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期 刊 ：中国中药杂志 2008年 33卷 04期 页码：392.

关键词：山茱萸；5-羟甲基糠醛；分离鉴定；生物活性；

Keywords:Cornus officinalis, 5-hydroxymethylfurfural, separation and appraisal, biological activity,

摘 要 ：目的:探讨山茱萸炮制后补肝益肾作用增强的物质基础及5-羟甲基糠醛(5-HMF)的生物活性。方法:结合药理实验与化学研究筛选出活性部位,并从炮制品二氯甲烷活性部位中分离鉴定出主要单体成分,并进行体内和体外药理试验,研究其生物活性。结果:单体化学成分为5-羟甲基糠醛,它对小鼠CC l4肝损伤模型和血管内皮细胞具有保护作用,抗氧化作用是其保护作用的机制之一。结论:5-羟甲基糠醛是山茱萸炮制品中的活性成分,是补肝益肾作用的物质基础之一。

Abstract:Objective: To develop the mechanism of improving protection function of prepared Cornus officinalis for liver and kidney and the biological activity of 5-hydroxymethylfurfural(5-HMF).Method: Pharmacological and chemical studies were used to choose active part.A compound from active part was separated and appraised.To investigate his biological functions,pharmacological experiment was actualized.Result: A component was separated and identified.His is 5-HMF.5-HMF can protect human vein epidermal cell against H2O2 and glucose and inprove acute liver injury in mice.Conclusion: 5-HMF is the active component in prepared Cornus officinalis and substance basis for protecting liver and kidney.

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