免费文献传递   相关文献

Molecular action mechanism of desoxyrhaponticin and serum albumin characterized by spectroscopy combined with molecular modelling

光谱法结合分子模拟表征脱氧土大黄苷与血清白蛋白的分子作用机制



全 文 :ža^Ÿå¯ ¡¢Ï£¤¥¦5§ôÎ
}¨Â©Âƒ¯ †‡Š‹
¶:
!
!
ؤÙ
!
ÚÛ

Q™RSG    m
!
Q™ áB
))$$#

gh
#!

#
Œ$,‚œ¯¤¥¦5§ô
!UTK.76SON[.1QP-P1$EB8
Ό}¨Â©Â
!OW/N1 KTSW/NVFW/P1$D83
ƒ
¯ †‡Š‹
(
ƒ„
#
¡¢Ÿ2”•p
$
*»Š¡¢()Ÿåhžža^Óñ[žaŒ,æΩ«ƒŸåŠ‹
(
…†
#
¯ ¡¢pG
EB8
Î
D83
ƒŸå¡ï
$
Ïi1{,æΩ«ƒÈ¡†‡Ý¨,x8-†‡Ý
$
{DÅʆ‡
(
žaÎð
Ïi
EB8
Î
D83
ƒÈ¡†‡Ï3S`LŸåST
$
Ÿå7ÊÔ7
$EB8
Î
D83
¯ ƒŸå/³
*
ÍÔ:
$
eicŸ?‹Û“
’3Ñ
( EB8
|
D83
ƒŸaüLÑ蟡¢
$
‰Ÿåoaƒ8-‚cŸhi
(
hžÈg()Ê°E
EB8
Î
D83
†_
ÑïLƒivS
*
;L
+
¡ï
( D83
Î
EB8
¡†ƒãݍ?Ïi
EB8
Î
D83
yÔxY8-†‡S¨ƒ¯ Ô†‡
(

OÆÀÛýi?
D83
Î
EB8
È¡†‡ST$Ÿœ?EçXäåæ
(
…‡
#
žaÎðÎ*»Š¡¢Ÿ/±3
$
SŒ
EB8
Î
D83
È¡†‡¤«Gc±À.
(

ijk
#!
¤¥¦5§ô
)
Œ}¨Â©Â
)
žaÎð
)
¯ ¡¢

lm)(
#!*$)5$%5#

nopP
#!
%d´;&Ɩfg
!*$(%%$%*

qers
#!
!
æi
$B5/NPV#GW./P1G2YNRWdTUWd-1
!!
}¨Â©Â™DÑB8@RcèæÓ[@,æ
¯ °~,Ÿ2Š‹
,-
(
}¨Â©ÂÈ|¯ «
ÛÔ:
$
ÄʂÔ5
%
}À‚Ô’
%
Îâö™DԒ
ƒgӂ
$
jܯ³
%
Gþ£‰N;Ÿ_¤
$
x2
ûƒ¡ï©Â«¯ 
(
Œ}¨Â©Â
!OW/N1 KTSW/
NVFW/P1$D83
,*-
S
(
AÚÆânƙœƒÿ4
„©Â«
$
‰ÚÆâmt½VD
)
AԑÚân
Æ
$

%
|;ÉÊÓ±A´ÿƒÔ‘ÚânÆ
$
;ÉÊ$D
(
|™œ‚q;ÉÊ
$
žD#6
;
Šƒ
xÿA;ÉÊ
( D83
VD
(
AëÚânÆ
$
Ç
*&
ooVD±A.ÚânÆ
$;
ŠSÏÙÚânÆ
$<
ŠSBÚânÆ
(
¯°Ÿ2”•p,æÎ
D83
ƒ
È¡†‡Y¨©,æƒ,ölP
$
?Ai,
æì$Š‹
$
>DñÜS,毰Gc2ú.
$
Z
x™D‚Lƒr†
(
¤¥¦5§ô
!UTK.76SON[.1QP-P1$EB8$
¯  
S
<
*
D
*&
(
$
xÏà5§ƒDöœ¯y±
$
™D—}
ã
%
—}}
%
0¸æ
%
„…Šöefef
%
0}rÓ0
¥(°†‡
,)-
(

$
¤¥¦5§ôΌ}¨Â©
ƒȡ†‡ŒeJHòaz
(
¤HÆܞa^
Ÿå¯ ¡¢()Œ?
EB8
Ÿå
D83
ƒÈ¡†
‡
$
¨©?Ÿå†_I?
%
ãÝ?°W.ø?,
æ|©Â«у¡¢
$
¯ -‚oþ
EB8
Î
D83
ƒÈ¡†‡Š‹
(
!
Ú£Î&^
\!
ibÎïù
!
Œ}¨Â©Â
!D83$
%
4(g%
¤¥¦5§ô
!EB8$
%
4(g%
NÝ2ÆÚÆ2C
!HSPK$ C>
K~ÜoJ]ǟærTFG
)
‰Òï
ùKS¯°þïù
$
Îð‡-SÓZlUʳ -
(
⋎ÊS
$d /.V.@
e
$[D%d&
ƒ
HSPK5D‡ù
Ä/
!
8V
$d$ /.V.@
e
;N1{³ 7Ê
$
‡
Z‡ùÄ/â‹
d$ l$
e
/.V.@
e
ƒ
D83$
Äî
Ä/â‹
d$ l$
e)
/.V.@
e
EB8
Ä/
(
?5&$$
ïhžžÊ*
!

