作 者 :王然,唐生安,翟慧媛,段宏泉
期 刊 :中国中药杂志 2011年 36卷 8期 页码:1032.
关键词:山胡椒 ;生物碱 ;单萜 ;抗肿瘤转移活性 ;
Keywords:Lindera glauca , alkaloid , monoterpene , anti-tumor metastatic activity ,
摘 要 :目的: 研究山胡椒Lindera glauca 中的化学成分及其抗肿瘤转移活性。 方法 : 综合运用硅胶柱色谱、凝胶柱色谱、HPLC制备色谱等多种色谱法分离纯化山胡椒中的化学成分;采用NMR等波谱学方法鉴定化合物结构,进一步以Transwell 趋化实验方法测定化合物抗肿瘤转移作用。 结果 :从山胡椒乙醇提取物中分离得到10个化合物,分别为樟苍碱( 1 ),N- 甲基樟苍碱( 2 ),瑞枯灵( 3 ),紫堇碱( 4 ),N- 反式阿魏酸酪酰胺( 5 ),N- 顺式阿魏酸酪酰胺( 6 ),芒籽香碱( 7 ),降异紫堇定碱( 8 ), -(1S* ,3S* ,4S* ,8S* )-p -Menthane-3,8-diol( 9 ), -(1S* ,3R* ,4S* ,8S* )-p -Menthane-3,8-diol( 10 )。抗肿瘤转移实验结果表明化合物 1,2,4,5,7和9 均有明确的抗肿瘤转移作用,其中化合物 1,4和5 的抗肿瘤转移活性较强。 结论 :化合物 3 为首次从该植物中分离得到,化合物 9,10 为首次从该属植物中分离得到;化合物 1,4和5 的抗肿瘤转移活性较强。
Abstract:Objective : To study the anti-tumor metastatic constituents from Lindera glauca . Method : Constituent isolation and purification was carried by repeated column chromatography(silica gel,Toyopearl HW-40 and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The anti-tumor metastasis assay was applied to evaluate the isolated compounds of their activities. Result : Ten compounds( 1~10 ) were isolated and their structures were identified by comparison of their spectral data with literature values as follows: Laurotetanine( 1 ),N- methyllaurotetanine( 2 ),reticuline( 3 ),pallidine( 4 ),N- trans-feruloyltyramine( 5 ),N- cis-feruloyltyramine( 6 ),atheroline( 7 ),norisosocorydine( 8 ), -(1S* ,3S* ,4S* ,8S* )-p-menthane-3,8-diol( 9 ), -(1S* ,3R* ,4S* ,8S* )-p-Menthane-3,8-diol( 10 ). Compounds 1,2,4,5,7 and 9 showed positive anti-tumor metastatic activities,and compounds 1,4 ,and 5 showed significant anti-tumor metastatic activities. Conclusion : Compound 3 was isolated from this plant for the first time. Compounds 9 and 10 were isolated from Lindera genus for the first time. Compounds 1,4 ,and 5 showed significant anti-tumor metastatic activities.
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