免费文献传递   相关文献

Effects of triptolide on cell proliferation and regulationof Ras-MAPKs pathway in synoviocytes induced by tumor necrosis factor

雷公藤甲素对TNF-α诱导的滑膜细胞增殖的影响及对Ras-MAPKs信号转导通路的调控作用



全 文 :![<
!
5L-6Ç¡
H;I5
½¾Š789:;<
Šª«–¡
>BJ594EKJ
kAõ¾EVŠ”=>¬
‘’
!
“J”
!
•–—
!

!

ÏßÏÐà(Ó ÏÑùúû
!
ÍÎ
$$%$$#

de
#!
Qf
$
’“5L-6Ç
!HE
¡àf„:y
!H;I5


½¾ŠŽ,àŒ4lwxƒIMã789:
!>45FIG8
;
<Šª«–¡
>BJ594EKJ
kAõ¾EVŠ”=>¬
*
gh
#
š›³‚Š
HE!$c+($+c($+($&$ 20/R/G
g

†
>45FIG8
ÛM
$
œ¬
9H8
ÑzÇ~Â9:;<´
$
ŸEá
YNJ@NC2 \R/@
~š›³‚Š
HE
¡
H;I5
½¾Š
>45FIG8 >BJ594EKJ
kAõ¾E
V‹Œý­
!>BJ$A5E
)(
$A5T>K$A5V;K
hiÏJŠª«
*
ij
#9H8
déÍ6
$HE
Þl±‰˜÷_×
>45FIG8
Š;<
$
³_
×çµæ
$c+(f$c$f$)$c()f$&)c%%f+YNJ@NC2 \R/@
dél6
HE
Þ±‰˜÷_×
>BJ594EKJ
kAõ¾EV‹Œý­
Šhi
*
ik
#HE
ý_×
>45FIG8
;<
%
,+78lv
$
³µO×Þý†”=7
>BJ594EKJ
kAõ¾EVŠðC´GJŒ
*

lmn
#!
Ž,àŒ4l
+
ƒIMã789:
+
5L-6Ç
+ 94EKJ

op)(
#!+$$*5$5+

qrsQ
#!
789êÄ&aëìí
!%$#+$

tbuv
#!
!
 Y
$
/0W
$
´ô—¾@
$HNR# !$$ #&$#*+$
T50BOR# RO22B(((3#)-./0

uvwx
#!
SE3
$
óô/0—
$T50BOR#\B0\//$%+%3#)-./0
!!
Ž,àŒ4l
! C?NS0B@/OQ BC@?CO@OJ$>4
õ¬‘
JêÅz{±Í
$
l78»¼l;—
%
Œ4È
`Ðcƒ„™ª
*
àf„:y 4
!@S0/C2N.5
C/JOJPB.@/C$H;I5


õÖÍbÍáâ$ž±Š9:
yÅ¬
$
?†³?Öd‚*´G
>BJ
ý­
$
ÐÙ
2\A´îŽÎ´Gý­A„
!0O@/DN25B.@OnB@NQ
AC/@NO2 ZO2BJN$94EK
,-
* >BJ594EKJ
kAõ¾E
VðCõ
>4
J78lŠ»ä™ª
*
/03E
áñÎM
>4
$,5L-°±ŠMl§z{_×
´ƒµ5L-6Ç
!@COA@/ROQN$HE
,+-
¡ÖÙ>
Š
>45FIG8 >BJ594EKJ
kAõ¾EV´GÏJŠ
ª«
$
u’“³õs
>4
ŠµO×
*
!
Et
Y!
9:À
%
,˜†°±
!>45FIG8
–Õ¬>
a
!ŒE•$-,/01
l,
$
©‚
**c(f+H;I5

!>vW
LM
+9H8
!EC/0NDB
LM
+
CM
>BJ

!9NCZ
LM
+
M
A5T>K

%
M
A5V;K

%
M
A5E
)(

!

