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作 者 ：陈玉静,李林,李宗信,黄小波,张丽,陈文强

期 刊 ：中国中药杂志 2011年 36卷 11期 页码：1519.

关键词：2型糖尿病；认知障碍；脑康Ⅱ号；细胞凋亡；

Keywords:type 2 diabetes, cognitive disorder, Naokang Erhao decoction, apoptosis,

摘 要 ：目的: 探讨脑康Ⅱ号对2型糖尿病大鼠认知功能, 海马CA1区Caspase-3, Bcl-2和Bax蛋白表达的影响及其治疗糖尿病认知障碍的可能作用机制。 方法 : 高糖高脂饮食结合腹腔注射链脲佐菌素诱发糖尿病大鼠模型, 并用3种不同剂量的脑康Ⅱ号治疗4周。采用Morris水迷宫法进行行为学检测,用免疫组化法检测海马CA1区凋亡相关蛋白Caspase-3, Bcl-2和Bax的表达水平。 结果 : 与正常对照组相比,糖尿病模型组Morris水迷宫平均逃避潜伏期和游泳距离均明显延长(P<0.01),空间探索实验中在原平台所在象限游泳时间明显缩短(P<0.01),表明学习记忆能力显著下降;模型组海马CA1区凋亡相关蛋白Caspase-3和Bax表达增多(P<0.01),Bcl-2表达减少(P<0.01)。经过脑康Ⅱ号各剂量组(分别含生药0.5,1.0,2.0 g·mL^-1)灌胃治疗4周,糖尿病大鼠学习记忆能力显著改善(P<0.01或P<0.05),海马CA1区Bcl-2表达显著上调(P<0.01或P<0.05),Caspase-3和Bax表达明显减少(P<0.01或P<0.05)。 结论 : 脑康Ⅱ号对2型糖尿病大鼠脑神经细胞的保护作用可能是通过上调Bcl-2蛋白、下调Caspase-3和Bax蛋白的表达,抑制细胞凋亡而实现,进而改善2型糖尿病大鼠的学习记忆能力。

Abstract:Objective : To investigate the effects of Naokang Erhao decoction on the cognitive ability and the expression of Caspase-3, Bax and Bcl-2 proteins in hippocampus of type 2 diabetic rats. Method : The diabetes mellitus (DM) rat model was produced by intraperitoneal injection of streptozotocin and fed with high fat and sucrose diet. The Naokang Erhao-treated rats were intragastrically given different doses of Naokang Erhao, whereas the control and DM model groups were given double distilled water for 4 consecutive weeks. Learning and memory abilities of rats were tested with the Morris water maze. The expression of Caspase-3, Bax and Bcl-2 proteins in hippocampal CA1 region was measured by immunohistochemistry. Result : Both escape latency and swimming distance of type 2 DM rats were significantly prolonged in comparison of those in normal control (P<0.01), and swimming time in the platform of previous quadrant was significantly shorter in model group (P<0.01). Furthermore, the expression of Bcl-2 protein was decreased, while Caspase-3 and Bax in the hippocampus were increased compared with the control group (P<0.01). Four weeks of treatment with Naokang Erhao decoction remarkably improved the learning and memory abilities of DM rats, increased the expression of Bcl-2 and decreased the expression of Caspase-3 and Bax in hippocampal CA1 region of model rats (P<0.01 or P<0.05). Conclusion : Naokang Erhao decoction may inhibit apoptosis by increasing the expression of Bcl-2 and reducing the expression of Caspase-3 and Bax in the hippocampus, and this may be one of the mechanisms by which Naokang Erhao decoction improves cognitive ability in DM rats.

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