c$) u$c5%
¦§0¨© c uc54

c4# uc$5

)c uc5&

$c( u$c4

#c#5 u)c$&

)
ß¡¢Õ c( uc%5

c#$ uc)$

4c(4 u)c4$

$c(4 u$c)$

%c$ u)c)%

œš0Âà 5c$ u$c$
$)
)c%$ u$c()
$)
&c%4 uc((
$)
c)( u$c))
$)
c# uc&
$)
Àê
tó,0
=.V/B
j
t0
=
.
V/B
j
D
$
t8
$
:>
@G92
ED:ŠXgÚ
4c$ u%c)% #c(4 u$c4 $%c$$ uc%) &c5$ u$c(& $c4$ u$c$$%
¦§0¨© (c(5 uc$

c%( uc5

)$c%& u&&c5%

)c4$ uc%

$c)%4 $ u$c$$&

)
ß¡¢Õ c4# uc(&

)c#% uc#)

44$c5& u&c

5c$$ u)c4$

$c)5( ( u$c$$5

œš0Âà )5c# u)c5
$)
(c&( uc%%
$)
%c4( u44c&4
$)
%c#$ uc#$
$)
$c)# ( u$c$$(
$)
!!
ý
#ED:$
fMVD
$
$8
$
ûëæ§0
$
™r
.D/B
j
$Q
$
ûëÃt¡
$
™r
.V/ PB
j
,@G923:>
£
:>
@G92
p
ëÃt¡
!..-M/B
j
t
ëæ§0
!.D/B
j
=))c5,D
$
t8
$
!D
$
™r
..-M/B
j
$8
$
™r
.D/B
j
,
ƒgÚÀ&

Eq$c$5, )
ß¡¢ÕÀƒÂÃ
À&
)
Eq$c$5+
& `O
¦§0¨©À
)
ß¡¢ÕÀ蜚0ÂÃÀ
FY
Za2F
±ÛW´
!
ƒgÚÀ&
$
çßFY
!
¦§0¨©FYè
)
ß¡¢ÕFY

˜
Za2F
ó
]jkºˆ·ð
$
ðó]jk=%·‹
+
œš0Â
ÃCçßFY˜
Za2F
ó]jkºˆ·:
$
ðó]
jk=%«ˆ
!
q
)+
& &`O
¦§0¨©À
)
ß¡¢ÕÀ蜚0ÂÃÀ
FY
Za2F
$
C9A&
ž@N

c
=
ÄóÀ¥¾
@N¥Ó™¾¿˜¾¿Ÿ W´
!
;O´%
>F3
A<>
˜#Ê&҃gÚÀÓ&
$
çßFY
!
¦§0
¨©FYè
)
ß¡¢ÕFY
Za2F
$
C9A&$C93
A>

$C9A>M2$ 8.UP$ 8.UP5$ 8.UP$ J4=92AK$
2FH)$A>?9#$<=RCA

$AQ<
!
$AA2>
%¥ÄóÀ
¥$þ¿Ÿ
D!
$¥Ûãº
ï_ӛ¾¿Ÿ
;N\$F\U.
è
˜¾¿
.>;2
Ÿ «ˆ
$
.
8.UP#$8.YNèc¤ó]$þ¿Ÿ
ó]aK‰á
!H28
=>Àá
2
-_á
!2<<
è
\ó>Àá
2
¢.èá
3!8˜¾¿
.>;2
Ÿ
 ·B
+ C9A&
ž@N

c
=
ÄóÀ¥¾@N¥
ә\_¾¿˜¾¿Ÿ W´”¦§0¨©FY
è
)
ß¡¢ÕFY(ìî”&ÂÃ
!Eq$c$5+
œš0ÂÃC
$
ƒgÚÀÓ&
$
ÂÃÀFY
Za2F
$
C9A&$C9A>