-#áXG˜„ûü
—÷M?C
:D_%
-#áXG˜„ûü—÷M
:D_
–;5äGbb±
!NRN.@C/DN2NCB@NQ .?N0ORS0O2NJ5
.N2.N$T«ºê78$ÏëÛ()JKLM
+
UCBQP/CQ
°±²
!
ú.³ay()JKLM
*
Y!
|
!
ڊNÿ¾[O
!8OD0B
LM
+
„û|
!UO/CBQ
LM
+W` 5`#<
äWø|
!
789€¬|
_
*
!
Ç
Y!
9:>
!>45FIG8
Cñ>yˆ
+ 00/R/
G
g
ù`y¥
%$$ /^0G
g
v–ǧ
($ /^0G
g
–NJ789:լ>a$
$
ˆ
)% i$f
+
$
®§à‚>[=>
*
š®9:J+Ž

$
ìk¬9:,
&
U@
(
U
*
Y! 9H8
Ñz;<Ç~Â
HE
¡
>45FIG8
;<Š
ª«
!
m¡B—¼w
>45FIG8
l
) h$
)
—
=
šù‘
y
*#
š>ì
*
>
# ?
9:.|*µÖlm´
»
#
¦­¡Ë»
%H;I5
½¾»
%H;I5

kHE
»
!$c+($+c($+($&$ 20/R/G
g
$
v»Ö
&
—Jc
š
$
ÒÍ>
&( ?
*
$
všß÷
$$

G$
ž
9H8
+$

G$
M
& ?$
tHÌÆ*
$
y„\z{~Â|
&*$
20
áÂÃmšßb‚
D*
Ö¦­¡Ë»9:;<´

$$f$
ÊmÓLcßà
HE
¡
>45FIG8
;<_
×ç
!f*
_×ç
j , g!D
ìk»
=D
ãä»
- h
$$f*
ìkžã
)
¨
*
Y&!YNJ@NC2 \R/@
Ç~Â
>BJ594EKJ
kAõ¾E
/(((/
u
)
’u
%
w
+$$
n
&
o
!!!!!! ! ! ! ! !!!!!! ! ! ! !
[/Rc)$ :JJSN!%
4ACOR$ +$$
V´GÏJ
!
9:á²
#
$Ã
H;I5
¡
>45FIG8
>BJ594EKJ
EV´GŠ6½¾>Æ
$
/0µæ
Ö
H;I5
½¾
$)$ 0O2$$#$+& ?
2

—>ÆØ
+
)_
H;I5
½¾m
>BJ594EKJ
EV´G5Š>
ÆؐЬQ°kØ
$
ñΚ›³‚
HE!$c+($
+c($+($&$ 20/R/G
g

¡
>BJ594EKJ
EVkAý
­´Gª«
*
YNJ@NC2
ý­]/
#
‘9:
$
ž
>:E4
ŽÐry
›÷ŽÐ
?$& $$$ C/0O2
g
$& i
¾[
0O2$
lm
9:jý­
$UCBQP/CQ
ÇÂý­³‚
$
v»m
)$

D
ý
­÷ã
$$f8W85E4_T
µ¾ý­
$
Wõ±Ê
E[WI
8
$
Ɋ¢Ü
+ ?$
žÂM
>BJ!s $$$
§M
A5E
)(
$
M
A5T>K$
M
A5V;K

!
µæ
s $$$$s$$$s
$$$& i
Má¾
$HU8H
8
$
žÂ-#áXG˜
„ûüŠÕM
$
ɊM
+ ?$HU8H
8
$
ž
T
M
%
ͪ
%
ê
*
f0Ԙ*¬
4RA?BTBJNI<
÷µƒ
0µ¶È Ðcµ¶
$
$Ã2^Ÿ=Šßb‚gµ^
!
Öv¬¨ý­]/¡Ë»Ÿ=Šßb‚gµ^‹¡

*
ìkžã
)
¨
*
Y)!
Bâá²
!
ìkB⬠$
Fq/
h6
$
œ¬[y
ǝµ¶$»ÆB⵶
*
&!