DPQN-OP
FG
)]J5
*&$
ïñ[
5
J¯žžÊ*
!

8OP/NUYW
F
G
) E`5*
ïtŠâÊ*
!
oJÎIibí
)8C:\*
*»Šg|r†É
$E\<^
¼•
!&d$*
ä¤
(
\(!
¯ ¡¢
!
ɇ
8C:\*
r†Éoƒ
E\<^
&d$*
TtæpG,æΩ«ȡ†‡¡ï
$
Žd
¯ ¡¢*»
( D83
ªöŸƒ
AEI
Rm
#O4Y(
r™p
EB8
¯ Ÿ
$
;ÛÆܯ 
Ý
99*
Ýì(۟œÎ剆ï
$
¯ |#
å%2Q‰‡
3WQ.E.-Z H..VZPQ!3EH
Žd¨öÎ
.%$.
t
)4
ct
#
v
*$&
o
)
p
!!!!!! ! ! ! ! ! !!!! ! ! ! !
J.Vd)4$:KKWT!#
9NS-O$ *$&
âöƒ(%2
(
¯ |#()-]
D83
‚1
o
$
¶‰âöS$Uƒ
${
ûØS|#ƒ‚$
U
(
-À‚1o=f
#
#-À
EB8
Ÿå‚o
[
)
$ÇâöŸåo[*øÏíYZ´¨öŸåÖ
«
)
%‰ï¯â‘:Ï$Uƒ/³Óâö
$‘¾ ƒ/³
$
ŽdâöǨö‚o[%ƒ
Ñ(Ø
)
6Yðº‹?†SgX?
$
‹â
öΨöƒ«NŸå& 
,&-
(
Ttƒ2¯?¶
‰TtÌ]ƒìAÓb?
(
T|pGƒ
D835
EB8
¯ |#öeŽd
ST5U.-Z
ðý
$
ÊI
EB8
Û
~µ|#Ÿœµƒ
D835>L?!>5+NSRP1T
¯ |#
öeWÓ|
AEI
0?¯$¾|#öeY-ý
 ‚
(
\&!
&^
!D83
Î
EB8
Ä/ƒhžžaˆ
EB8
Ä/ƒÑҞa;À
#
’H
*d /@D83
Ä/Ü

-/
ßàÓ.¼$
$
üێ®b̦¢A
d$ l$
e)
/.V.@
e
EB8
Ä/ŽdhžƒÀ
!
ƒÀùÒ¢ö
d$
$$

@$
‡ùÄ/‘hžÉÂÔ^
$
hžc´
μcnÈ`ÊKS
*d 1/$
! îX
*$$ 1/.
/P1
e
$
úÀ¼c! 
*(* 1/$
°\pr‹
*$ o4$$
1/
ƒc´a
)
hžžaiîï¼c! Óc´! 
yԃ! s¯­S(&
1/
Ó(&
#$ 1/
oUÄ
/öeƒ‘=hžža
(
c´Î¼cnÈ`ʑ
o
$
îX
*&$ 1/./P1
e
$HSPK5D‡ùÄ/‘hž
ÉÂÔ^
(
’H
*d /@HSPK5D‡ùÄ/Ü
-/
ßàÓ.¼$
$
üێ®b¢A
d$ l$
e)
/.V.
@
e
EB8
Ä/
$
‰‰ŽÊS
d$ l$
e
/.V.@
e
$
;
À
$$ o4$ 1/
ñ[ÑҞa
$
$Xîï
$
nÈ`Ê
*d 1/$
‡ùÄ/ÀƗ
(
\*!
2úƀ
!
©Â«
5
,æÈ¡†‡ƒhž{Š‹
#
©Â«
5
,æÈ¡†‡ƒ`Lhž{\STï
õ
8QTS15J.V/TS
&T
,-
(
K
$
=Kn rG
;
)
$
,%- n rG
>X
,%- !
 $
#G
;
S…¯ {\STXžI?
$
)
$
S{ù1ÇQŸæ5¯ ƒ‚Kðì
$,%-
S{\ù
!EB8
ƒŽÊ
$G
Kc
S
8QTS15J.V/TS
{\I?
(
©Â«
5
,æÈ¡†‡ƒ2ú&T
#

5
©Â
«È¡†‡*‡&T
!*
¨©È_ƒŸå?
,#-
(
VG,!K
$
eKQK- nVGGr1VG,%
8
- !*
K
$
Î
K
¯­S{\ù¢A¬Ûhž¯ ƒÈ
|7Ê
$G
xŸåI?
$1
xŸåo[?
$,%
8
-
S{
\ùƒ…³ŽÊ
(
?|SKQTS
E;´‹Û“’2ú
#?|SKQTS
E;´‹
ۓ’2ú
,%-
’»,æ
5
©Â«‹Û“’öž
M$
Ÿ
å/³
*
ˆí³‹Û“’/³
C
$
&Tñp
(
MnC
$
#
=!C
$
#
r*
#
!)
M
S‹Û“’öž
)C
$
S‹Û“’öž
M
S
$g
Qƒí³‹Û/³
(
C
$
#
n(d( l$
e*
G
*
B
e&
*
T !&
 $
#G
*
SÐÏÉÔHõË 
)B
S9«ƒ¤´
i?
)
*S©Â«¯ ƒžÛ öž
)T
S©Â«¯
 hžc´žaÎ,æÑҞaԃža~¥

