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作 者 ：韩淑英,王志路,储金秀,朱丽莎,熊国顺,杨云霞

期 刊 ：中国中药杂志 2009年 34卷 23期 页码：3114.

关键词：荞麦花叶黄酮；2型糖尿病；胰岛素抵抗；PTP1B；

Keywords:flavones of buckwheat flower and leaf, type 2 diabetes mellitus, insulin resistance, PTP1B,

摘 要 ：目的：探讨荞麦花叶黄酮（FBFL）对2型糖尿病大鼠降血糖、改善胰岛素抵抗的作用及其机制。方法： 雄性健康Wistar大鼠70只，随机抽取10只作为正常对照组，其余60只大鼠每天灌胃脂肪乳，第14天开始腹腔注射小剂量四氧嘧啶，隔日1次，共3次，同时继续灌胃脂肪乳。末次腹腔注射四氧嘧啶72 h后，用血糖仪测定空腹血糖和用K_IPT值判定胰岛素抵抗性。K_IPT＜正常组的60％作为2型糖尿病胰岛素抵抗大鼠。将成模大鼠随机分为模型组，阳性药物组，FBFL低、中、高剂量组，连续4周。末次给药后禁食，用血糖仪测空腹血糖(FBG)，用正糖钳技术判定胰岛素抵抗性，用生化分析仪测定游离脂肪酸(FFA)，放免法测定血浆胰岛素(INS)，免疫组织化学法检测肝组织蛋白酪氨酸磷酸酶1B（PTP1B）表达。结果：与正常组比较，模型组大鼠FBG，INS，FFA含量明显升高（P<0.05，P<0.01）；大鼠葡萄糖输注速率(GIR)明显下降；肝组织PTP1B 表达增加。各剂量FBFL能不同程度的改善上述指标的变化，明显改善胰岛素抵抗性，增加胰岛素敏感性，使肝组织PTP1B表达下调，并呈一定剂量依赖性。结论：FBFL对四氧嘧啶加脂肪乳所致2型糖尿病大鼠胰岛素抵抗具有明显的改善作用，并呈一定剂量依赖性。

Abstract:Objective： To explore the effect and mechanism of flavones of buckwheat flower and leaf (FBFL) on lowering blood glucose and improving insulin resistance in type 2 diabetic rats. Method： Seventy healthy male Wistar rats were used in this trial. Ten of them were selected randomly as normal group； the others were given fat milk by intragastric administration daily，from the 14th day on ，low dose tetraoxypyrimidine was added by intraperitoneal injection every other day for three times. Rats with fasting (72 hours after the last injection) blood sugar ≥16.7 mmol·L^-1 and KIPT < 60％ of normal group were selected as type 2 diabetic model with insulin resistance，which were randomly divided into 5 groups： model group. LGLT group； low，moderate and high dosage FBFL groups (L-FBFL； M-FBFL； H-FBFL). Every rat was treated accordingly for 4 weeks； then FBG，FFA，INS were detected and ISI was calculated to evaluate the degree of insulin resistance. Liver PTP1B expression was determined by immunohistochemistry method. staining were observed by light microscopy. Result： FBFL could dose-dependently inhibit the rising of FBG，FFA，INS，improve the state of insulin resistance and reduce the expression level of liver PTP1B. Conclusion： FBFL could effectively improve insulin resistance in type 2 diabetic rats induced by tetraoxypyrimidine and fat milk and showed dose-dependence relationship.

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