&Y!HE
¡
>45FIG8
;<Šª«
!
®ˆÍ%-mñ
Î
$
BC>m¡Ë»
>45FIG8
Nô‰?1‰
$
9:
^è+Ž
$
E
H;I5

!$

D/G
g

½¾*9:‹›H
U>Æm³‰è–^膡˻‹o
$
HςeÍ;
ž
*
†
H;I5
½¾»‹Ñ
$
žÂš›³‚
HE

+&
?
*9:B‰eÍ,…
$
Ÿ¶!š›â‚Šï‰
$
h
!Ü2¶<
$
9:øô‰ïƒšñ¡‰
$)# l&( ?
*
$HE+($&$ 20/R/G
g
»9:ïBï?
$
ŸÞ½9
:¢¤
!
f
*
Ð¬Q‰G
>45FIG8
9:´
$
ìkœ¬
9H8
ÇÂó;<´W
*
déÍ6
$
$c+( 20/R/G
g
HE
¡
>45FIG8
;<°eͪ«
$
Ù
³³‚ø
+c( 20/R/G
g

&$ 20/R/G
g
>
$
¡
>45FIG8
;<_×çø
c$f
lN@
&)c%%f$
†
H;I5
½¾»‹Ñ«JÍ΍ð
!#d$c$
?
#d
$c$+
›>
HE
š›³‚»ÅÆÑÒ
$
ð«æJÌ
ÍÎjß
!#d$c$*
Þ½
HE

>45FIG8
;<
Š>¬æJeͱ‰˜÷
!
h
*
&Y!H;I5
½¾š›>Æ¡
>45FIG8 >BJ594EKJ
kAõ¾EV´GÏJŠª«
!H;I5

!$

D/
G
g

ÿ>ÆÒÞùú
>BJ594EKJ
kAõ¾EVŠ
!!
f
!
š›³‚
HE

&( ?
*b-m
>45FIG8
䏕G
! h$$
h
!HE
¡
>45FIG8
;<Šª«
!
$
Fq/$.j)
µ»
,˜³‚
=20/R/G
g
D
_×ç
=f
¡Ë
g $c% q$c$+( g
H;I5

g
$c( q$c$$(

g
H;I5

kHE $c+( $c($# q$c$$ $c+(
+c(
$c%(* q$c$)+
+
c$
+(
$c# q$c$+(
)
)$c()
&$
$c&# q$c$$
)
&)c%%
!!
p
#
†¦­»ÑÒ

#d$c$+
†
H;I5
½¾»ÑÒ
+
#d
$c$$
)
#d$c$*
´G
$
I
0O2
iNØ
$
³$¡
T>K
ý­Š´G
5
$
õ¡Ë»Š
)c#(
I
$
Ù¡
E
)(
Š´G…
$
A
¡Ë»Š
c%
I
+)$ 0O2
*ý­ª‡GÏJ¨

$ ?
*{
V;K

ÙmkAý­hi‰ahÊ
¡ËÏJ
$# ?
*
V;K

ŠhihÊ¡ËÏJ
+
›>d
éÍ6
H;I5
¡
V;K

!E
&#

ý­Š´G5y
V;K
+
!E
&

Š´G
!
f
+ *
&Y&! HE
¡
H;I5
½¾
0O2 >45FIG8 >BJ5
94EKJ
kAõ¾EVkAý­hiÏJŠª«
!
š›³‚
HE!$c+($+c($+($&$ 20/R/G
g

>¬y
>45FIG8 +& ?$
ޚ›â‚_×
H;I5
½¾ùúŠ
>45FIG8 >BJ594EKJ
kAõ¾EVŠ´G
$
Iª¼
HE
³‚ŠGN³_×>¬ªÅž5
!
f
)*
/*((/
u
)
’u
%
w
+$$
n
&
o
!!!!!! ! ! ! ! !!!!!! ! ! ! !
[/Rc)$ :JJSN!%
4ACOR$ +$$
f+!H;I5

!$

D/G
g

½¾š›>Æ¡
>45FIG8 >BJ$
A5E
)(
$A5T>K
§
A5V;K
ý­hiÏJŠª«
f
)!
š›³‚5L-6Ç¡
>45FIG8 >BJ$A5E
)(
$
A5T>K$A5V;K
ý­hiÏJŠª«
)!
d††“†
5L-õst
>4
tÐ$žŠ$,
*
¼w|}ì
7®e
$
‡
>4
Šst$
$
5L-Þ,…?vU¨’A
ǧ
!
?