(
Tn

f
$
K!
&

+
!
&

&
&
<
&

f
$
K!
&
<
&
!
 $
#K!
&

S©Â«¯ Ç! &%ƒhž7
Ê
)
+
!
&

S,æÇ! &%ƒ&kÑÒe?
(
‹Û“’öž
M
“YP¯p ;©
(
Mn eK=K
$
!#
hžOÆÊ
2
Înõt‚
*

#
OÆhžž
a‡OÆÊ
2
Înõt‚
*
VÊÛ
,(-
$
*»F 
ñp
(
2Z!N
XX
[FN
XL
Q!N
XX
\FN
XL
!%
‰$
F
SÔ^Ë 
! FZN
LX
QN
LL
$N
XX
Ó
N
XL
¯
­Sñ6Oƞ¼cpƒñ6Oƃc´ž7ÊÓ
-‚Oƃc´ž7Ê
$N
LX
Ó
N
LL
¯­S-‚OÆ
ž¼cpƒñ6OÆc´ž7ÊÓ-‚OÆc´ž

(
*Z
N
XX
[N
XL
]F
N
XX
\*N
XL
]F
!(
(!
Ÿ/ÎYú
(\!
¯ ¡¢Ÿ/
!
¯ |#¡¢*»Q
$

D83
ƒ
8WUV.+pKKPQTK:$KPQTK:
,4-
$#
AãxâŸåo

+NSRNSP1
Ÿåo[Žd
EB8
¯ |#ƒ¡¢*
»
$
¯ |#BS?ƒ|ÓVò,毠ƒ«
Ÿåo[Ρ 
(
¯ |#
EB8
Î
D83
ƒŸå
¡ïJg
!
ëpâöŠØ
$d${
ûØ8ƒÚÆâ

( EB8
¯ ŸåÇ
D83
ƒÏ(‚+¹%
$
„A¯ %Ü6æØ4L
( EB8
¯ /
D83

ânÆ
!HS[*&
ˆHêÚânÆ
!AOT*$#$AOT*$
AOT))$
Å6
$
àŒÊþ?
EB8
‹D{D83
8
@hžƒ3Ñ
(
‘Q
$EB8
¯ Î
D83
ŸåST
$
$EB8
¯ $HOoÝƃ¥¾ Î
@TW&(

.#%$.
t
)4
ct
#
v
*$&
o
)
p
!!!!!! ! ! ! ! ! !!!! ! ! ! !
J.Vd)4$:KKWT!#
9NS-O$ *$&
ÅÊÈ¡†‡
$
£
EB8
O¥Oo
*)
*
Ýƃ¥¾ ¯­Î
@6K44$8TS*$*
cŸÅÊÈ¡
†‡
)
$±&(
$EB8
EçXŸåÇ
D83
‚o[
a
$
‰Ÿå+¹ŠØ¨,ÿ
@TW4($ @TW*$)$
@TW)*%$@TW))$@TW)&%$@TW)&4$@TW&($3VN*$$
3VN*$$3VN*)$ 3VN*$ AOT*$#$ AOT*$ AOT))$$
HS[*&$JNV)&)$JNV)&&$JNV&(*
ÚÆânÆ|œ±
A8-La
$
Y¨#÷ø‹
EB8
Ÿåo[%ƒ8
-È¡†‡
$
É8-†‡Ç
EB85D83
äåæ|œ$
½~,†‡
$
8-†‡x,æө«¯ Ÿåƒ
¨,ó`Ý
(
¯ ¡¢Ÿ/Žd
ST5U.-Z
ðý
$
c3
3WQ.E.-Z
|Zöe 
(
g
!EB8
Î
D83
È¡†‡ƒ¯ |#g
!3
ˆ8-Ï(
g
!I
?PGd!:1QTSN-QP.1 /.UT!3 N1U O6US.[O.FP-KWSRN-T/N[
!I FTQ+TT1 EB8 N1U D83
ÿ¯ ¡¢Ÿ/¤
EB8
Î
D83
ƒ¨,†‡
ÝS8-†‡Ý
$
{DÅʃ1Ç
$
Sðý
EB8
Î
D83
ƒÊ劋ÓÊå& 
$
BSžaÎðŽ±=
ýί ¡¢ƒå2‚
(
(\(!D83
Î
EB8
È¡†‡ƒhžžaˆŸå†_
Š‹
!D38$EB8
ˆ;êY°æ«ƒÛŽåöeƒ
hžžaJg
*(
Ç
*(* 1/
¼c! p
$EB8
ƒ«
5c´! S
)4) 1/
™˜
$D83
«5c´! S
))4 1/$
ÿ
EB85D83
öeƒhžžaYÃE
$
,惢A‰
D83
ƒhž7ʗ˜
$
Ïi,æÎ
D83
yÔ1ÇüÈ¡†‡
$
cŸ?‹Û“’
(
3d* q)Id)% q)dD83!,
D83
n l$
e
/.V.@
e
) *dD835EB8
!,
D83
k,
EB8
nk))dEB8!,
EB8
n l$
e
/.V.@
e
(
g
*!D835EB8
öe$nﮃhžga
?PGd*!?VW.STK-T1-TT/PKKP.1 K[T-QSN.RD835EB8 K.VWQP.1 K6K5
QT/
EB85D83
ƒñ[ÑÒsaJg
)$
Yc3
D83
ƒñ[ÑÒߗˆ
EB85D83
°&kŽåæÎ
EB8
ƒsaªÚqm~å
$
Ïi
EB8
ƒ¢AWe‰
D83
ƒñ[ÑÒcŸi(
$
Y~=-À
EB8
Î
D83
¯ ƒhž{\Š‹S`L{(
‘Qÿg
)
J
$EB8
Ç
*(* 1/
¼c! ˆ
))4 1/
c´! 
ppDÑÒ
$
ËZ
$
.Ð8Ǟö_
(
¯°g
&
¤
$N
ÌS
D83
ƒ±;hžÌ
$
Y
ÃEëü,惢A
$N
ÌÌ͗˜
)F
ÌS
EB8
ƒ
±;hžc´Ì
)-
ÌS¼c̃;;§hžÌ
)
U
ÌS
D83
ƒ;;§hžÌ
(
úÀ
D83
ŽÊ
$
ë
ü,æŽÊ¡5
$D83
ƒhž7Ê¿õ®‚ƒ—
˜
$
àÏi,æ|}¨Â©ÂD{\†‡
(

