免费文献传递   相关文献

Screen anti-influenza virus serum marker of Lonicera japonica by proteomics technology

应用蛋白质组学技术筛选金银花抗流感病毒血清标志物



全 文 :\“yv[Ù_ùÓÖ¶t—
¯×dÏØSóÀ¦Ì
EÛl
R
#

X
#
Ôܚ
X
!
!R:
&à#5EHIJG
#
&à ;1
XLMMR_% X:
&à5EH>¬
#
&à ;1
XLMQLL
$
‚;
%!

&
Š3YZ¦§Ù[8‰Sóyv[EGT)t—¯×dÏØ`¶SóÀ¦Ì
!
êŠ.t—¯×d
ÏØE“[6ÃGìܵq¥)

„…
&
“z†/{
$X6¡YZ¦§Ù[8‰Sóyv>Š3EG
!
¶$Sór.wE
G¼myv-
!
#Ë[ŒÕÔÑãÌnü_6

†‡
&
Í
X)KRL

!
>m 
=>CT;#Ë<01ÕÔ
!
0nŪr[
RX
àiyv[Ú6:E

†›
&
YZ¦§Ù[8‰Sóyv
g’Ž].wEG
!
m yv[Ù_…©Ö¶>ÕÔK@ÌSóÀ¦Ì
!
êŠ.t—¯×dÏØE“6ÃGìܵq
¥)

$
ˆ‰Š
%!
t—
&
yv[Ù_
&
×dÏØ
&
À¦Ì
$
8‹$f
%!XMRMMYXYMML
$
ŒEq
%!
¬üRnëø_stuv
$dXMM\R\\%
$
Ž€>
%!
!
ŸŽ
!
¾¿
!
ÚCãçÀ
!
‚&³ÙrÇ[·GÜ
ƒ„NE
!A(&,$MLQR%NXYRQ_YQ!-$
>‘
%!
¢æ
!
BC
!
ƒ„¦½,
!
‚&³ÙrÇ[·irÇ
^_…Vƒ„
!!
yv[Ù_
!
Œ5³ ÿ`(6LMÊîF
^[yv[ÙÚÚ6
#
¼mQRi¿Zíî
!
v[’jfE“i¹H
!
³ÎŠ45_1CóÏ
 »
#
LMã¤Ñã^ »Ÿ [)«
!
!½LMã
g˜ÊÓë

/{ùÓ5#Ëyv[Ù_ƒ„[‚
&ð™
!
±25z†/{
$2F/5I()0/)$&(&(G2%/<
V#/%(00!XÑÚêyv[ك„[+á6¡ù
Ó

t—¯×dÏØ[E“ÑÍЫ
!
ht—
&€Ãÿ~[Ǥ˜
!
¡Y™ìÿSór¿¢¹
Cyv
#
àiyv[’i
!
@Ìyv[\·[F^i
óÏ[€ã
#
€$3yj‰

£¦§B“yv[Ù
_CYZ¦§Ù[8‰Só#˃„
!
T)EGy
v[
!
Ö¶t—¯×dÏØSóÀ¦Ì
!
m]êŠ
.t—¯×dÏØE“[6ÃGìܵq¥)

! !
ÁÂ
!]! !
ßà
!-T>1(†Ú)N®6ß
$
úû
S‹ùÓ\“ƒ„²
%&;8@V#/%
#
A=
‡/&+H
å
#-2$)
A=
T>CA1K
 ›e/{Hå
#=4&2A(IV
%
ѵ†Ðy
#-81‘ài/{ß
$>I(%0#$I7/<
0G()G(0
˜%
!

