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期 刊 ：中国中药杂志 2008年 33卷 03期 页码：307.

关键词：悬尾实验；强迫游泳实验；自主活动；抗抑郁；单胺氧化酶活性；减压特号；

Keywords:tail suspension test, forced swimming test, locomotor activity, antidepressant-like effect, monoamine oxidase, Jianyate Hao(JYTH),

摘 要 ：目的:探讨中药复方减压特号急性给药对ICR小鼠抑郁模型的拮抗作用及其可能的机制。方法:采用经典的行为绝望抑郁模型,即小鼠悬尾实验和强迫游泳实验,观察减压特号急性给药对应激小鼠绝望不动时间的影响;同时观察减压特号对小鼠自主活动的影响,荧光分光光度法测定减压特号对小鼠脑内单胺氧化酶活性的影响。结果:与空白组比较,减压特号(17.5,35,70 g.kg-11,ig)明显缩短悬尾和强迫游泳实验中小鼠的不动时间,其中35 g.kg-1剂量组作用类似于阳性药物丙咪嗪(10mg.kg-1,ip);减压特号(17.5,35,70 g.kg-1,ig)对小鼠的自发活动没有明显影响;减压特号(35 g.kg-1,ig)明显抑制小鼠脑内A,B两型单胺氧化酶活性。结论:减压特号急性给药在小鼠行为绝望模型中显示出明显的抗抑郁作用,其作用机制可能与抑制脑内单胺氧化酶活性,进而增加脑内胺类神经递质有关。

Abstract:Objective: Jianyate Hao(JYTH),a traditional Chinese medicine formula,which has been used effectively to treat depression in the past ten years.The purpose of this study was to explore the antidepressant effect of acute administration with JYTH and its possible mechanism.Method: the animals behavioral despair models of depression,the tail suspension and forced swimming tests,were used to explore the antidepressant effects of JYTH.In addition,the locomotor activity test was used to detect the change of locomotor activity.The monoamine oxidase activity in the mouse brain was also determined by using fluorospectrophotometry.Result: JYTH(17.5,35,70 g·kg-1,ig) could decrease the duration of immobility in both tail suspension and forced swimming tests,and the effect of JYTH(35 g·kg-1 ig) was resembling imipramine(10 mg·kg-1,ip) in relieving depression.And the effective doses(17.5,35,70 g·kg-1,ig) did not alter locomotion activity.Moreover,JYTH(35 g·kg-1,ig) was found to inhibit monoamine oxidase activity in the mouse brain.Conclusion: These foudings suggest that JYTH exerts antidepressant effect in animals behavioral despair tests and the underlying mechamism may involve the inhibition monoamine oxidase activity in the mouse brain.

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