.%%$.
t
)4
ct
#
v
*$&
o
)
p
!!!!!! ! ! ! ! ! !!!! ! ! ! !
J.Vd)4$:KKWT!#
9NS-O$ *$&
!!!
dD83!,
D83
n l$
e
/.V.@
e
) *dD835EB8!,
D83
n,
EB8
n l
$
e
/.V.@
e
(
g
)!EB85D83
ƒñ[ÑÒsa
?PGd)!3FK.S[QP.1 UPRTST1-TK[T-QSW/.REB85D83
«5c´ÌoE3
*$ 1/
ƒO’
!3$I
¡gKñ
Z
$
ei
EB8
Ó
D83
-Î1ÇÈ¡†‡
$
îœ
D83
¯ $Î
EB8
È¡†‡ƒÚÆânƃüL
O|cŸ±Ài(
(
3d* q) I#)% q),
!EB8
=$
e
/.V.@
e
$ o(
¯­S
$d$$ $d&$
$d($ d$$ d#$ *d&$ )d*$ &d$(
g
&!D83
Î
EB8
È¡†‡ƒhžga
?PGd&!?VW.STK-T1-TaWT1-OP1GK[T-QSN.RD83P1 P1QTSN-QP.1
+PQO EB8
S-À,æ|©Â«hžƒ{\Š‹
$
ÿÎð

EB8
Î
D83
È¡†‡ƒhžža?¯
$
&
T
!
%2©g
(
g
!EB8
|
D83
hž{\ƒ
8QTS15J.V/TS
g
?PGd!8QTS15J.V/TS-WScTK.RD83RVW.STK-T1-TaWT1-OP1GF6
EB8
ÿÜ){\ùQŸæ5¯ ƒ‚Khžðì
7
$
n l$
e(
K
,$-
$
“ÿÎð?¯YBS
8QTS15
J.V/TS
&T*»‘\Êp
EB85D83
È¡†‡ƒ
{\I?
G
6
$G
;
$
*»Ÿ/JÏ
(
Ï
!D83
Î
EB8
È¡†‡ƒ{\?
HNFVT!HOTaWT1-OP1G-.1KQN1QK.REB8 Q.D83K.VWQP.1 K6KQT/
$=^
G
Kc
=l$

G
a
=l$
)
C
*4( $d($( $ (d$($ $ $d44)
)$ d$*) $ &d) $ $d44# )
g¬Ÿæ5¯ Î‚:¯ È¡†‡Œ
$
$

*
ª&^\Àhž{\Š‹
$
±ª&^xÓ
ԅ¯ {\STXžI?Î{\ù|Ÿæ5¯ 
«5^ûô¥{\XžI?VÔ#\]
,-
)
$±ª
&^xBSi\Îð6#Ä]
,*-
(
¤ÎðS-À
EB8
|
D83
ƒ{\Š‹
$
ǐ‘\Êp†
EB8
|
D83
hž{\ƒ
8QTS15J.V/TS
gJg
(
Âg


J
$
ǶÀƒŽÊûØ8
$
ëü\Ê0Ž
$
{\I?
¿¡5›º
$
~=Ïi
EB8
|
D83
ƒ{\Š‹S
`L{(
$[
$
\]ÙL{\Ó`L{\ƒ$±
A&^x.øhžæ«ƒÑҞa
(
ÙL{\$
$
ÆLâåæƒ|œ+F½hžæ«ƒÑҞa]
i(
$
`L{\ÐС¢hžæ«ƒ¼cL
$
¡
¢hžæ«ƒÑҞa
$
¯Z\]Göex³±
Ü`L{,)-
(