%&
FGD¦¡áì
$ 1*I$
˜
%
%&;I$*(=$02(%XTV&$2)4IU(%LgM$
*CãÌn
üƒ„²
%&,I$KV/F(%&//W XRMM+C
â(†6Üß

!]#
çÛ
!
›û
!
A=
!A%0!
qãB
!.>81!TAA
‡+n
J&4W$
˜%
&1T1!A-=-T
ê
1*I$
6k
&
y
vÔ·çÛý
XI(%0#$I7/0G()G(0
˜%
%&
ïј™
#
ôzïј™ê
>I%(0G/
äC
&
;8@
†|
#;8@
 í¸
#
=/v[Œ+n
@-
˜%
&
ªÇçÛê6œ

!]$
ÇÌiÊÌ
!
t—+n¬üR[t—Ç
ÁãäsQ
!
ͬürýÇ?_ŸŽ¾¿ÕÔê
\¶øÉÌ\¶
;%/#2*)( M(K%/#2(
[=„—Î

/
K.¢8‰
!
b¬ürýÇ?_¦§ÊÌráµ
q
!
ÄÅÆf
1d+D$
­
%ôâ×dÏØ
J=
R
â‰Ç¯\ 
!
•nr­óÏ«KGÜráÏ
ØÏ«KGܲآ«

# !
…©i0Œ
#]!
×dÐâ8‰[Üß
!
’2è‰7«[…©
#˦§
R(

#]# !
t—ÇÁµ$¸[ÜÝ
!
$t—ÇÁ£
á
RMM *!
/¶®r
!

N
þ·[
LMh
§gÊ×µ
$
X
5
!
85
R #!
· 
!
·¸4O
$YM l%
>¤¬-
g>
!
Y5>¤>aQX ¬
MgL *-IC
SR
[á¸
!
±Œ
!
Õ

#]$ !
ÝÇi$S
!
$×dÐâ8‰
!
žìR6Ú
X
Ù
!
ªr|Ù]\¢×dÏØ
X #
¾
!
™š—
XM IC
-W*
SR
Û·µBÝ%t—µ$¸
$
ÐâÝÇÙ
%!
-R\MR-
{
QY
ï{
N
—
XMRR
÷
_
´
!!!!!! ! ! ! ! ! !!!! ! ! ! !
U/&gQY! ;004(!N
>V%&! XMRR
Ú|ÙÝ%Z‡Û·[ã¤ÓQEêÐâC2
&
Ú
$»Z8‰
RN
~
!
žìR6Ú
X
Ù
!
ªr|ٗ
XM
IC-W*
SR
[Û·µBÝ%t—µ$¸
$
»ZÝÇ
Ù
%!
Ú|ÙÝ%Z‡Û·[ã¤ÓQEêevC
2
&
_¿Ù8:
Q
5
!
ÐÝÇ
Q 5!
nb$»
!

|5ÝǾ
QM I)!
PÔÿk
!
P…?!
RMh

ä„
MgX IC

!
¬…ò$S
R IC
X$K[
8‰S¸«G4/~
_L I)!
Í(S
!Y MMM %-
I)
SR
¡á
RM I)!
8áI$_ØSó
!
/
-8
Vr
!
8‰[Só6ÙHjŽ4
! S\M l

!
ݓ

B
“
XCSóÈC#Ë
#]% !
z†(†/{6
$X†|Q^ч/
&+—
@/%*
‡[…©Ñ
;8@V#/%
#
A=
‡/&+Hå
þ#Ë
X(

{|†‡/&+/{’([¼
R&
{ý†
/{B“
-2$)
A=
T>CA1K
 ›e/{Hå
RXh1T1<
8>@-
/{

(†M}B““í=3fM}

ê4
g.E
!
8¢yvÈCkl
Q
5

M}¾[(†B“
;I$*(=$02(%
A=
XT8&$2)4I1/62F$%($U(%0/) LgM%

‰s#Ë(†f)6

0Œ¼.
!
ZÙ8‰Só[
XfŒè3·[jº(
!
YZ¦§Ù’[fŒè
|Ô[EG
!
Ø6yv-\·F^3?