xÖMl,
$
I³æe͊NÐڅN<
,)-
$
Êq)”b!³™$},ýmU³×ˆ
*
ÄÙ
$
yJ
lm»wŠ/0b!5L-Š°±z{´ƒ
µ
...
Õ_=€G‚˜5L-6ÇýJЋz{
Œ4l?CÍ¥Šb—§bŒ
$
³O×Þý†³¡
>4
78§789:Š”4JŒ
,&-
*
/0°f”789
:kAõ¾UVr‚Ðc‹ŒµOג“
*
789:Š;<õ
>4
Š™ªÍ²*ïŬ
$
ÙEl9:y/
H;I5
§
:G5
õEÐ789:;
<Š°±¦’
*
El9:y°±EáA´
>BJ5
94EKJ
kAõ¾EVuì!³E9:;<>¬
*
í
/0ÑÒjŠ
)
Ÿ
94EK
kAõ¾EV
#
Ò
T>K$
V;K$E
)(
EV
$
‡9:;<§lvîå$bힱ>
¬
*
/0he
>4
Íӊ78»¼$
T>K$V;K$E
)(
2
94EKJ
Šª‡GÏJÍÎGN
,5#-
* T>K
°±
ýõ¦¾JkAŠ:çHb
$
”=9:Š;<
§µG
,%-
* T>K
Š™ð_×±
EW*($*
†9:Û
>*
$
Þ_×øK
%
3‘€?—¼yøA¾à
Š9:;<
*
¨m34
V;K
_×±
8E#$$+ )$ 0D
/ZD
g
/Q
g
ýhÚ5±Œ4l
!BQ1SnB2@BC@?CO@OJ$
44
èCŒ4à/–Œ4È`§`ƒ„
,(-
* E
)(
94EK
†lvîåϞ‹Œ
$
)_Š
E
)(
_×±ý
ÍÎ,+
H;I5
õay?CŠlvîå
,*-
*
/0b!
H;I5

!$

D/G
g

ÞEÐ78ƒ
IM9:Š;<
$
ŸÞ½¾
94EKJ)
ŸkAõ¾E
V´G
$
&l6
H;I5

>45FIG8
Š;<†´G
†
94EKJ)
ŸkAõ¾EVJŒ
*
³$
H;I5
†9
:>¬
0O2
>
$
&
)
ŸEV´GÏJiNØ
$
ëe
H;I5
Þlªÿ´G
>BJ594EK
EV
$
ÐÙùúmÿ
x Šõקýþ
$
3
>45FIG8
­Í´G§;<
*
94EKJ
Š
)
ŸkAõ¾EVÞøš›ŠøA±A
´
$
‰ƒš›Š´GEV
$
õך›Š9:y
$
›>
0Þl‡š›ÏJ÷!‡^2”=
$
ì!*›>¬
*
Þ½
$
÷Ô
)
ŸT‹æé¡
$
!‡š›ÏJŠ‹æ

$
”Ù¾à
H;I5
‡
>4
ͲU4
$
è³õlv
îå§9:;*
çy 5
U!2S.RNBCPB.@/C5ZBAABU$ ;I5
5
U
ޔ=
H;I5

$:G5#
29:yŠõקhi
$
I
94EKJ
kAõ¾EV†
;I5
5
U
0!‡‹æ”4
*
J`•he
T>K
™ð_×±
EW*($*
ý_×
>4
ÍÓ789:
;I5
5
U
´4a
E
#
çõ×
,$-
$
ÐÙ

;I5
5
U
´G–j‘l9:y/
H;I5

$
:G5
2Šµ6
+
789:$
E
)(
0>;4
†
;I5
5
U
Š
hiÅÆ!‡eÍB‹Œ
,-
*
TF/0Í6
HE
ý
eÍ_×El9:y
:G5$H;I5

$:G5#
‡ÖÙ
>Š78ƒIM9:$Šhi
,&-
$
0ým”
<:4
?
CŒ4789:§È`9:$
;I5
5
U
Šhi
,+-
$

¨ìk/00b!
HE
ý±‰˜÷Š_×789:
;<–
T>K$V;K$E
)(
94EKJ
‡
>4
789:$Š
´G
*

$
·Â
HE
ÞýõEálmUV_×7
/$*(/
u
)
’u
%
w
+$$
n
&
o
!!!!!! ! ! ! ! !!!!!! ! ! ! !
[/Rc)$ :JJSN!%
4ACOR$ +$$
89:Š;<§lvîåŠ
#
%;ù_×789:
1—El9:y
+
&;ù_×
>BJ594EKJ
mÿ
)
—°±kAõ¾x Š´G
+