EB85D83
öeƒÑҞa
!
g
)$
c3
D83
ƒñ[ÑÒaÓ
EB85D83
Žå
æƒñ[ÑÒaÎ
EB8
ñ[ÑÒsaªÚqm~
å
$
Ïi,惢AWe‰
D83
ƒñ[ÑÒcŸi
(
$
ÿZ
*
AŸ/Y-À
EB8
Î
D83
ƒhž{.(%$.
t
)4
ct
#
v
*$&
o
)
p
!!!!!! ! ! ! ! ! !!!! ! ! ! !
J.Vd)4$:KKWT!#
9NS-O$ *$&
Š‹S`L{(
(\&!EB8
Î
D83
È¡†‡ƒŸåI?
!G
ˆŸå
o[?
!1!
¤Îð*‡&T 
!*
¢åÎð?¯
$
Y
VG,%-
|
VG,!K
$
eK=K-
†…|?gJg
#$
’
©
G
Ó
1$
Ÿ/JÏ
*(
,
!D83
nd$ l$
e
/.V.@
e
$ ,
!EB8
nd$ l$
e
/.V.@
e
(
g
#!EB85D83
öehži(—‚¢åg
?PGd#!?VW.STK-T1-T-ON1GTVP1TNSRPQP1GSTKWVQK.REB85D83
K6KQT/
Ï
*!D83
Î
EB8
ƒŸå?
HNFVT*!HOTFP1UP1G[NSN/TQTSK.REB8 Q.D83
öe G=@./.V
e
1
¢å&T
C
D835EB8!*4( ^
)d#$% & l$

$d4& ?nd% * r$d4)4 @ $d44
D835EB8!)$ ^
d*(( ( l$
#
d$* ?n#d$ * rd$* 4@ $d44( (
!!
g
#
ŸåÏ
*
J
$EB8
Î
D83
yÔ1ǟ
åÔ7ƒÈ¡†‡
$EB8
Î
D83
1DZAŸåo
[
$
ei
EB8
¯ Î
D83
|œ?Ô}Àƒä
åæ
(
(\*!EB8
Î
D83
È¡†‡Ý¯°
!
,æΟ
æ5¯ ƒÈ¡†‡ÝæxÅÊ
%
û@]Ý
%
Ù
WFÝ
%
8-È¡†‡
$
DZÀ\ÊûØ8
$
È¡
†‡STƒ 8
L
ôSI?
$
“P¯
!4 $!$ $
!
ãÝF 
,&-
ˆ 
!*
’EƒŸåI?
G$
Y*»
EB8
Î
D83
Ÿå†_ƒãÝ?
$
Ÿ/JÏ
)(
(
Fn
(
Le$.
(
> !4
(
FneC$:1G !$
V1!G
*
QG

n!
(
LQC.!Q$

eQ$
*
!
,æΩ«ȡ†‡ƒCïÿñpõ“-
À
,-
#
(
>s$
QS8-ÓÙW†‡Ý
)
(
> h$
QS
ÅÊÓû@]Ý
)
(
Ls$$
(
> s$
QSûïƒ8-†
‡Ý
)
(
Lh$$
(
>h$
QSÅÊÓû@]Ý
(
Ï
)!EB8
Î
D83
ƒãݍ?
HNFVT)!HOTQOTS/.U61N/P-[NSN/TQTSK.REB8 FP1UP1G+PQO
D83
öe
(
L
=ZX./.V
e
(
>
=X./.V
e
.^
e
(
F
=ZX./.V
e
D835EB8 !*4( ^ (d$ )%4d(% e)d%$
D835EB8 !)$ ^ (d$ )%4d(% e)#d*#
ÿÏ
)
Ÿ/¤
$EB8
Î
D83
ƒÈ¡†‡¨,
S8-†‡Ý
(
(\$!EB8
|
D83
öeƒ‹Û“’?;À
!]J
aˆhža†~¥žagJg
%(
Âg
%
J
$D83
ƒhžc´aÎ
EB8
ƒñ[ÑÒa-D1¯~¥
(

?}SKQTS
ƒÐÏ
5
ÐÏE;´‹Û“’2ú
,#-
i
E
#
`c‹öΨ‹ö‘D 6
$
/³Ç
% 1/
ûØ
8Q
$
6¼cƒc‹ö¯ a+Î6ͨ‹ö¯ 
cŸE;´‹Û“’3Ñ
(
ñ/c‹öc´hž
$
c‹öƒhžc´žaaΨ‹öƒÑÒaD‘D
ƒ~¥
$
3c‹öhž{$
“Y’E¨‹ö¯
 Óc‹ö¯ ƒŸå/³
(
¤ÎðP¯F 
!
*»E
D835EB8
Ÿå†_ƒža~¥d¯
Tn
d( l$
e&
-/
)
.@
e
./.V
e
$
ÿF 
!#
’©‹Û
“’öž
Mn$d&#(
ǤÎð”•p
$
HÐÏÉÔ
HõË 
G
*
n*=)$
žÛ öž *
n$d($
¤´i
?
Bnd))#$
aYonÛRAF 
!&$
©‹
D83
ƒí³‹Û/³
C
$
n*d%* 1/$
¶RAF 
!)
’©Ÿå/³
*
S
*d%4 1/( *h% 1/$
É
D83
ƒÚ
ÆânÆÎ
EB8
ƒ/³:Ü
% 1/$
 åE;´‹Û
“’2ú
$
ÚÆânƁa‹ÛYE;´& “’
‡
EB8$