¿¦§Ù
X<
T-
(†fŒ[f
R!
ÍْCŠƒ„
!
)K?[yv[-Ð
RL

!
yÿI/GWf[f
X
EG
yv]¿Ù/{fŒ[61[¼
X
¼
R!
‡/&+
$;-J%
’(
N /O
[U
7?/×
[
!
> ![#
6/O9’W
1R QM LM \ S
1X YM LM \ S
1Q XMM LM R S
1_ LMM LM R S
1L R MMM LM R S
1Y N MMM LM R S
1\ N MMM LM S S

S S S \X MMM
#]& !
EGyv[[n[·ñfŒ&Ô
!
Å$E
Gyv[-
#
uv
#
=„¾[n³Ä̓s[ŸgØ
KvI:Ë9’[Œ
$=>CT;#Ën[
·ñŒ
$V(V25(I$006)*(%V%)2)*!8=J%


#]
EGyv[-Ú6:Eƒ„
!
X\“
=>CT;<
AOJ<=1
Å[n[·ñfŒ
$8=J%
çÒ/N‰
s
=/E(%a
G
8($W-%$a/%
#˺2»>XW
A%BV0)
nßvºG`yvˆMº

X2»¾[fŒçÒ
!!
>:
evC2Ù
&7:
»ZÝÇÙ
&:
ÐâC2Ù
&T:
ÐâÝÇÙ

f
R!
¿¦§Ù
X(†fŒ
f
X!
EGyv[-I/GWf
¼
X!
EGyv[-]¿¦§Ù
X(†fŒr[61
Wf
evC

»ZÝ
ÇÙ
ÐâC

ÐâÝ
ÇÙ
R S q q q
X S q q q
(
Q S q
(
q S
_ S q q q
(
L S q q q
)
Y S q q q
\ S q q q
(
N S q q q
` q q q
)
S
RM q q q
)
S
RR q q q
)
S
RX S q q S
RQ S q q S
R_ S S q q
RL S q
(
q q
!!
?
,q
\è²yv[-
&<
Y\²yv[-
&q
(yv[-
\·jC3F
&q
)yv[-\·jC3·

?7;)%
()Û
$#2V,
*
FFF:I$2%K0G()G(:G/I%!
á
-X\MR-
{
QY
ï{
N
—
XMRR
÷
_
´
!!!!!! ! ! ! ! ! !!!! ! ! ! !
U/&gQY! ;004(!N
>V%&! XMRR
/j\’(¾#ËC½
RL
‚&EGyv[-
r
!
W
\!RQ!RL

!
ªÇ
RX
ŒÅÚ6/)

ÑÕÔ[Ú6r
X
fi
_
fyv[[ãsBeJ|
ð
&` iRR
fyv[[ãsBeJ|ð
!

Q
¼
Q!
EGyv[-[Ú6ÕÔ
Wf Ž7MP r7MP
R $V/&V/V%/2() >;=40I40G4&40(
‰pˆyv
>;
X $V/&V/V%/2() >;V%(G4%0/%=40I40G4&40(
‰pˆyv
>;
hÿ
Q !IK24%(, #$) >!G%B02$&02%4G24%(/6%(G/IP)$)2I$H/%I/40(4%)$<
%BV%/2() $3I4V% >2Rg\L >%(0/&42/)&$V/&V/V%/2()>;! 0/6/%I
3>
<
P =40I40G4&40(
!X
¢yv[³ÄÌ
,
|¢5kى‚›yv
&
|¢5‰
pˆyv
>;