;I5
5
U$
”ÙÆ
ù_×El9:yŠ1—§
94EKJ
kAEVŠ
´G
*
/0Í6
HE
¡ÖÙ>Š789:
94EK
kAõ¾EVŠ´GæJeÍ_×>¬
$
l6_×
789:
>BJ594EKJ
kAõ¾EVŠ´GÞýõ
HE
_×789:;<
%
,+78lv”Ùst
>4
ŠµO×Ŭ
*

—˜
#!YNJ@NC2 \R/@
ìkp@_è-6
%
78
*§-.as-.,²É¤~~wx/0ɊV­
*

yz{|
#
, -!_wC@XU$ FB6NC8$ HSNC.Z U$ N@BRcHS0/SC2N.C/JOJPB.@/CB.@O5
nB@NJ@?N0O@/DN25B.@OnB@NQ AC/@NO2 ZO2BJNJA)(BRA?BB2Q T>KO2
@?NJ62/nOBR0N0\CB2NO2 nOn/,V-c4C@?CO@OJ>NJH?NC$ +$$$ %
!# &$c
, + - !aOS W$ KB/E;c:00S2/JSAACNJJOnNB2Q B2@O5O2PRB00B@/C6
0N.?B2OJ0J/P@COA@/ROQN$ @?NACO2.OABRB.@OnNQO@NCAN2/OQ PC/0
@?NWCSDJ>W$ +$$)$&!# c
, ) -!
î(ö
$
`Ïñ
c
¡5L-ŠÁ‘K
,V-c
$%$S-d‚,
àÍ23
$**#$!)##*c
, & -!GO2 ;$ 8B@/H$ :@/4cHCOA@/ROQN$ B2/nNRQO@NCAN2/OQ @CONA/7OQN
PC/0I()A0-(&;),8T)%65(?)F//ZcPc$ JSAACNJJNJ@?NAC/QS.@O/2
B2Q DN2NN7ACNJJO/2 /PAC/50B@CO70N@BR/AC/@NO2BJNJ B2Q ) B2Q
BSD0N2@J@?/JN/P@OJJSNO2?O\O@/CJ/P0N@BR/AC/@NO2BJNJ B2Q + O2
?S0B2 J62/nOBRPO\C/\RBJ@J,V-c4C@?CO@OJ>?NS0$ +$$$ &&!*#
+*)c
, -!V/?2J/2 _G$ GBABQB@>c9O@/DN25B.@OnB@NQ AC/@NO2 ZO2BJNAB@?5
,B6J0NQOB@NQ \6T>K$ V;K$ B2Q A)( AC/@NO2 ZO2BJNJ,V-c8.O5
N2.N$ +$$+$ +*(!#$$# *c
, # -!8,NN2N68 T$ IOCNJ@NO2 _8c8OD2BR@CB2JQS.@O/2 O2 C?NS0B@/OQ
BC@?CO@OJ,V-c?NS0B@/R$ +$$&$ #!)# +)c
, % -!9BCJ?BR@CB2JON2@nNCJSJJSJ@BO2NQ N7@CB.NRSRBCJOD2BR5CNDSRB@NQ ZO2BJNB.5
@OnB@O/2,V-c, ( -!FB2 ]$ U/6RNWG$ bSOCNQ P/C0N@BR/AC/@NO2BJNN7ACNJJO/2 B2Q 1/O2@QNJ@CS.@O/2 O2 O25
PRB00B@/C6BC@?CO@OJ,V-cV, * -!],NCO2BV$ FB6NC8$ >NQRO.? K$ N@BRc4.@OnB@O/2 /PA)( 94EK
OJBZN6J@NA O2 @S0/C2N.C/JOJPB.@/C50NQOB@NQ O2PRB00B@/C6\/2N
QNJ@CS.@O/2 ,V-c4C@?CO@OJ>?NS0$ +$$#$ &!+# &#)c
,$-!_/0NXEI$ EORO2DNC9F$ 4@SC9$ N@BRc>NJ/RS@O/2 /PO2PRB05
0B@O/2# EC/J@BDRB2QO2 T
+
QOJJ/.OB@NJ2S.RNBC@CBPO.ZO2D/PO2QO5
nOQSBR;I5ZBAABUJS\S2O@J!A#$ A$ O2 J@O0SRB@NQ C?NS0B@/OQ
J62/nOBRPO\C/\RBJ@J,V-cV:00S2/R$ +$$$ %!$# #*+&c
,-!
ðT
$
 Q
c
îŽÎ´Gý­A„–çy 5
U
‡5±Œ
4lèC78$Šhi
,V-c
$_,àÍ23
$+$$)$%
!(# $#c
,+-!GO2 ;$ GOS <$ LOB/<$ N@BRcHCOA@/ROQN$ BQO@NCAN2/OQ @CONA5
/7OQN$ JSAACNJJNJO2PRB00B@O/2 B2Q .BC@ORBDNQNJ@CS.@O/2 O2 ./RB5
DN25O2QS.NQ BC@?CO@OJ0O.N,V-cUO/.?N0E?BC0B./R$ +$$%$ %)
!# )#c
*K?TBI@KBE.JB@/.F?@AT?/JE@/.K?E-B.@A-AFE?SG/-B.@A
@K8-I+<4MXIJ-BCR-D .AIDA@P.@TDB?I.AFGT?FLD BG,@EA?TE@I.IK-TB@E
Y4;_VOB2X?S$ G:4;VO2CB/$ KM;_LOB2D6O2D$ G:;;B
!
!74).+ O./0)0,0-5674).-/->+0-()+ >-?)+%$ 74).+ D+?-8&5674).-/->-?)+%)-.-/$ 9-):).; $$%$$$ 74).+
,4LIBE-TB-!6LW?TB.P?# H/O2nNJ@ODB@N@?NNPN.@J/P@COA@/ROQN/2 AC/ROPNCB@O/2 B2Q CNDSRB@O/2 /PCBJ594EKJAB@?,B6O2 ?S0B2 PO5
\C/\RBJ@5ROZNJ62/nO/.6@NJ/PC?NS0B@/OQ BC@?CO@OJ!>45FIG8 @CNB@NQ ,O@? @S0/C2N.C/JOJPB.@/C!H;I5