$C9A>M2$8.UP$8.UP5$8.UP$
J4=92AK$2FH)$A>?9#$ <=RCA

$AQ<
!
$AA2>
%
/%5/
)$#
y
)
z ä
&
åä
4
}
Z-Mc&$ ;-+4 HISNYUN6$ )$#
!!!
ƒgÚÀ&
$
!
Eq$c$5,)
ß¡¢ÕÀƒÂÃÀ&
$
|
Eq$c$5,2c
ëì¡ÇÛ
,Cc
¦§0ÇÛ
,ëì¡ÇÛ\8ˆ
,?c
¦§0ÇÛ\8ˆ
+
q
!
gÚÀ

¦§0¨©À
)
ß¡¢ÕÀ蜚0ÂÃÀFYÐ|¡7%.Ê
HLVc!GNUMVMY,-OIT-MINU0,ITIOTO!GQFF -\WU.OTINOL0 ,-0TN-M$ L0OYML0 NIOLOTU0T$ T6JI) PLUSITL,U0P SINSINL0I3TNIUTIP T6JI) PLU3
SITL,VN-YJO
2c
gÚÀ
,Cc
¦§0¨©À
,ß¡¢ÕÀ
,?c
œš0ÂÃÀ
+
q
)!
gÚÀ

¦§0¨©À
)
ß¡¢ÕÀè
)
ß¡¢Õœ
š0ÂÃÀFY8£c¤ó]À¥˜óþˆ
!@R$t)$$
HLVc)!BLJLP U,,Y.YMUTL-0 L0 dLO,INUM*WLTIUPLJ-OITLOOYI-WU.OTINOL0 ,-0TN-M$ L0OYML0 NIOLOTU0T$ T6JI) PLUSITL,U0P SIN3
SINL0I3TNIUTIP T6JI) PLUSITL,VN-YJO!@R$t)$$
¥ÄóÀ¥$þ¿Ÿ
D!
$¥Ûãºï_ӛ¾¿Ÿ
;N\$F\U.$˜¾¿
.>;2
Ÿ ·B
$
.c¤ó]$þ¿
H28$2<<$
8˜¾¿Ÿ 5·B
+ 8.UP#
è
8.YN˜¾¿
.>;2
Ÿ «ˆ
+

)
ß¡¢ÕÀFYÓ&
$
ÂÃÀFY
Za2F
$
C9A&$C9A>

$C9A>M2$
8.UP$8.UP5$8.UP$J4=92AK$2FH)$A>?9#$
<=RCA

$AQ<
!
$AA2>
%¥ÄóÀ¥$þ¿Ÿ
D!
$¥Ûãºï_ӛ¾¿
Ÿ
;N\$F\U.
è
˜¾¿
.>;2
Ÿ ·B
$
.
8.UP#$8.YN\$H28$2<<
è
8˜¾¿
.>;2
Ÿ
 «ˆ
+ C9A&
ž@N