3>
<
P
_ $V/&V/V%/2() >;V%(G4%0/%=40I40G4&40(
‰pˆyv
>;
hÿ
L $V/&V/V%/2() >;V%(G4%0/%=40I40G4&40(
‰pˆyv
>;
hÿ
Y #$) >! G%B02$&02%4G24%(/6I/40(2%$)02#B%(2)
‰Môíiyv
\ 4)W)/F)
Íï
N 4))$I(5 V%/2() V%/54G2=40I40G4&40(
ÍgM[|¢‰yv
` #$V2/*&/P) V%(G4%0/%=40I40G4&40(
‰0Äfyvhÿ
RM #$V2/*&/P) V%(G4%0/%=40I40G4&40(
‰0Äfyvhÿ
RR #$V2/*&/P) V%(G4%0/%=40I40G4&40(
‰0Äfyvhÿ
RX I$H/%4%)$%BV%/2() RR =40I40G4&40(
‰‚›yv
RR
RQ 4)W)/F)
Íï
R_ IK24%(,;*W$VV$G#$)&;*W$VV$&*#2G#$)
¹CÔyvsI1â
$X
¢
%
RL 4)W)/F)
Íï
$
80i‡,
£UDÙ&ƒ„€Tt—yè.w4s×d
ÏØßÚ[Ç{-E“
!
Ç(p܅mÅ<
R\gLRh!
i:èé4sdM8‰[’[ÇO[Ï
¤ÀF
Q<_(

‰pˆyv
>;$>V/>;%
‚&61•
.TC$
·3
(ˆyv
%!
5²ùgŸ˜M`u
$C>A%
ؘÛ
!
«
2
.TC
!ÿ

胄¼.
!.TC
i:pÜô‘ÏØ
i÷‚LM[fÄ
!
bipÜùíÏØ
$%/2$E%40%
C
mLM[(°
!
yè7Œ[¯ÏØE“
!.TC
*0
Ä
#
rÑ|HJèw¸Ñ-w¸[
T?>
Ñ
3?>
Ï
Ø
!
ü‹­qBÏØ
$)(FG$02&(50($0(E%40%#
Hœ]
?ÏØ
$#(%V(00IV&(KE%40%#
×dÏ؇
!
‚&µé
Ú6ê
.TC
r[‘¢pˆyv
L(
>V/>;
GÄÚ[
>V/>;
ªºÌ*pÜHœ]?ÏØæç[LMfÄ
!
³hpÜÏ؈Ò÷‚LM~Ø
L(

£¦§0Œw
½
>V/>;
G
>V/>;
hÿ]»ZÝÇÙ
#
ÐâC2ÙG
ÐâÝÇÙ[8‰SórŒ]w£6a¡˜
!
µ½t
—i:µ·
.TC
j‰yv[[¼m
!
pÜ×dÏ
؈Ò÷‚LM
!
³hmů×dE“

‰Môíiyv
$2%$)02#B%(2)%!
lPôíiû
0Ähvyv
!
C
A_
è·^ѝ
!
F0Ä·
!
5
ôíiØûSó0Äyv[|Ø6
!
i6¡îôí
iØûÊîRþ

ôíiØûis#Ì[ä^
!
6
aäž
!
µ·s¨ôօ‡
!
iìÿ¯O
#
¹CPµ
·‰H3y

²yv]»ZÝÇÙ
#
ÐâC2ÙG
ÐâÝÇÙ[8‰SórŒ]w£6a¡˜

@|
0Œµ½t—*m µ·ìÿ¯O
#
¹CP;_
¯×dÏØ

0Äfyv
$#$V2/*&/P)!
yP
.V%!
lP`Ô
yv
!
5Só#
ÔyvÙ6r[|¢Æyv
.V
5|¢Q—yv
!
yè0ÄSmyv
#
¯Ó
#
s#
SVãÚђi¹C\‡‘¢ài
Y(
d$)*
‡
\(
B“ÈI¥.ѹCrP‡…©Æ¦
!.V I3<
?>
*]dž(LMÑQB”LMG‹R
#â_€LMr¼m
!
]Çe‰¸4GdžZ+Ã
[¯ä^
#
¯ZãÌG{-SV[¿l‡…Vyè
k&E“

