目的：研究藤茶双氢杨梅树皮素(APS)体内抗鸭乙肝病毒的作用。方法：选1日龄北京雏鸭，人工感染鸭乙型肝炎病毒(DHBV)。感染后第7天APS(70，150，300 mg·kg-1)口服给药，每天2次，共10 d。应用斑点杂交法观察用药前与用药后及停药后3 d血清中DHBV DNA的表达。结果：于药后5，10天，150, 300 mg·kg-1 APS对感染鸭血清DHBV DNA水平均有显著的抑制作用，且停药3 d未见明显的反跳现象。结论：APS有抑制乙肝病毒的作用。
Objective: We have evaluated the direct effect of ampelopsis (APS) on duck hepatitis B virus (DHBV) replication in ducklings in vivo. Method: One day old ducklings were infected with DHBV. After infection for 7 days, the animals were treated with APS at dosages of 70, 150, 300 mg·kg-1 of body weight via the oral route. The drug was given twice per day for 10 days continuously, and normal saline was used as control. The blood was drawn from the posterior tibial vein of all ducks before treatment (T0), after the medication for 5 (T5), 10 (T10) days and withdrawal of the drug for 3 days (P3). DHBV DNA in duck serum was detected by dot blot. Result: The duck serum DHBV DNA levels were reduced in the group of APS (150, 300 mg·kg-1) after treated for 5 and 10 days and the levels of DHBV DNA did not markedly relapse in both groups of APS after withdrawal of the drug for 3 days. We provide the first evidence that APS can efficiently inhibits DHBV replication in ducks in vivo. Conclusion: APS therefore warrants further investigation as a potential therapeutic agent for HBV infections.