D83
ƒhž{(
g
%!D83
ƒhžc´a
!N
Î
EB8
ƒñ[ÑÒa
!F
ƒ~
¥g
?PGd%!\cTSVN[ FTQ+TT1 QOTRVW.STK-T1-TT/PKKP.1 K[T-QSN.R
D83!N N1U ]JNFK.S[QP.1 K[T-QSN.REB8 !F
.4%$.
t
)4
ct
#
v
*$&
o
)
p
!!!!!! ! ! ! ! ! !!!! ! ! ! !
J.Vd)4$:KKWT!#
9NS-O$ *$&
(\!EB8
|
D83
у¡¢
!
©Â«‘=hžž
a$
$
(&
n 1/
ˆ(&
n#$ 1/
¯­ëpëÚân
Æ
%
.Úânƃhžža˂
(
©Â«$ÚÆâ
nƃ«5c´! Î‰*%O|ƒÏ‚D+
$
œ
ÿ«5c´! ƒi(\]©Â«уi(
(
úÀ
D83
ŽÊ
$
ÌÍ¡¢
EB8
ŽÊ
$
©‹
D835
EB8
öeƒ‘=hžža
$
Jg
((
Âg
(
J
$
D83
ƒhž¨,ÿ.Úâ*‡ò
(
ëü,æŽÊ
ƒ¡5
$
ëÚânƃ«5c´! Æ¤g{i
!
g
(
$
3$
g
(
$
I
Ç
*4$ 1/
%|œ?±AÌ
$
àAÌWE
D83
©Â«Ì
$
x
EB8
Ì
(
(&
n#$
1/
Q
$
.Úânƃ«5c´! E3?áüò’

$
Ïi.ÚânÆ*%O|ƒ8-‚D*¡¢
$
™œ8-փ4„%Ü1¯›¯4L
$
ŽF½
D83
ÑcŸ?±ÀTʃi(
(
3d
(&
n 1/) Id
(&
n#$ 1/(
g
(!D835EB8
öeƒ‘=hžža
?PGd(!BRT-Q.RQOTEB8 .1 QOTK61-OS.1.WKRVW.STK-T1-TK[T-5
QSW/.RD83
(\%!D835EB8
Ÿå†_|
D83
ÑïLivƒ
¡¢
!
*‡hžÈg^¯°
EB8
Î
D83
Ÿå†_
|
D83
ÑïLivƒ`݊‹
$
Çc´! 
&

Ó &
*
p;À©Â«Ç‘Îð”•phž7Ê
N!
&


Ó
N!
&
*

ƒi(VïU©Â«ƒŸi(
Ÿ/
(
N!
&

n-r/N!
&
*
!*
-nN

!
&

N

!
&
*

,N
*
!
&

eN

!
&

-
,N
*
!
&
*
eN

!
&
*
-
/n
,N
*
!
&

eN

!
&

-
,N
*
!
&
*
eN

!
&
*
-
¥+e 
!*
Ï3S—‚
$
“ÏpÈ_ƒ©Â
«ÑïLivST å
*
;L¡ï
+)
†y¥SE
—‚
$
Ïi©Â«ƒÑïL“iSTx±Ati
ST
)
¥+e 
!*
Ï3S
*
”R­”6—
$
‰$
u±”6—JïU±A
*
mR)
+
ƒ©Â«Ÿi
(ST
$
“ å
*
­L¡ï
+(
P¯Îð?¯†EƒÈ_hžÈgJg
4(
Â
g
4
YÃE
$EB8
Î
D83
Ÿå†_ƒhžÈg¿
—‚
$
ei
EB8
Î
D83
cŸEçXŸå†‡Q
$
D83
$ÑïLivSTx±tiST
$
x±A
*
;L
+
¡ï
(
3d* q) Id)% q(
g
4!EB85D83
öeƒhžÈg
?PGd4!HOTRVW.STK-T1-T[ONKTUPNGSN/K.REB85D83K6KQT/
(\`!EB8
|
D83
.ÚâüLƒ¡¢
!
úÀ
D83
ŽÊ
$
¢A‘ŽÊƒ
EB8
Û
$
HOÆhž?
¯WF 
!% $!(
*»nÄ/öeƒ
2
ˆ
*$

&(
hžöƒOÆÊ
2
ˆnõt‚
*
Îhžöƒ“
`Xʜ†Ó
(
hžöØ:
$
“`XÊØW
$
‰OÆ
.$($.
t
)4
ct
#
v
*$&
o
)
p
!!!!!! ! ! ! ! ! !!!! ! ! ! !
J.Vd)4$:KKWT!#
9NS-O$ *$&
!!
Ï
&!EB85D83
öehžOƍ?
HNFVT&!HOTRVW.STK-T1-T[.VNSPYNQP.1 [NSN/TQTSK.REB85D83K6KQT/K
öe
N
XX
N
XL
N
LX
N
LL
F 2 *
D83 %#d)$$ $ *4d($$ $ $&d$$ $ 4d&)$ $ d$4$ 4 $d$4 * $d$%
EB85D83 4&d4$ $ #(d%4$ $ #d&$ $ )d4$ $ d$ $ $d) % $d$4 (
Ê
2
ˆnõt‚
*
­Ø:
(
ÿÏ
&
?¯J
$
Ç-
Ä/$hžö¯ 
D83
“`XÊÔW
$
HO3ÑÅ
~
$
OÆʈnõt‚Ô:
(