evC2Ù
#
»ZÝÇÙ8‰Só\è
²yv
!
]ÐâC2Ùr].w6a¡˜
!
h]Ðâ
ÝÇÙr¼mw£4Œ

²¦§0Œj.t—è
AYdž_€LM
#
0ldžLM[ài

‚›yv
RR$I$H/%4%)$%BV%/2() RR!=,8RR%
ê
&V/G$&)$
ê^ˆpÿyv
!
Eˆ[ëpyvª
!
;ÊèÓ4[r7MP
%
yvæ†Ú,g|
&<
V/G$&)
’iŸÚ<ìÿ~ô‘YZ[*ixZk
&[ãÌài
!
üu[ÄÚ
#
¹CXã
#
LM[nò
-Q\MR-
{
QY
ï{
N
—
XMRR
÷
_
´
!!!!!! ! ! ! ! ! !!!! ! ! ! !
U/&gQY! ;004(!N
>V%&! XMRR
Rþ‡
N(
=,8
yv]»ZÝÇÙÑÐâC2Ù
].w6a¡˜
!
h]evC2ÙÑÐâÝÇÙr
¼mw£4Œ

µ½t—G×dÏØ*X·²y
v[¼m·
!
5àit—¯×dÏØè‰
!
¼è_
#|NC
=,8RR
ãÌài[ƒ„

;*@
6`b
_
|nIÙÚ

ªrjC6`[·
ê
XgL
!
$XQ WT%
[nI
!
PsI
$C
I
%!
jC6`
[·ê
L
![nI
$LM iYM WT%!
PkI
$.
I
%
sIikIg’m ýAŠ
$<1<1<%
jW
8|
sI–ãsâO
$?
O
%R[X
)°~[ãsBÙÚe
J‘FÖêsI*F)
$UC%!
5
;*@
6`i¯È0
ÄØI[|ÙÚØ6

ªé
R[X
)°~[ãsB
ÙÚG£J”ejC#ÔÖêsI­Ô)
$C%
b•sI­Ô)~ãsBeJŽ]ÜÌEG
!
rs

P
Ñ&=â
R_
fyv[-²\[
X
¢yv
Õ5
C
I
P
â[
X
¢
!
E
;*@
6`[|Ø6

²Ú
6]evC2ÙÑ»ZÝÇÙr¼m3Œ
!
h]Ð
âC2ÙÑÐâÝÇÙ¼m6¬

¼.
;*@
!×d
ÏØ®¬¯È×ؼm·6a
!
t—C8‰¹
CHå€Ã¯\ȝ[z†XãE“
!
]»Zâ´
¬
!
C¹CHåkŽ-DE
&
]\Ø|s¬
!
*µ·
;*@
[¼m
!
µ·ìÿ¹CP