c45FIG8 ,NCN
.SR@SCNQ ,O@? H;I5

O2 @?NACNJN2.N/CB\JN2.N/PnBCOB\RNQ/JNJ/P@COA@/ROQN!$c+($ +c($ +($ &$ 20/R/G
g
). =)0(5cB@O/2 ,BJNnBRSB@NQ \69H8 BJJB6cH?NA?/JA?/C6RB@O/2 J@B@SJ/P>BJ594EKJ5BJJ/.OB@NQ AC/@NO2J!>BJ$A5E
)(
$A5T>KB2Q A5V;K ,NCN
QN@N.@NQ \6YNJ@NC2 \R/@B2BR6JOJc8?IG/B# HCOA@/ROQN./SRQ /\nO/SJR6QN.CNBJN@?N>45FIG8 nOB\ORO@6O2 BQ/JN5QNAN2QN2@0B22NCB2Q
@?NO2?O\O@O/2 CB@O/,BJ$c+(f$ c$f$ )$c()f B2Q &)c%%f CNJAN.@OnNR6c:2 BQQO@O/2$ @COA@/ROQN./SRQ BRJ/JSAACNJJNQ @?NN7ACNJ5
JO/2 /P>BJ$ A5E
)(
$ A5T>KB2Q A5V;Kc0@AT/GI.@A# HCOA@/ROQN?BJB2 2/@B\RNO2?O\O@O2DNPN.@/2 AC/ROPNCB@O/2 /P>45FIG8 B2Q @?N
0/RN.SRN0N.?B2OJ0OJQSNO2 ABC@@/@?NQOCN.@JSAACNJJO/2 /PB\2/C0BRB.@OnB@O/2 /P>BJ594EKJAB@?,B6c
,X?D R@EFI-!C?NS0B@/OQ BC@?CO@OJ+ ?S0B2 PO\C/\RBJ@5ROZNJ62/nO/.6@NJ/PC?NS0B@/OQ BC@?CO@OJ+ @COA@/ROQN+ 94EKJ
F@.# ([)Z\OTWT,,(((%#

}~-0
!
²³³
#
/*(/
u
)
’u
%
w
+$$
n
&
o
!!!!!! ! ! ! ! !!!!!! ! ! ! !
[/Rc)$ :JJSN!%
4ACOR$ +$$