D83
Î
EB8

È¡†‡Û
$D83
ƒOÆÊ
2
ˆnõt‚
*
KDG
Ž
$
ei,æΩ«¯ yÔ1ǯ Ô†‡Ý
$
|œäåæÛhžöödi5
$
¯ “`¨„
$

XÊid
$
öeƒˆÊi5
$
ˏhžOÆÊëyi
5
(
Îð?¯ÀÛýi?
D83
Î
EB8
cŸÈ¡†
‡
$
Ÿœ?EçXäåæ
(
&!
Ÿú
¤Hɇža^Ÿå¯ ¡¢Œ?
EB8
Î
D83
ƒÈ¡†‡
(
¯ ¡¢õp?,æÇ
D83
o
ƒŸå1o
$
TåÎ𨩃ãÝ?
$
-À?¡
êԃ†‡Ý
$
¨,x8-†‡{DÅʆ‡
(
ž
a^ƒŸ/ëp,毠Î}¨Â©Âyԃ†_
Š‹S`L{(
P¯‹Û“’2ú
$
ei
EB8
Î
D83
cŸ?E;´‹Û“’3Ñ
( EB8
ĢAF
½
D83
ÑïLƒiv å
*
;L¡ï
+(
P¯h
žOƯ°ýi?
D83
Î,æ-ÎcŸ?È¡†
‡
$
Ÿœ?EçXäåæ
(
¤r†S$,‚œ
¯¤¥¦5§ôΌ}¨Â©Â¯ È¡†‡ƒ?
AŒGcDpŸ/
(

vwxy
#
, -!
>M
$
Sià
$
ßOö
$
°
d
àRሉ¡ª‘¯töξ
}¨Â©ÂÈ¡†‡ƒã݌
,X-d
(a
$*$$%$#
!##d
, * -!DTb9$ KTSW/NVFW/P1,X-d;NQWST$ 44)$ )#&!#&)#*$4d
, ) -!\SON1 EE$ 3KVN1 9$ 8T1U.GUW ;$ TQNVdBcNVWNQP.1 .RQOTO65
[.GV6-T/P-TRT-QN1U N1QP.7PUN1QN-QPcPQ6.RQOSTTJPK-W/NVFW/
KWFK[T-PTK!BWS.[TN1 /PKQVTQ.T P1 KQST[Q.Y.Q.-P15UPNFTQP-SNQK
,X-dXBQO1.[ONS/N-.V$ *$$$ 4(!=*#4d
, & -!