t—*m DEØ6Sóàiyv[¼m·
’\€Ã¯×dÏØE“
!
@Ìàiyv62E
•pˆyv
#
ôíiû0Ähvyv
#&V/G$&)#

fyv
#;*@
‡
!
m pÜÏ؈Ò÷‚LM
#
µ·
¹CP
#
¯O‡E“€Ã¯×dÏؘ

@Ìy
v[5t—]ìÿ~€Ã¯×dÏØE“[k&
’iF
!
b5ƒ„t—¯×dÏز&T)[S
óÀ¦Ì

$
’“”•
%
R(!
ÕH
:
rÇǤƒ„…©_
=(:
úû
,
±Ï½ãKÄæ
!
R``Q,XLR:
X(!@/%*>! OP(%I$(%! 7/*42# @! (2$&:3(G()25(E(&/VI()20)
2F/<5I()0/)$&(&(G2%/#/%(00F2# II/P<&a(5 V.*%$5()20,
b5(V.*%$5()204V 2/V.RX!&/)*(%0(V$%$2/) 502$)G(0$)5
0IV&6(5 V%/G(54%(09(:-&(G2%/V#/%(00!R```! XM$L%,\RX:
Q(!
ûW@
!
ŸŽ
:
t—¯8‰×dE“[Œé‰Hƒ„
9(:
rÇǤióô
!XMM`!XL$_%,LM:
_(!
ŸŽ
!
ûW@
:
t—ÿ®¯×dÏØèéØIƒ„
9(:
¬
ürýÇ?__2
!XMRM!Q_$X%,R\N:
L(!
·l
!
—Ñ`±@
!
cLì
:
·3(ˆyv]¯dMr[E
“
:
~´íð{?__2
!XMM`!QM$R%, X``:
Y(!C$)*&/0=3! T(&$)*#(93:7/&/*G$&$)5 G&)G$&0*)6G$)G(
/6#$V2/*&/P) V/&BI/%V#0I) #4I$)09(:&) #(I!R``Y! _X
$RM%,RLN`:
\(!d$)*J!J%(5%G#0b-!?$E$%H/>0#P$4*# >C! (2$&:(&2BV(<
0V(G6G$)5 )6&$I$I$2/%B<)54G(5 (KV%(00/) /6#$V2/*&/P) *()(
) C4)*9(:C$P ;)E(02!R``L! \Q$Q%,_QQ:
N(!T$%() 3:JCOb-3:A#(&V/G$&) V%/2() 6$I&B,02%4G24%($)5
64)G2/)9(:7/G#(I9! R``Y$QRN%, R:
=TGAAC/CD0-0CM1IACW/ R0GIKKAGI../GYAGBM<*#6$(& @&+*#6&
NF LGBDAB.0TKDATECB1BVF
c.>?@.4I)
R
! 1O?@9$)
X
! 1.;94)B)*
X
!
$RGC<(/0%/1 62(0*:%5@)(0#!#%/(4<#/*.*J*0#2#/*! T#/(/ XLMMR_! <#/(&
XGC<(/0%/1 8/#9*).#!:%5@)(0#!#%/(4<#/*.*J*0#2#/*! T#/(/ XLMQLL! <#/(%
!!6NKDG/TD(8N\ATD0RA, A#(G$)55$2($)2<)6&4()a$E%400(%4II$%W(%/6;%/#2*)( M(K%/#2( F$00($%G#(5 PBG/IV$%)*2#(0(<
%4IV%/2() 50G%(V$)GB/6(KV(%I()2$&*%/4V0:;2F&V%/E5(2#(P$006/%0(2)*6/%2# 2#($G2/) $)5 I(G#$)0I/6$)2<)6&4()a$E<
%40:>ADEBH,AF/<5I()0/)$&(&(G2%/V#/%(00$XV%/2() F$0G/IV$%(5:A#(0*)6G$)2&B(KV%(00(5 V%/2() 0V/20F$00(&(G2(5 6/%I$000V(G2%4I5()26G$2/) $)5 P/)6/%I$2G0$)$&B<
00::AKI1D, J62(() 5()26(5 V/)20/6V%/2() #$E(I/%(2BVG$&56(%()G(0P(2F(() (KV(%I()2$&*%/4V0:A#(02%4G24%(0F(%(5()2<
6(5 PB=>CT;P(2F(() 2#(0(%4IV%/2() /6(KV(%I()2$&*%/4V08%/2(/IGI(2#/50G$) P(40(5 2/0(&(G2$)5 5()26B2#(0(%4II$%W(%:;20(KV(G2(5
2/G&$%6B2#(I(G#$)0I/6$)2<)6&4()a$E%40/6;GM(K%/#2(
A^F XBGHK(!;%/#2*)( M(K%/#2(& V%/2(/IG0& )6&4()a$E%40& I$%W(%
HB0,!+_%#)QT\T..#+!!+)#)
$
–—$Q
!
´µµ
%
-_\MR-
{
QY
ï{
N
—
XMRR
÷
_
´
!!!!!! ! ! ! ! ! !!!! ! ! ! !
U/&gQY! ;004(!N
>V%&! XMRR