c
=
ÄóÀ¥¾@N¥
ә\_¾¿˜¾¿Ÿ W´”œš0ÂÃÀè
)
ß¡¢ÕÀ(ìî”&ÂÃ
!Eq$c$5!
q
4+
%O

ꚦ§0¨©þ»m
)
ß¡¢Õ˜¡Z.X
˜M9¿0
$
þ
)
ß¡¢Õmؘ¾èí}Õ
/5/
•%Øó
#
œš0›œ
C9A&
ž@N¾¿Ÿ WX
)
ß¡¢ÕFY8£c¤ó]À¥¦§0¨©˜‹Œ
!!
ƒ¦§0¨©À&
!
Eq$c$5,)
ß¡¢ÕÀƒÂÃÀ&
|
Eq$c$5+
q
4!
¦§0¨©À
)
ß¡¢ÕÀ¥œš0ÂÃÀFY8£c¤ó]À¥
C9A&
ž@N¥c
=
ÄóÀ¥¾@N¾¿ÂÃ
Ÿ ˜Óg´%ŠÄ
HLVc4!>IMUTLdIbYU0TL\L,UTL-0 -\PL\INI0TLUM6I7JNIOOIP VI0IO-\Za2FC9A& TNU0O,NLJTL-0UMJUTW*U6OU0P SN-*0=*WLTIUPLJ-OITLO3
OYI,-0dINOL-0 TNU0O,NLJTL-0UMJUTW*U6OL0 WU.OTINOL0 L0OYML03NIOLOTU0T$ T6JI) PLUSITL,U0P SINSINL0I3TNIUTIP T6JI) PLUSITL,VN-YJO
´
+
[‹Œ$
)
ß¡¢ÕFYçßþGꚦ§0
¨©FYýþ:Þ%-›×0±‰˜
$
öÒG¦§
ºtP¥ÞÛ
!
”¦§0tS

´û¦§ºtL
P¥ÞÛ
!
¦§0ÓgÝØ
$
ßtþ:Þ%-›×
0K瘶Qә&Ÿ¦§ºt˜#Ê
$
æ.çÒ
A
)
ß¡¢ÕmÕÌ͘Pb´_
+
¿1
$
ꚦ
§0¨©ÞÛó]À¥˜Pb$¼è¾¿W´˜‹
ŒBÁïêšÓ›
)
ß¡¢ÕÕ¿‹Œ
$
BÁï
)
ß¡¢Õ,Âþ¿W´˜‹Œ
+

$
»¼c
¤ó]Ĥ_þzÂ)O¨êš¥Ó›
)
ß¡¢Õ
˜„â/
+
œš0‚ûĤó]˜»¼Þ»¼c¤
ó]Ĥ_¾¿Ÿß
!
c¤ó]Ĥ_
$
WX
H:Z3
a2F:>$
·×¦§0‚š
$
‰û
)
ß¡¢Õ,ÂÃ
˜„¯Ñ
-&3#.
+
[‹Œ&Ò
C9A&
ž@N¥c
=
Ä
óÀ¥¾@Nüƒœš0»¼8£c¤ó]Ĥ
_.WX
)
ß¡¢ÕFY
H:Za2F:>
˜Š¯f
+
C9A&
v<@NÏZG
C9A>M2!


è
C93
A>

!
ß

aØ|ßa
3
àßa¿áøºp¼
$
C9A&
#K
C9A>
C
$
)a_
C9A>M2
.½­
_
$
­_˜
C9A>M2
ᯬ
8.UP
v<@Nè
J4=
92AK
v<@N
-3(.
+
½$
$8.UP
v<@NûV®
˜jk8v<@N
$
­_˜
C9A>M2
)a_øº›
ϧ
8.UP!8.UPM$8.UP5
è
8.UP$
7CƒÁµº
ß
8.UP!8.UP&
#K±‰­¨˜žqK
$
í
jk¿
$
›Æ옾¿ž
+
‡ß
8.UP!8.UP#
߃
C9A>M2
#K
$
ÍÎ
8.UPM=5=
˜)a_è
žqK˜±‰
+ 8.YN\M
ß6-#K
8.UPM=5=
ö
p¼½«ø
$
lß#K
8.UP#
«ø
C9A>M2
-$.
+
”
ó]±‰ÌÍ$
$C9A&
ÏZ@Ì
8.UP
v<@N»
¼ÍjkŠ_‰íójk
$
.@Ì
J4=92AK32FH)
v<@N¿­
A>?9#
è
AQ<
!˜ž»¼c¤
£Ä¤íójkŸ Ä¤ó]¾¿Ÿß
-3).
+
#Ê&҃gÚÀÓ&
$
çßFY
!
¦§0¨
©FYè
)
ß¡¢ÕFY
Za2F
$
C9A&$C9A>