d
Æܯ |#()ƒ:¯ 
5
©Â«È¡†‡Œ
,E-d

#
D95
$ *$$4d
, -!@NZ.+P-YX>dASP1-P[VTK.RRVW.STK-T1-TK[T-QS.K-.[6,9-d
HOPSU BUPQP.1dITP0P1G#8-PT1-TASTKK$ *$$(d
, # -!
øË
$
-ð<
$
!ӆ
$
°
d
hž^Œ¼³ËÓ·Dξ}
¨Â©ÂÈ¡†‡
,X-d
žaΞa¯°
$ *$$#$ *#!)#
$d
, % -! O`N1GCL$ _WT_9$ AN1 XD$ TQNVd8QWU6.RQOTP1QTSN-QP.1
FTQ+TT1 P-NSP1 N1U OW/N1 KTSW/NVFW/P1 F6RVW.STK-T1-TK[T-5
QS.K-.[6,X-dX9.V8QSW$ *$$($ ((!=)#)*d
, ( -!@NZ.+P-YX>dASP1-P[VTK.RRVW.STK-T1-TK[T-QS.K-.[6,9-d
HOPSU BUPQP.1d;T+M.SZ#AVT/W/ASTKK$ *$$#d
, 4 -!ATQTSKHd3VNF.WQNVFW/P1#FP.-OT/PKQS6$ GT1TQP-KN1U /TUP-NV
N[[VP-NQP.1K,9-d8N1 EPTG.#3-NUT/P-ASTKK$ 44#d
,$-!XPN1G<_$ CN.9b$ DTXbd8QWU6.RQOTP1QTSN-QP.1 FTQ+TT1
QTSNY.KP1 N1U KTSW/NVFW/P1 K61-OS.1.WKRVW.STK-T1-TUTQTS/P1N5
QP.1 .RQTSNY.KP1,X-d31NV,-!DTLM$ @PM$ 8PD $` TQNVd9.VT-WVNS/.UTVP1GN1U K[T-QS.5
K-.[P-KQWUPTK.1 QOTFP1UP1G.RGWNPN-.VQ.OW/N1 KTSW/NVFW/P1
,X-dXAO.Q.-OT/AO.Q.FP.V3$ *$$#$ (*!*#(d
,*-!
!7
$
>Éà
$
nO<
$
°
d
§ùC,æΌ}¨Â©ÂÈ¡
†‡ƒŒ
,X-d
Ž°Ô(a
$ *$$ )*!*#*&d
,)-!DTLM$ DW `E$ MN.bX$ TQNVd<./[NSPK.1 .RQOTP1QTSN-QP.1
.RNV[P1TQP1 N1U -NSUN/.1P1 +PQO OW/N1 GN//NGV.FWVP1,X-d3-5
QN,&-!
ýúX
$
oûü
$
ý–7
$
°
d
…þÐÖξ}¨Â©ÂŸ
åãÝË£ƒhžža^Œ
,X-d
žaΞa¯°
$
*$$)$ *)!)#&)d
,-!>.KKAE$ 8WFSN/N1PN1 8dHOTS/.U61N/P-K.R[S.QTP1 NKK.-PN5
QP.1 STN-QP.1K#R.S-TK-.1QSPFWQP1GQ.KQNFPVPQ6,X-dIP.-OT/PKQS6$
4($ *$!#)$4#d
,#-!@P1 D$ @N1 X?$ CWN1 9$ TQNVd8[T-QS.K-.[P-P1cTKQPGNQP.1 .R
P1QTSN-QP.1 FTQ+TT1 /N1GPRTSP1 N1U F.cP1TKTSW/NVFW/P1,X-d
8[T-QS.-OP/3-QNANSQ3$ *$$4$ %)!#4)#d
.($.
t
)4
ct
#
v
*$&
o
)
p
!!!!!! ! ! ! ! ! !!!! ! ! ! !
J.Vd)4$:KKWT!#
9NS-O$ *$&
=A0@KH0.F.KC/AB-@KD.B/J-ALG@JAPEFD.MABC/K/B.BGJ@FH-.0NH-/B
KD.F.KC@F/Z@GNE JM@KCFAJKAME KA-N/B@GU/CD-A0@KH0.F-AG@0/BT
C]\9P1G
!
$ ?3;LT157PN1G$ D]>W15OWNP
!>,4++=+8>,&)1,)$ H4)I&-1D !D*&,.=(.*)JK+*)(*?E1&6)*&(?$ :&1Y-1 ))$$$ #4&1-
,5NJCF.KC-!7NW@KC/R@# H.KQWU6QOT/.VT-WVNSN-QP.1 /T-ON1PK/.RN-QPcT-.1KQPQWT1QKUTK.76SON[.1QP-P1 !EB8 N1U OW/N1
KTSW/NVFW/P1 !D83d=@CDAG# ]1UTSQOTKP/WVNQTU [O6KP.V.GP-NV-.1UPQP.1$ -./[WQTSN1NV.GQT-O1.V.G6$ RVW.STK-T1QK[T-QS./TQS6
N1U WVQSNcP.VTQK[T-QSW/+TST-./FP1TU Q.KQWU6QOTFP1UP1G/T-ON1PK/FTQ+TT1 USWGN1U [S.QTP1d9@JH0C# 9.VT-WVNS/.UTVP1G+NK
NU.[QTU Q.TKQNFVPKO QOTFP1UP1G/.UTVFTQ+TT1 EB8 N1U D83$ KWGGTKQP1GQONQQOTP1QTSN-QP.1 R.S-T/NP1QNP1P1GUSWGN1U [S.QTP1 PK
/NP1V6QOTO6US.[O.FP-P1QTSN-QP.1 +PQO NO6US.GT15F.1U P1QTSN-QP.1dHOTSTKWVQKRS./K[T-QS.K-.[6P1UP-NQTU QONQQOTP1QTSN-QP.1 FT5
Q+TT1 EB8 N1U D83PKNU61N/P-FP1UP1G[S.-TKK+PQO NOPGO P1QT1KPQ6dHOTcNVWT.RQOTFP1UP1GUPKQN1-T!* FTQ+TT1 EB8 N1U D83
+NKV.+$ +OP-O UT/.1KQSNQTQOT.--WST1-T.RT1TSG6QSN1KRTSdEB8 /NUTN1 P/[N-Q.1 D83pKQSW-QWSNVU./NP1 /P-S.-TV-.1R.S/NQP.1$
+OP-O STKWVQTU P1 O6US.[O.FP--ON1GTKP1 FP1UP1GNSTNKd3--.SUP1GQ.QOTRVW.STK-T1Q[ONKTUPNGSN/QT-O1P-NVN1NV6KPK$ QOT-ON1GTKP1
QOTEB85D83STN-QP.1 -.1R.S/NQP.1NV[NQTS1 KO.+TU NjQ+.5KQNQTj/.UTVd3--.SUP1GQ.QOT.FQNP1TU QOTS/.U61N/P-[NSN/TQTSKR.SQOT
EB85D83P1QTSN-QP.1$ QOTP1QTSN-QP.1NVR.S-TFTQ+TT1 EB8 N1U D83+NK/NP1V6NO6US.[O.FP-P1QTSN-QP.1dHOTRVW.STK-T1-T[.VNSPYN5
QP.1 [S.cTU QONQN1.15-.cNVT1Q-./[.W1U +NKGT1TSNQTU UWSP1GQOTP1QTSN-QP.1 FTQ+TT1 EB8 N1U D83d1ABK0HJ/AB# HOTK[T-QSW/T75
[TSP/T1QKO.+TU -.1KPKQT1QSTKWVQK+PQO QOT-./[WQTSN1NV.GQT-O1.V.G6$ +OP-O -.WVU [S.cPUTU -TSQNP1 STRTST1-TR.SKQWUPTK.1 QOTP1QTS5
N-QP.1 FTQ+TT1 EB8 N1U D83d
, @^E UAFGJ-!UTK.76SON[.1QP-P1) OW/N1 KTSW/NVFW/P1) K[T-QSW/T7[TSP/T1Q) /.VT-WVNS/.UTVP1G
GA/#)_*(`QKWK--()*)(*

z{-0
!
‘‘
#
.*($.
t
)4
ct
#
v
*$&
o
)
p
!!!!!! ! ! ! ! ! !!!! ! ! ! !
J.Vd)4$:KKWT!#
9NS-O$ *$&