$ C9A>M2$ 8.UP$ 8.UP5$ 8.UP$ J4=92AK$
2FH)$A>?9#$<=RCA

$AQ<
!
$AA2>
%¥ÄóÀ
¥$þ¿Ÿ
D
¥Ûã
ºï_ӛ¾¿Ÿ
;N\$F\U.
è
˜¾¿Ÿ «
ˆ
$
.
8.UP#$8.YNèc¤ó]$þ¿Ÿ
H28$
2<<
è
8˜¾¿Ÿ ·B
+
m&ҔçßF
YºtÃX˜
Za2F
$
$
”ó)O‡
C9A&
ž
@N¥½¾¿˜Ÿ 
$
«ˆŸ ˜
8.UP
è
J4=
92AK32FH)
Ójó]jk˜Š_ÚV
$
ÝtBÇ»
¼Ä¤ó]jk–Õ¨Ÿ ›b¾¿
A>?9#
è
/(5/
)$#
y
)
z ä
&
åä
4
}
Z-Mc&$ ;-+4 HISNYUN6$ )$#
AQ<
!˜Ÿ 
$
.¼—c¤ó]jk–Õ¨Ÿ ¾
¿
H28$2<<
è
8˜Ÿ 
$
.Óaž¯Òt
ö·×‰ó
$
¼—£·B
Za2F
óþˆ
$
BÁïó
»¨ó]À¥c¤_±‰
+
·O
$
·B˜óþˆß
@‰Û㺺t¥ó]a
3
ï_ÃX
-4.
+
%_·
B
Za2F
óþˆ
$
tóPbÃX
$
BÁﱉ
H:Z3
a2F:>$Za2F
óif
$
Óa
Za2F
ºt
$
Ÿ™t«
ó,0«ˆ.0·B
+
ÂÃC
$
œš0·BA¡¢ÕÞÛ\«ˆŸ 
˜
Za2FC9A&$C9A>

$C9A>M2$8.UP$8.UP5$
8.UP$J4=92AK
è
2FH)
˜Ÿ 
$
.«ˆ
8.UP#
è
8.YN˜Ÿ 
$
Ÿ™
C9A&
ž@N»¼Ç™
$
BÁïWXó]jk˜Š_ÚV
+
·×˜
J4=
92AK32FH)
v<@NBÁï·B«ˆŸ ˜
A>?9#$<=RCA

$AQ<
!
$AA2>
%
$D!
$è
˜¾¿Ÿ 
$
»¼
A
Za2F
Ĥ_¾¿Ÿß
$
·×Û㺺t
$
»¼
ž¯ÒtǙ
+
¿1
$
œš0»¼˜c¤ó]Ĥ
_BÁï·ò
Za2F
óþˆöWX
H:Za2F:>$
W
Xt¡tó
$
WX
Za2F
ºt
$
Ÿ™t«ó,0·
B.0«ˆ
+
7.
$
#Êx&Ò
$
ƒgÚÓ&
$
œ
š0ÂÃC
$
c¤ó]jk–Õ¨Ÿ ¾¿
H28$
2<<
è
8˜Ÿ êiï\”ÞÛ
$
mþiþœ
š0WXLP¥˜
Za2F
ºtC.Ÿ™·×òó
ºtê[[½‹Œ
$
m£Pƒ±ÛbŸ™‘&
˜Ä¤ó]±Ûӛ
+
»÷eë
$
·q˜
C9A&
ž@N¼—c¤ó
]jk–Õ¨¾¿Ÿ .BÁï
)
ß¡¢Õ
H:Z3
a2F:>
˜±‰
+
œš0Âû¼
C9A&
ž@N
$
»¼Ä¤ó]jk–Õ¨¾¿Ÿ 
$
;™8£c¤
ó]À¥˜Ä¤_
$
BÁïWX
H:Za2F:>
ö™q
Za2F
˜òóóºt
+
¿1
$C9A&
ž@NB¼
‰ûœš0,ÂÃ
)
ß¡¢Õ˜Š¯Ñ
+

uv?w
#
- .!D0VIN>@$ GN,LBcBLJ-T-7L,PLOIUOIO-\0-0UPLJ-OITLOOYIOL0
-SIOLT6-X.c:0TXGSIO>IMUT9ITUS ?LO-NP$ )$$$$ )& ! 8YJJM
)c
- ) .!@UOMU.?a$ XU.IOa AcGSIOLT6-X.cBU0,IT$ )$$5$ 4##
!(&()#(%c
- 4 .!K-JIM.U0 AQcGSIOLT6UOU.IPL,UMJN-SMI. -X.c;UTYNI$
)$$$$ &$&!#%%##45c
- & .!9-LOU0 2$BII^ K$[WU0VX?$ITUMcaWLTI3T-3SN-*0 .ITUS-ML,
,-0dINOL-0 -\WY.U0 UPLJ-,6TIOS6X2KL0WLSLTL-0 -X.c;UTCL-M$ )$5$ % !#5%c
- 5 .!
‰e>
$

c
œš0ÂÃ
)
ß¡¢Õ‹ŒŽ
-X.c
$%
$,-.
$)$&$4(!#4%&c
- # .![WU0V[$ [WU0V@$ BLC$ ITUMcCINSINL0IU,TLdUTIOTWIN.-VI0I3
OLOL0 *WLTIU0P SN-*0 UPLJ-OITLOOYI-X.c;UT<-..Y0$ )$&$
)5!5#5&(4c
- % .!
•%Ø
$
œ¦§
$
\
$
ó
c
-›´×0è”ó)O»¼
)
ß
¡¢Õ$%FYçߘ—˜èÙ´¨¯M
-X.c
$%&A
-.
$ )$$%$ % !(#&(%c
- .!?IN60,_ >$ [WU0V^ Rc8.UP3PIJI0PI0TU0P 8.UP3L0PIJI0PI0T
JUTW*U6OL0 FQH3SITU\U.LM6OLV0UML0V-X.c;UTYNI$ )$$4$ &)5
!#(5#5%%c
- ( .!BL0 QB$ @U0_I0O-0 K?c:0TIVNUTL-0 -\C9A$ a0T$ U0P 0-T,W
OLV0UML0VJUTW*U6OL0 -OTI-SMUOTPL\INI0TLUTL-0-X.cX,WI.$ )$$ )!)#4&(c
-$.!CNUVP-0 C$ 9-OI6,WY_ G$ 8UMPU0WU8$ ITUcC-0I.-NJW-VI3
0ITL,JN-TIL0O#U,NLTL,UMNIdLI*-X.c#$(c
-.!ELU0 8 a$ FU0V^ $ BL` $ ITUMcC9A&3.IPLUTIP SN-*0 \UT3ML_I
,WU0VIOL0 *WLTIUPLJ-OITLOOYIUMTINVMY,-OIU0P I0INV6W-.I-3
OTUOLO-X.cAN-,;UTM2,UP 8,LD82$ )$4$ $!(#R%(c
-).! Y`I>$ aU0 $^ [WU0V8$ ITUMc>-MI-\S-0I.-NJW-VI0ITL,
JN-TIL0 & L0 TWIPL\INI0TLUTL-0 -\SN-*0 \UT3ML_IUPLJ-,6TIO-X.c
2.XAW6OL-MR0P-,NL0-M9ITUS$ )$&$ 4$#!&#R4#4c
-4.!BIML-T<$ ZLPUM3AYLV2XcBLJ-T-7L,LT6$ U0 L.SUMU0,ISIT*II0
MLJ-VI0IOLO1)$.(.U0P \UT6U,LP -7LPUTL-0 -X.c:0TXGSIO>IMUT
9ITUS ?LO-NP$ )$$&$ )!8YJJM))c

xy-0
!
‰ŠŠ
#
/$)5/